Myotis
myotis (Borkhausen, 1797) and Myotis
blythii (Tomes, 1857) are genetically close species of
bats with very similar morphology and karyology. In this study, specific
descriptive markers of 3 species (Myotis myotis,
Myotis blythii, and M.
cappacinii) belonging to the genus Myotis were determined by
using ISSR technique for the first time. In total, 91 bands including 86
polymorphic bands were obtained from the 7 best-optimized primers out of the analyzed 16 primers. UPMGA cluster
analysis showed 4 separate clusters. According to these results, the M. myotis and M. blythii were divided into two close groups, whereas
a single individual belonging to M.
myotis colony formed the third
group. M. cappacinii was in a more distinct and remote group with
respect to sibling species. Genetic diversity for all populations was
calculated as 94.5%. ISSR-PCR method was shown to be a reliable and useful
technique for detecting genotypic similarities/differences of Myotis species.
Primary Language | English |
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Journal Section | Articles |
Authors | |
Publication Date | October 26, 2019 |
Published in Issue | Year 2019 Volume: 14 Issue: 4 |