Chia Tohumu ve Probiyotik/ Enzim İlavesinin Ayrı ve Kombine Olarak Tuj Koyunları Rasyonlarında Kullanımının Performans, Rumen ve Bazı Kan Parametreleri Üzerine Etkisi

Öz

Chia (Salvia hispanica L.), Labiate ailesinin bir üyesidir ve tohumları, çiftlik hayvanlarının performansını ve üretkenliğini artırabilecek fenolik bileşikler ve çoklu doymamış yağ asitleri (PUFA'lar) bakımından zengin, antioksidan özelliğindedir. Probiyotikler ise hayvan sağlığı ve büyüme performansı üzerinde farmasötik ve/veya metabolik etkilere sahip olabilecek yem katkı maddeleridir. Etki tarzlarındaki farklılıklar nedeniyle, bunların kombinasyonları ruminant hayvanlarda performans, kan ve rumen parametreleri üzerinde ilave etkilere sahip olabilir. Bu amaçla, çalışmada Tuj koyunları (n=20; 1,5 yaşında; ortalama 40,5 kg) her birinde 5 hayvan bulunacak şekilde 1 kontrol ve 3 deneme grubuna ayrılmıştır. Çalışma rasyonlarına ayrı ve kombine olarak, sırası ile 0, %2,5 chia tohumu (CT), 1g/kg probiyotik enzim karışımı (P+E) ve %2,5 CT + 1g/kg P+E ilavesi yapılmıştır. Koyunlar 7 gün adaptasyon ve 14 gün deneme süresi olacak şekilde toplam 21 gün beslenmiştir. Sonuçlar göstermiştir ki, Tuj koyunlarının rasyonlarına CT ve/veya P+E ilavesinin besi performansı üzerine etkisinin önemsiz bulunmuştur (P>0,05). Rumen toplam uçucu yağ (tVFA) asitleri konsantrasyonu kontrol grubuna göre artarken (P<0,05), rumen pH’sı etkilenmemiştir (P>0,05). Serum Esterleşmemiş yağ asitleri, Betahidroksibütrikasit, Nitrik oksit ve kalsiyum, değerleri katkı maddesi ilavesi yapılan deneme gruplarında kontrol grubuna göre farklılık gösterirken (P<0,05), serum Malondialdehit, fosfor, total protein ve glikoz konsantrasyonları üzerine herhangi bir etkisi olmamıştır (P>0,05).

Anahtar Kelimeler

• Chia
• Tuj koyunu
• Probiyotik
• Performans
• Rumen Parametreleri
• Kan Parametreleri

Effect of Single or Combined Dietary Supplementation of Chia Seed and Probiotic/Enzyme on Growth Performance, Ruminal and Some Blood Metabolites of Tuj Sheep

Abstract

Chia (Salvia hispanica L.) seeds have antioxidant properties that can improve livestock performance and productivity. Probiotics are feed additives that may have pharmaceutical and/or metabolic effects on animal health and growth performance. Due to the differences in their mode of action, it is thought that the combined use of chia seeds, probiotics and enzyme mix will cause additional effects on performance, blood and rumen parameters in ruminant animals. For this purpose, Tuj sheep (n=20; 1.5 years old; average 40.5 kg) were divided into 1 control and 3 experimental groups, each with 5 animals. Trail rations were separately and combined with 0, 2.5% chia seeds (CT), 1g/kg probiotic enzyme mixture (P+E) and 2.5% CT + 1g/kg P+E were added. Sheep were fed for a total of 21 days, with 7 days of adaptation and 14 days of trial period. The results indicated that the effect of adding CT and/or P+E to the diets of Tuj sheep on fattening performance was found to be insignificant (P>0.05). While rumen total essential oil (tVFA) acid concentration increased in the control experimental group (P<0.05), rumen pH was not affected (P>0.05). While the concentrations of serum, non-esterified fatty acids, betahydroxybutyric acid, nitric oxide and calcium values differed from the experimental groups to which feed additives was added compared to the control group (P<0.05), it was observed that they had no effect on serum malondialdehyde, phosphorus, total protein and glucose concentrations (P>0.05).

Keywords

• Chia
• Tushin sheep
• Probiotic
• Performance
• Rumen parameters
• Blood parameters

References

1. Abd El-Ghani AA. Infuence of diet supplementation with yeast culture (Saccharomyces cerevisiae) on performance of Zaraibi goats. Small Ruminant Research 2004; 52(3): 223-229.
2. Alagawany M., Nasr M., Al-Abdullatif A., Alhotan RA., Azzam MM., Reda FM. Impact of dietary cold- pressed chia oil on growth, blood chemistry, haematology, immunity and antioxidant status of growing Japanese quail. Italian Journal of Animal Science 2020; 19(1): 896-904.
3. Amin AB., Mao S. Influence of yeast on rumen fermentation, growth performance and quality of products in ruminants: A review. Animal Nutrition 2021; 7(1): 31-41.
4. Asad T., Mehmood S., Mahmud A., Basheer A., Saleem G., Jatoi AS., Hussain J., Husnain F, Younis M. Ameliorating effect of different anti-stressors on growth performance, and immunophysiological responses in heat stressed broilers chickens. Pakistan Veterinary Journal 2019; 39(2): 285-288.
5. Ayaşan T., Ayaşan Ş. İnsan ve hayvan beslenmesinde Chia (Salvia hispanica) Kullanılması. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2020; 3(1): 48-57.
6. Ayerza R., Coates W. Influence of chia on total fat, cholesterol, and fatty acid profile of Holstein cow's milk. Revista Científica de UCES 2006; 2, 39-48.
7. Ayerza R., Coates W., Lauria M. Chia seed (Salvia hispanica L.) as an x-3 fatty acid source for broilers: Influence on fatty acid composition, cholesterol and fat content of white and dark meats, growth performance, and sensory characteristics. Poultry Science 2002; 81(6): 826-837.
8. Cherif M., Valenti B., Abidi S., Luciano G., Mattioli S., Pauselli M., Bouzarraa I., Priolo A., Salem BH. Supplementation of Nigella sativa seeds to Barbarine lambs raised on low- or high-concentrate diets: Effects on meat fatty acid composition and oxidative stability. Meat Science 2018; 139, 134–141.

9. Didarkhah M., Vatandoos TM. The effect of probiotic and prebiotic supplements on growth performance, blood parameters and skeletal growth of Baluchi male lambs. Iranian journal of applied animal science 2021 12(4): 411-422.
10. Ding G., Chang Y., Zhao L., Zhou Z., Ren L., Meng Q. Effect of Saccharomyces cerevisiae on alfalfa nutrient degradation characteristics and rumen microbial populations of steers fed diets with different concentrate-to-forage ratios. Journal of Animal Science and Biotechnology 2014; 5(1): 1-9.
11. Ersahince AC., Kara K. Nutrient composition and in vitro digestion parameters of Jerusalem artichoke (Helianthus tuberosus L.) herbage at different maturity stages in horse and ruminant. Journal of Animal and Feed Sciences 2017; 26(3): 213-225.
12. Foksowicz-Flaczyk J., Wójtowski JA., Danków R., Mikołajczak P., Pikul J., Gryszczyńska A., Łowicki Z., Zajączek K., Stanisławski D. The effect of herbal feed additives in the diet of dairy goats on intestinal lactic acid bacteria (LAB) count. Animals 2022; 12(3), 255.
13. García-Diaz T., Braco AF., Jacovaci FA., Jobim CC., Bolson DC., Daniel JLP. Inclusion of live yeast and mannan-oligosaccharides in high grain-based diets for sheep: ruminal parameters, inflammatory response and rumen morphology. PLoS One 2018; 13(2): e0193313.
14. Guedes CM., Gonçalves D., Rodrigues MAM., Dias-da-Silva A. Effects of a Saccharomyces cerevisiae yeast on ruminal fermentation and fibre degradation of maize silages in cows. Animal Feed Science and Technology 2008; 145(1-4): 27-40.
15. Habeeb AAM., El-Tarabany AA. Effect of Nigella sativa or curcumin on daily body weight gain, feed intake and some physiological functions in growing Zaraibi goats during hot summer season. Journal of Radiation Research and Applied Sciences 2012; 5(2): 60-78.
16. Hassan A., Gado H., Anele UY., Berasain MAM., Salem AZM. Influence of dieatary probiotic inclusion on growth performance, nutrient utilization, ruminal fermentation activities and methane production in growing lambs. Animal Biotechnology 2020; 31(4): 365-372.
17. Hossain SA., Parnerkar S., Haque N., Gupta RS., Kumar D., Tyagi, AK. Influence of dietary supplementation of live yeast (Saccharomyces cerevisiae) on nutrient utilization, ruminal and biochemical profiles of Kankrej calves. International Journal of Applied Animal Sciences 2012; 1(1): 30-38.
18. Hučko B., Bampidis VA., Kodeš A., Christodoulou V., Mudřik Z., Poláková K., Plachý V. Rumen fermentation characteristics in pre-weaning calves receiving yeast culture supplements. Czech Journal of Animal Science 2009; 54(10), 435-442.
19. Jia P., Cui K., Ma T., Wan F., Dang W., Yang D., Wang Y., Guo B., Zhao L., Diao Q. Influence of dieatry supplementation with Bacillus licheniformis and Saccharomyces cerevesia as alternatives to monensin on growth performance, antioxidant, immunity, ruminal fermentation and microbial diversity of fattening lambs. Scientific Reports 2018; 8(1): 16712.
20. Kafilzadeh F., Payandeh S., Gómez-Cortés P., Ghadimi D., Schiavone A., Marín ALM. Effects of probiotic supplementation on milk production, blood metabolite profile and enzyme activities of ewes during lactation. Italian Journal of Animal Science 2019; 18(1), 134-139.
21. Khadem AA., Pahlavan M., Afzalzadeh A., Rezaeian M. Effect of live yeast Saccharomyces cerevisiae on fermentation parameters and microbial populations of rumen, total tract digestibility of diet nutrients and on the in situ degradability of alfalfa in Iranian Chall sheep. Pakistan Journal Of Biological Sciences 2007; 10(4): 590-597.
22. Krizova L., Richter M., Trinacty J., Ríha J., Kumprechtova D. The effect of feeding live yeast cultures on ruminal pH and redox potential in dry cows as continuously measured by new wireless devise. Czech Journal of Animal Science 2011; 56(1): 37-45.
23. Landers TF., Cohen B., Wittum TE., Larson EL. A review of antibiotic use in food animals: perspective, policy, and potential. Public Health Reporting 2012; 127(1): 4-22.
24. Lettat A., Noziere P., Silbergberg M., Morgavi DP., Berger C., Martin C. Rumen microbial and fermentation characteristics are affected differently by bacterial probiotic supplementation during induced lactic and subacute acidosis in sheep. BMC Microbiology 2012; 12, 142.
25. Li F., Zhang Z., Li X., Zhu B., Guo L., Li F., Weng X. Effect of duration of linseed diet supplementation before slaughter on the performances, meat fatty acid composition and rumen bacterial community of fattening lambs. Animal Feed Science and Technology 2020; 263, 114457.
26. Lillehoj H., Liu Y., Calsamiglia S., Fernandez-Miyakawa ME., Chi F., Cravens RL., Oh S., Gay CG. Phytochemicals as antibiotic alternatives to promote growth and enhance host health. Veterinary Research 2018; 49(1): 1-18.
27. Lopez-Soto MA., Vald´es-García YS., Plascencia A., Barreras A., Castro-P´erez BI., Estrada-Angulo A., Ríos FG., Gomez-V ´azquez´ A., Corona L., Zinn RA. Influence of feeding live yeast on microbial protein synthesis and nutrient digestibility in steers fed a steam-flaked corn-based diet. Acta Agriculturae Scandinavica, Section A–Animal Science 2013; 63(1): 39-46.
28. Lyons T., Boland T., Storey S., Doyle E. Linseed oil supplementation of lambs’ diet in early life leads to persistent changes in rumen microbiome structure. Frontiers in Microbiology 2017; 8, 1656.
29. Malekkhahi M., Tahmasbi AM., Naserian, AA., Danesh-Mesgaran M., Kleen JL., AlZahal O., Ghaffari MH. Effects of supplementation of active dried yeast and malate during sub-acute ruminal acidosis on rumen fermentation, microbial population, selected blood metabolites, and milk production in dairy cows. Animal Feed Science and Technology 2016; 213, 29-43.
30. Markowiak P., Slizewska K. The role of probiotics, prebiotics, and synbiotics in animal nutrition. Gut Pathogens 2018; 10(1): 21.
31. McAllister TA., Beauchemin KA., Alazzeh AY., Baah J., Teather RM., Stanford K. Review: The use of direct fed microbials to mitigate pathogens and enhance production in cattle. Canadian Journal of Animal Science 2011; 91(2): 193-211.
32. Meena MK., Joshi M., Nagar M. Effect of feeding herb (Allium sativum) and probiotic (Saccharomyces cerevisiae) alone or in combination with ground nut straw based complete feed on haemato- biochemical parameters of Sonadi sheep The Pharma Innovation Journal 2022; 10(6): 277-280.
33. Mendonça NBSN., Sobrane Filho ST., Oliveira DH, Lima EMC, Rosa PVE, Faria PB, Naves LP, Rodrigues PB. Dietary chia (Salvia hispanica L.) improves the nutritional quality of broiler meat. Asian-Australasian Journal of Animal Sciences 2020; 33(8): 1310-1322.
34. Nasr MAF., Mohammed H., Hassan RA., Swelum AA., Saadeldin IM. Does light intensity affect the behaviour, welfare, performance, meat quality, amino acid profile and egg quality of Japanese quails? Poultry Science 2019; 98(8): 3093-3102.
35. Niewold TA. The nonantibiotic anti-inflammatory effect of antimicrobial growth promoters, the real mode of action? A hypothesis. Poultry Science 2007; 86(4): 605-609.
36. NRC (National Research Council). Nutrient Requirements of Dairy Cattle. 7th ed. National Academy Press. 2001.
37. Opsi F., Fortina R., Tassone S., Bodas R., López S. Effects of inactivated and live cells of Saccharomyces cerevisiae on in vitro ruminal fermentation of diets with different forage: concentrate ratio. The Journal of Agricultural Science 2012; 150(2), 271-283.
38. Özbek T., Yeşilçubuk NŞ. Süper besin: Chia tohumu (Salvia hispanica L.). Beslenme ve Diyet Dergisi 2018; 46(1): 90-96.
39. Rasul M., Mehmod S., Ahmad S., Javid A., Mahmud A., Rehman A., Usman M., Hussain J., Ahmad M., Azhar M. Effects of different anti-stressors on growth, serum chemistry and meat quality attributes of Japanese. Brazilian Journal of Poultry Science 2019; 21(1): 1-10.
40. Sadarman, Febrina D., Yendraliza, Haq MS., Nurfitriani RA., Barkah NN., Sholikin MM., Yunilas, Qomariyah N., Jayanegara A., Solfaine R., Irawan A. Effect of dietary black cumin seed (Nigella sativa) on performance, immune status, and serum metabolites of small ruminants: A meta-analysis. Small Ruminant Research 2021; 204: 106521.
41. Sadiek A., Böhm J. Infuence of Pronifer® as a probiotic on the rumen fuid and blood parameters of sheep fed diferent roughage concentrate based diets. Wiener Tierarztliche Monatsschrift 2001; 88(1): 4-10.
42. Sajadian R., Seifi HA., Mohri M., Naserian AA., Farzaneh N. Variations of energy biochemical metabolites in periparturient dairy Saanen goats. Comparative Clinical Pathology 2013; 22(3): 449-456.
43. Salem AZM., Gado HM., Colombatto D., Eghandour MMY. Effect of exogenous enzymes on nutrient digestibility, ruminal fermentation and growth performance in beef steers. Livestock Science 2013;154(1-3): 69-73.
44. Schettino B., Vega S., Gutierrez R., Escobar A., Romeo J., Dominguez E. Fatty acid profile of goat milk in diets supplemented with chia seed (Salvia hispanica L.). Journal Dairy Science 2017; 100(8): 6256-6265.
45. Tripathi MK., Karim SA. Effect of yeast cultures supplementation on live weight change, rumen fermentation, ciliate protozoa population, microbial hydrolytic enzymes status and slaughtering performance of growing lamb. Livestock Science 2011; 135(1): 17-25.
46. Tripathi MK., Karim SA., Chaturvedi OH., Verma DL. Effect of different liquid cultures of live yeast strains on performance, ruminal fermentation and microbial protein synthesis in lambs. Journal of Animal Physiology and Animal Nutrition 2008; 92(6): 631-639.
47. Ueda K., Ferlay A., Chabrot J., Loor JJ., Chilliard Y, Doreau M. Effect of linseed oil supplementation on ruminal digestion in dairy cows fed diets with different forage: concentrate ratios. Journal of Dairy Science 2003; 86(12): 3999-4007.
48. Uribe JAR., Perez JIN., Kauil HC., Rubio GR., Alcocer CG. Extraction of oil from chia seeds with supercritical CO2. The Journal of Supercritical Fluids 2011; 56(2): 174-178.
49. Uyeno Y., Shigemori S., Shimosato T. Effects of probiotics/prebiotics on cattle health and productivity: minireview. Microbes and Environments 2015; 30(2): 126-132.
50. Wang Z., He Z., Beauchemin KA., Tang S., Zhou C., Han X., Wang M., Kang J., Odonga NE., Tan Z. Comparison of two live Bacillus species as feed additives for improving in vitro fermentation of cereal straws. Animal Science Journal 2016; 87(1): 27-36.
51. Xiao JX., Alugongo GM., Chung R., Dong SZ., Li SL., Yoon I., Wu ZH., Cao ZJ. Effects of Saccharomyces cerevisiae fermentation products on dairy calves: Ruminal fermentation, gastrointestinal morphology, and microbial community. Journal of Dairy Science 2016; 99(7): 5401-5412.
52. Zapata O., Cervantes A., Barreras A., Monge-Navarro F., Gonzalez-Vizcarra VM., Estrada-Angulo A., Urias-Estrada JD., Corona L., Zinn RA., Martinez-Alvarez IG. Effects of single and combined supplementation of probiotics and prebiotics on ruminal fermentation, ruminal bacteria and total tract digestion. Small Ruminant Research 2021; 204, 106538.
53. Zhu W., We Z., Xu N., Yang F., Yoon I., Chung Y., Liu J., Wang J. Effects of Saccharomyces cerevisiae fermentation products on performance and rumen fermentation and microbiota in dairy cows fed a diet containing low quality forage. Journal of Animal Science and Biotechnology 2017; 8(1): 1-9.