Investigation of chelate and microbial fertilizer assisted phytoremediation efficiency of onion (Allium cepa L.) in lead contaminated soils

Year 2023, Volume: 6 Issue: Ek Sayı, 263 - 277, 20.12.2023

Aslıhan İpek Tanyıldız , Dudu Duygu Kılıç

Abstract

in recent years. Since lead is one of the most widely used elements in industry, it is detected in soil pollution. In recent years, phytoremediation has been preferred as an alternative method for the removal of soil pollution and phytoremediation protects the biological properties and physical structure of the soil. In this study, the effects of EDTA (ethylenediamine tetraacetic acid) and humic acid chelating agents and microbial fertilization on phytoremediation efficiency and plant growth parameters of Allium cepa L. (onion) in lead contaminated soils were investigated. Accordingly, it was determined that root TI values were higher in the pots where EDTA + Microbial Fertilizer combination was applied. In the pots where humic acid was applied, root TI values were higher. Again, leaf wet weight and dry weight TI values were higher in the pots where EDTA + Microbial Fertilizer combination was applied. It was determined that microbial fertilizer increased the tolerance index of the species. According to Pb values in A. cepa, it was found in root, bulb, leaf and total plant (28,93 mg kg-1, 36,16 mg kg-1, 262,56 mg kg-1, 327,65 mg kg-1) in pots with 5 mmol kg-1 EDTA addition, respectively. High accumulation (16.19 mg kg-1) was found at 10 mmol kg-1 in total plant in pots with humic acid addition. It was found that the plant tended to accumulate Pb more in its leaves. BCF values of EDTA and Humic Acid chelates were found to be greater than 1. BCF values of Allium cepa grown in chelate + microbial fertilizer combination were found to be less than 1. As a result, it can be said that the addition of microbial fertilizer decreased the Pb uptake of the species.

Keywords

Phytoremediation, Allium cepa L., Chelate, EDTA, Humic Acid., Microbial Fertilizer

Supporting Institution

Amasya Üniversitesi

Project Number

FMB-BAP 17-0246

Thanks

Amasya University BAP unit (FMB-BAP 17-0246) has supported this study

Kurşunla kirlenmiş topraklarda soğanın (Allium cepa L.) şelat ve mikrobiyal gübre destekli fitoremediasyon etkinliğinin araştırılması

Abstract

Ağır metal kirliliği son yıllarda artan çevre kirliliği başında gelmektedir. Kurşun sanayide en fazla kullanılan elementlerden bir olduğu için toprak kirliliğinde tespit edilmektedir. Toprak kirliliğinin giderilmesinde son yıllarda alternatif bir yöntem olarak fitoremediasyon tercih edilmektedir ve fitoremediasyon toprağın biyolojik özelliklerini ve fiziksel yapısını korumaktadır. Bu çalışmada, EDTA (Etilendiamin tetraasetik asit) ve Hümik Asit şelatlayıcı ajanlarının ve mikrobiyal gübrelemenin kurşunla kirlenmiş topraklarda Allium cepa L. (soğan)’ nin fitoremediasyon etkinliği ve bitki büyüme parametreleri üzerindeki etkileri araştırılmıştır. Buna göre EDTA +Mikrobiyal Gübre kombinasyonu uygulanan saksılarda kök Tİ değerlerinin daha yüksek olduğu tespit edilmiştir. Hümik asit uygulanan saksılarda ise kök Tİ değerleri daha yüksektir. Yine EDTA +Mikrobiyal Gübre kombinasyonu uygulanan saksılarda Yaprak yaş ağırlık ve kuru ağırlık Tİ değerleri yüksektir. Araştırmada mikrobiyal gübrenin türün tolerans indeksini arttırdığı tespit edilmiştir. A. cepa’da Pb değerlerlerine göre 5 mmol kg-1 EDTA ilaveli saksılarda sırası ile kök, bulb, yaprak ve toplam bitkide (28,93 mg kg-1, 36,16 mg kg-1, 262,56 mg kg-1 ,327,65 mg kg-1 ) bulunmuştur. Hümik asit ilaveli saksılarda toplam bitkide 10 mmol kg-1’da yüksek akümülasyon (16,19 mg kg-1) bulunmuştur. Bitkinin Pb ‘yi yaprağında daha çok biriktirme eğilimi gösterdiği tespit edilmiştir. EDTA ve Hümik Asit şelatlarında BCF değerlerinin 1’den büyük olduğu tespit edilmiştir. Şelat + mikrobiyal gübre kombinasyonunda yetiştirilen Allium cepa BCF değerlerinin 1’den küçük olduğu tespit edilmiştir. Sonuç olarak mikrobiyal gübre ilavesinin türün Pb alımını düşürdüğü söylenebilir.

Keywords

Fitoremediasyon, Allium cepa L., Şelat, EDTA, Hümik Asit

Project Number

FMB-BAP 17-0246

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