Kurşun Nitrat ve Kadmiyum Klorürün Sıçan Akciğer Dokusu Üzerindeki Toksik Etkileri ve Sesamolün Koruyucu Rolü

Abstract

Yaygın çevre kirleticileri olarak kabul edilen kadmiyum ve kurşun, insan sağlığını olumsuz etkileyen toksik ağır metallerdir. Susam yağında bulunan sesamol, güçlü antioksidan özelliklere sahip fenolik bir bileşiktir. Bu çalışma, sıçan akciğer dokusunda kadmiyum ve/veya kurşunun neden olduğu toksisiteye karşı sesamolün koruyucu potansiyelini değerlendirmek amacıyla yürütülmüştür. Bu amaçla, Kurşun nitrat (LN), kadmiyum klorür (CdCl2), LN artı CdCl2, sesamol, LN artı sesamol, CdCl2 artı sesamol ve LN artı CdCl2 artı sesamol erkek sıçanlara 28 gün boyunca gavaj yoluyla uygulandı. Deneylerin sonunda akciğer dokusunda malondialdehit düzeyleri ve antioksidan enzimlerin (SOD, CAT, GPx and GST) aktiviteleri ile akciğer dokusundaki histopatolojik değişiklikler incelendi. LN ve/veya CdCl2 uygulamaları, akciğer dokusunda antioksidan enzim aktivitelerinde önemli bir düşüşe ve malondialdehit seviyelerinde artışa neden olmuştur. Ayrıca, sıçanların akciğer dokusuna ağır metal uygulamaları, interalveolar septumda kalınlaşma, alveolar dilatasyon, inflamatuar hücre infiltrasyonu ve bronşiyal epitel dejenerasyonu gibi histopatolojik değişikliklere neden olmuştur. Ağır metal uygulamalarına ek olarak sesamol uygulaması ise antioksidan enzim aktivitelerinde önemli bir artışa, malondialdehit seviyelerinde önemli bir düşüşe ve akciğer dokusundaki patolojik değişikliklerin hafifletilmesine yol açmıştır.

Keywords

• Kurşun nitrat
• Kadmiyum klorür
• Sesamol
• Akciğer
• Oksidatif stres
• Histopatoloji

Toxic Effects of Lead Nitrate and Cadmium Chloride on Rat Lung Tissue and The Protective Role of Sesamol

Abstract

Cadmium and lead, considered common environmental pollutants, are toxic heavy metals that adversely affect human health. Sesamol, found in sesame oil, is a phenolic compound with powerful antioxidant properties. This study was conducted to evaluate the protective potential of sesamol against toxicity induced by cadmium and/or lead in the lung tissue of rats. For this purpose, Lead nitrate (LN), cadmium chloride (CdCl2), LN plus CdCl2, sesamol, LN plus sesamol, CdCl2 plus sesamol, and LN plus CdCl2 plus sesamol were administered to male rats by gavage for 28 days. At the end of the experiments, malondialdehyde levels and activities of antioxidant enzymes (SOD, CAT, GPx and GST) in the lung tissue and pathological changes in the lung tissue were examined. LN and/or CdCl2 treatments caused a significant decrease in antioxidant enzyme activities and an increase in malondialdehyde levels in lung tissue. In addition, heavy metal applications in the lung tissue of rats caused histopathological changes such as thickening of the interalveolar septum, alveolar dilatation, inflammatory cell infiltration, and degeneration of the bronchial epithelium. The application of sesamol, in addition to heavy metal applications, led to a significant increase in antioxidant enzyme activities, a significant decrease in malondialdehyde levels, and an alleviation of pathological changes in lung tissue.

Keywords

• Cadmium chloride
• Lead nitrate
• Sesamol
• Lung
• Oxidative stress
• Histopathology

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