Farklı Kitosan ve Polivinil Alkol Oranları Kullanılarak Hazırlanan Kriyojel Doku İskelelerinin Optimizasyon Çalışmaları

Year 2023, Volume: 6 Issue: 2, 1110 - 1121, 05.07.2023

Gülşah Gül , Didem Demir Karakuş , Nimet Bölgen

Abstract

Kriyojeller, monomerik veya polimerik başlatıcıların donmuş çözeltilerinden üretilen birbirine bağlı gözenekli matrislerle yapılandırılmış yapı iskeleleridir. Bu yapı iskeleleri, doku mühendisliği, yara örtü ve ilaç salım sistemleri dahil olmak üzere farklı biyomedikal alanlar için arzu edilen özelliklere sahip benzersiz adaylar olarak görülmektedir. Bu çalışmada, polivinil alkol (PVA) ve kitosan (CS) polimerleri kullanılarak PVA:CS kompozit kriyojelleri üretilmiştir. Farklı polimer oranlarında hazırlanan kriyojeller kimyasal yapı, morfoloji, gözeneklilik ve şişme oranı açısından değerlendirilmiştir. Kompozit kriyojellerin kimyasal yapısı Fourier dönüşümü kızılötesi spektroskopisi (FTIR) kullanılarak belirlenmiştir. Birbirine bağlı gözenek morfolojisi, Taramalı Elektron Mikroskobu (SEM) kullanılarak gözlemlenmiştir. Porozite ve şişme oranı değerleri, kriyojellerin ağırlık değişimi esas alınarak belirlenmiştir. Genel olarak tüm numuneler gözenekli bir yapı sergilemiş ve gözeneklilik dahil diğer özelliklerin doku iskelesi yapısındaki her bir polimerin oranına göre farklılık gösterdiği ortaya konmuştur.

Keywords

kitosan, polivinil alkol, doku iskelesi, kriyojel, doku mühendisliği

Supporting Institution

Mersin Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

2018-1-TP2-2733

Optimization Studies of Cryogel Scaffolds Prepared Using Different Chitosan and Polyvinyl Alcohol Ratios

Abstract

Cryogels are scaffolds structured with interconnected porous matrices produced from frozen solutions of monomeric or polymeric initiators. These scaffolds are seen as unique candidates with desirable properties for the different biomedical fields including tissue engineering, wound dressing, and drug delivery systems. In this study, polyvinyl alcohol (PVA) and chitosan (CS) were used to fabricate PVA:CS composite cryogels. Cryogels prepared at different polymer ratios were evaluated in terms of chemical structure, morphology, porosity, and swelling ratio. The chemical structure of composite cryogels was determined using Fourier-transform infrared spectroscopy (FTIR). The interconnected pore morphology was observed using Scanning Electron Microscopy (SEM). Porosity and swelling ratio values were determined based on the weight change of the cryogels. In general, all samples exhibited a porous structure, and it was revealed that porosity and other properties differ according to the ratio of each polymer in the scaffolds.

Keywords

chitosan, polyvinyl alcohol, scaffold, cryogel, tissue engineering

Project Number

2018-1-TP2-2733

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