Sığır Lösemi Virüsü ile Enfekte Sığırlarda Telomer Uzunluğu ile Fizyolojik Parametreler Arasındaki İlişkinin Değerlendirilmesi

Abstract

Çalışmanın amacı, sığır lökozis virüsü ile enfekte sığırlarda göreceli telomer uzunlukları ile çeşitli fizyolojik parametreler arasındaki ilişkiyi araştırmak ve Türkiye'deki süt sığırlarında sığır lökozis virüsü enfeksiyonu ile ilişkili risk faktörlerini belirlemektir. Risk değerlendirmesi için yaş, doğum sayısı ve vücut kondisyon skoru olmak üzere üç değişken kullanılmıştır. Sığır lökozis virüsüne özgü antikorlar enzim bağlı immünosorbent testi kullanılarak tespit edilmiş ve göreceli telomer uzunlukları polimeraz zincir reaksiyonu ile ölçülmüştür. En yüksek seropozitiflik oranı (%39,13), vücut kondisyon skoru 3-3,5 olan ve 2-8 yaş arasındaki sığırlarda gözlenmiştir. Göreceli telomer uzunluğunun seropozitiflik, ileri yaş ve doğum sayısı ile pozitif korelasyonlu olduğu, seropozitiflik ile vücut kondisyon skoru arasında ise negatif korelasyon olduğu bulunmuştur. Sığır lökozis virüsü ile enfekte sığırlardaki göreceli telomer uzunlukları, enfekte olmayan sığırlara kıyasla anlamlı derecede daha kısa (P < 0,05) bulundu. Vücut kondisyon skoru grupları arasında en kısa göreceli telomer uzunlukları, vücut kondisyon skoru ≤ 2,5 olan sığır lökozis virüsü ile enfekte sığırlarda ve vücut kondisyon skoru 3-3,5 olan enfekte olmayan sığırlarda bulundu. Yaş gruplarına gelince, en kısa göreceli telomer uzunlukları, 8 yaşından büyük sığır lökozis virüsü ile enfekte sığırlarda (P < 0,05) ve aynı yaş grubundaki enfekte olmayan sığırlarda (P < 0,05) gözlendi. Sonuç olarak, sığır lökozis virüsü ile enfekte sığırlardaki göreceli telomer uzunluklarının, enfekte olmayan sığırlara kıyasla anlamlı derecede daha kısa olduğu bulundu (P < 0,05). En kısa göreceli telomer uzunlukları hem sığır lökozis virüsüyle enfekte olmuş hem de enfekte olmamış 8 yaş üstü sığırlarda kaydedildi (P < 0,05). Son olarak, sığır lökozis virüsü enfeksiyonu ile göreceli telomer uzunlukları arasındaki ilişki, yaş, doğum sayısı ve vücut kondisyon skoru gibi diğer faktörlerle birlikte, hastalık kontrolü ve önleme programları için yararlı olabileceği kanısına varıldı.

Keywords

• Yaş
• Vücut kondisyon skoru
• Sığır lökozis virüsü
• Doğum sayısı
• Göreceli telomer uzunluğu

Assessment of The Relationship Between Telomere Length and Physiological Parameters in Cattle Infected with Bovine Leukosis Virus

Abstract

The study aims to investigate the relationship between relative telomere lengths and various physiological parameters in cattle infected with bovine leukosis virus, as well as to identify the risk factors associated with cow-level bovine leukosis virus infection in dairy cattle in Türkiye. Three variables—age, parity, and body condition score—were used for risk assessment. Antibodies specific to bovine leukosis virus were detected using an enzyme-linked immunosorbent assay, and relative telomere lengths were measured by polymerase chain reaction. The highest seropositivity rate (39.13%) was observed in cattle with a body condition score of 3–3.5 and aged between 2–8 years. relative telomere length was found to be positively correlated with seropositivity, advanced age, and parity, while a negative correlation was observed between seropositivity and body condition score. Relative telomere lengths in bovine leukosis virus-infected cattle were significantly shorter (P < 0.05) than those in non-infected cattle. Among body condition score groups, the shortest relative telomere lengths were found in bovine leukosis virus-infected cattle with body condition score ≤ 2.5 and in non-infected cattle with body condition score 3–3.5. Regarding age groups, the shortest relative telomere lengths were observed in bovine leukosis virus-infected cattle older than 8 years (P < 0.05), as well as in non-infected cattle of the same age group (P < 0.05). In conclusion, relative telomere lengths in bovine leukosis virus-infected cattle were found to be significantly shorter than in non-infected cattle (P < 0.05). The shortest relative telomere lengths were recorded in both bovine leukosis virus-infected and non-infected cattle over 8 years of age (P < 0.05). Finally, the association between bovine leukosis virus infection and relative telomere lengths, along with other factors such as age, parity, and body condition score, is considered potentially useful for disease control and prevention programs.

Keywords

• Age
• Body condition score
• Bovine leukosis virus
• Parity
• Relative telomere length

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