Farklı Böcek Taksonlarında Wolbachia Bakterisinin İncelenmesi

Year 2022, Volume: 5 Issue: 3, 1733 - 1743, 12.12.2022

Tayfun Kaya

https://doi.org/10.47495/okufbed.1123332

Abstract

Eklembacaklılar, popülasyon yoğunlukları ve dağılımları göz önüne alındığında en yaygın canlı türüdür. Ancak artan dünya nüfusu ve küresel ısınmaya bağlı kuraklık nedeniyle hem sağlık hem de tarımsal üretim açısından özellikle zararlı türlerle mücadelede çevre dostu ve etkili alternatif stratejiler geliştirmeye yönelik çalışmaları zorunlu kılmaktadır. Bu bağlamda Wolbachia, ev sahipleriyle olan ilişkileri nedeniyle umut vericidir. Ancak eklembacaklılarda simbiyotik yapı iklim, coğrafya ve ekosistem gibi farklılıklara göre değişmektedir. Bu çalışmada yaşam koşulları, konukçuları ve ekolojik nişleri bakımından farklı olan böcekler: Drosohphila melanosgaster (Diptera: Drosophiidae), Bemisia tabaci (Hemiptera: Aleyrodidae), Pulex irritans (Siphonaptera: Pulicidae), Eusomus ovulum (Coleoptera: Currioculionidae) ve Lariophagus distinguendus (Hymenoptera: Pteromalidae)'de Wolbachia enfeksiyonu incelenmiştir. Wolbachia, spesifik bir Wspec F/R primer seti ile tarandı ve elde edilen PCR ürünlerinin dizi verilerine göre tanımlandı. Wolbachia'nın çalışılan türlerde yaygın bir insidansa sahip olduğu gözlendi. Pire, sirke sinekleri ve E. ovulum’da A süpergrubu, parsitoid arı ve beyazsineklerde ise B süpergrubu Wolbachia bulunduğu tespit edildi. Bu çalışma, Türkiye'de E. ovulum'da Wolbachia varlığını bildiren ilk çalışma olup, burada sunulan verilerin yapılacak çalışmalara katkı sağlayacağı düşünülmektedir.

Keywords

Wolbachia, P. irritans, E. ovulum, L. distinguendus, D. melanosgaster, B. tabaci

Investigation of Wolbachia Bacteria in Different Insect Taxa

Abstract

Arthropods are the most common living species considering their population densities and distribution. However, due to the increasing world population and drought due to global warming, it is necessary to develop environmentally friendly and effective alternative strategies in terms of both health and agricultural production, especially in the fight against harmful species. In this context, Wolbachia is promising due to its relations with its hosts. However, the symbiotic structure in arthropods varies with differences such as climate, geography and ecosystem. In this study, Wolbachia infection was investigated in insects that differ in their living conditions, hosts and ecological niches: Drosohphila melanosgaster (Diptera: Drosophilidae), Bemisia tabaci (Hemiptera: Aleyrodidae), Pulex irritans (Siphonaptera: Pulicidae), Eusomus ovulum (Coleoptera: Currioculionidae) and Lariophagus distinguendus (Hymenoptera: Pteromalidae). Wolbachia was screened with a specific Wspec F/R primer set and identified according to the sequence data of the obtained PCR products. Wolbachia was observed to have a widespread incidence in the species studied. A supergroup Wolbachia was found in fleas, vinegar flies and E. ovulum, and B supergroup Wolbachia in parsitoid bees and whiteflies. This is the first study in Turkey to report the presence of Wolbachia in E. ovulum, and it is thought that the data presented here will contribute to future studies.

Keywords

Wolbachia, P. irritans, E. ovulum, L. distinguendus, D. melanosgaster, B. tabaci

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