Genetic Divergence Patterns of Two Sibling Species (Myotis Myotis, Myotis blythii) and Myotis Cappacinii (Mammalia: chiroptera) in Central Anatolia Region

Year 2019, Volume: 14 Issue: 4, 49 - 57, 26.10.2019

Tuba Yağcı Nursel Aşan Baydemir

Abstract

Myotis
myotis (Borkhausen, 1797) and Myotis
blythii (Tomes, 1857) are genetically close species of
bats with very similar morphology and karyology. In this study, specific
descriptive markers of 3 species (Myotis myotis,
Myotis blythii, and M.
cappacinii) belonging to the genus Myotis were determined by
using ISSR technique for the first time. In total, 91 bands including 86
polymorphic bands were obtained from the 7 best-optimized primers out of the analyzed 16 primers. UPMGA cluster
analysis showed 4 separate clusters. According to these results, the M. myotis and M. blythii were divided into two close groups, whereas
a single individual belonging to M.
myotis colony formed the third
group. M. cappacinii was in a more distinct and remote group with
respect to sibling species. Genetic diversity for all populations was
calculated as 94.5%. ISSR-PCR method was shown to be a reliable and useful
technique for detecting genotypic similarities/differences of Myotis species.

Keywords

Genetic Differentiation, ISSR, ISSR, Chiroptera, Sibling, Myotis

References

• [1] Moreira, P.R.L. and Morielle-Versute, E., (2006). Genetic Variability in species of Bats Revealed by RAPD- Analysis. Genetics and Molecular Research, 5(4):804-815.
• [2] Reddy, M.P., Sarla, N., Neeraja, C.N., and Siddiq, E.A., (2002). Inter Simple Sequence Repeat (ISSR) Polymorphism and its Application in Plant Breeding. Euphytica, 128:9–17.
• [3] Wang, B., Zhang, Y., Chen, C.B., Li, X.L., Chen, R.Y., and Chen, L., (2007). Analysis on Genetic Diversity of Different Salvia Miltiorrhiza Geographical Populations in China. Chin J Chin Mater Medica, 32:1988–1991.
• [4] Bugarski-Stanojevi´c, V., Blagojevi´c, J., Stamenkovi´c, G., Adna –devi´c, T., Gia-Gia-Athanasopoulou, E.B., and Vujoˇsevi´c, M., (2011). Comparative Study of the Phylogenetic Structure in six Apodemus Species (Mammalia Roden-tia) Inferred from ISSR-PCR Data. Systematics and Biodiversity, 9(1):95-106.
• [5] Bugarski-Stanojević, V.J., Blagojević, T., Adnađević, V., and Jovanović Vujošević, M., (2013). Identification of the Sibling Species Apode-mus sylvaticus and Apodemus flavicollis (Rodentia, Muridae) Comparison of molecular methods. Zool. Anz., 252:579–587.
• [6] Yağcı, T., Gurbanov, R.R., and Şen, E., (2018). Using ISSR Markers in Determination of Genetic Relationship Between 2n=54 and 2n=60 Cytotypes of Nannospalax xanthodon (Nordmann, 1840) (Mammalia, Rodentia) from Central Anatolia. Russian Journal of Theriology, 17(2):100-107.
• [7] Bickford, D., Lohman, D.J., Sodhi, N.S., Ng, P.K., and Meier, R., (2007. Cryptic Species as a Window on Diversity and Conservation. Trends in Ecology & Evolution, 22:148–155.
• [8] Nygren, A., (2014). Cryptic Polychaete Diversity: A Review. Zoologica Scripta, 43(2):172–183.
• [9] Burgin, C.J., Colella, J.P., Kahn, P.L., and Upham, N.S., (2018). How many species of Mammals Are There? Journal of Mammalogy, 99:1-11.
• [10] Spitzenberger, F., (1996). Distribution and Subspecif c Variation of Myotis blythii and Myotis myotis in Turkey (Mammalia: Vespertilionidae). Ann. Naturhist. Mus., 98:9-23.
• [11] Simmons, N.B., (2005). Order Chiroptera. In: Mammal Species of the World. A Taxonomic and Geographic Reference, vol.1, edited by Wilson DE & Reeder DME. pp.312–529. Washington, DC: Johns Hopkins University Press.
• [12] Benda, P., Andreas, M., Kock, D., Lučan, R.K., Munclinger, P., Novă, P., Obuch, J., Ochman, K., Reiter, A., Uhrin, M., and Weinfurtová, D., (2006). Bats (Mammalia: Chiroptera) of the Eastern Mediterranean. Part 4. Bat Fauna of Syria: Distribution, Systematics, Ecology. Acta Soc. Zool. Bohem., 70:1-329.
• [13] Knowlton, N., (1993). Sibling Species in the Sea. Annual Review of Ecology and Systematics, 24(1993):189–216.
• [14] Ruedi, M. and Mayer, F., (2001). Molecular Systematics of Bats of the Genus Myotis (Vespertilionidae) Suggests Deterministic Ecomorphological Convergences. Molecular Phylogenetics and Evolution, 21:436–448.
• [15] Berthier, P., Excoffier, L., and Ruedi, M., (2006). Recurrent Replacement of mtDNA and Cryptic Hybridization Between two Sibling bat Species Myotis Myotis and Myotis blythii. Proceedings of the Royal. Society of London B: Biological Sciences, 273:3101–3123.
• [16] Bogdanowicz, W., Van Den Bussche, R.A., Gajewska, M., Postawa, T., and Harutyunyan, M., (2009. Ancient and Contemporary DNA Sheds Light on the History of Mouse-eared Bats in Europe and the Caucasus. Acta Chiropterologica, 11:289–305.
• [17] Bachanek, J. and Postawa, T., (2010). Morphological Evidence for Hybridization in the Sister Species Myotis Myotis and Myotis oxygnathus (Chiroptera: Vespertilionidae) in the Carpathian Basin. Acta Chiropterologica, 12:439–448.
• [18] Çoraman, E., Furman, A., Karataş, A., and Bilgin, R., (2013). Phylogeographic Analysis of Anatolian Bats Highlights the Importance of the Region for Preserving the Chiropteran Mitochondrial Genetic Diversity in the Western Palearctic. Conservation Genetics, 14:1205-1216.
• [19] Uhrin, M., Benda, P, Obuch, J., and Danko, S., (2008). Lesser Mouse-eared bat (Myotis blythii) in Slovakia: Distributional Status with Notes on its Biology and Ecology (Chiroptera: Vespertilionidae). Lynx, 39(1):153-190.
• [20] Piksa, K. and Woloszyn, B.W., (2001). Postglacial Bat Remains from the Polish Tatra caves. Lynx, 32:301–311.
• [21] Arlettaz, R., Christe, P., Lugon, A., Perrin, N., and Vogel, P., (2001). Food Availability Dictates the Timing of Parturition in Insectivorous Mouse-eared bats. Oikos, 95:105–111.
• [22] Asan, N., Albayrak, I., and Yorulmaz, T., (2010). Noteworthy New Records and Conservation of Myotis myotis (Borkhausen, 1797) and Myotis blythii (Tomes, 1857) (Mammalia: Vespertilionidae) in Turkey. Lynx, 41:145-150.
• [23] Southern, H.N., (1964). The Handbook of British Mammals. Blackwell Scientific Publications. Oxford, 342pp.
• [24] Mahoney, R., (1973). Laboratory Techniques in Zoology. 2nd edition. Butterworths, London, 120pp.
• [25] Arlettaz, R., Ruedi, M., Ibanez, C., Palmeirim, J., and Hausser, J., (1997). A New Perspective on the Zoogeography of the Sibling Mouse-eared Bat Species Myotis myotis and Myotis blythii: Morphological, Genetical and Ecological Evidence. J. Zool. Lond., 242:45-62.
• [26] Dietz, C. and Kiefer, A., (2014). Die Fledermӓuse Europas: Germany, Kosmos Naturführer, p.394.
• [27] Nei, M., (1978). Estimation of Average Heterozygosity and Genetic Distance from a Small number of Individuals. Genetics, 89:583-590.
• [28] Yeh, F.C., Yang, R.C., and Boyle, T., (1999). Popgene Version 1.31 Microsoft Windows-based Software for Population Genetics Analysis. Alberta Kanada. University of Alberta and Center for International Forestry Research, 28p.
• [29] Balasaravanan, T., Chezhian, P., Kamalakannan, R., Ghosh M., Yasodha, R., Varghese, M., and Gurumurthi, K., (2005). Determination of Inter- and Intra-species Genetic Relationships among Six Eucalyptus Species based on Inter-simple Sequence Repeats (ISSR). Tree Physiology, 25:1295–1302.
• [30] Karataş, A., (2019). The Bats (Mammalia: Chiroptera) of the Central and Eastern Mediterranean Region. Acta Biologica Turcica, 32(1):42-52.
• [31] Hoffmann, F.G., Owen, J.G., and Baker, R.J., (2003). mtDNA Perspective of Chromosomal Diversification and Hybridization in Peters’ Tent-making bat (Uroderma bilobatum: Phyllostomidae). Mol. Ecol., 12:2981–2993.
• [32] Afonso, E., Goydadin, A.C., Giraudoux, P., and Farny, G., (2017). Investigating Hybridization between the Two Sibling Bat Species Myotis myotis and M. blythii from Guano in a Natural Mixed Maternity Colony. PLoS ONE, 12(2):1-16.