Aloe Vera Bitkisinden Yeşil Sentez Yöntemiyle Elde Edilen Gümüş Nanopartiküllerin Antimikrobiyal ve Antibiyofilm Etkilerinin Araştırılması

Year 2024, Volume: 7 Issue: 1, 166 - 176, 22.01.2024

Aybek Yiğit , Ayşe Karacalı Tunç , Büşra Merve Sarıtaş

https://doi.org/10.47495/okufbed.1279010

Cited By: 1

Abstract

Küresel olarak antibiyotiklerin yanlış kullanımı, dirençli bakteri suşlarının gelişmesine neden olmuştur. Aloe vera ekstraktından yeşil sentez ile elde edilen gümüş nanopartiküllerin Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii ve Staphylococcus aureus'a karşı antimikrobiyal ve antibiyofilm etkileri araştırıldı. Aloe vera bitkisinden gümüş nanopartiküllerin sentezi yeşil sentez yöntemi ile gerçekleştirilmiştir. Gümüş nanopartiküllerin karakterizasyonu UV-Vis Spektroskopi, FT-IR, SEM, TEM, XRD ile değerlendirildikten sonra patojenik mikroorganizmalar üzerindeki antimikrobiyal etkisi mikrodilüsyon yöntemi ile değerlendirilmiştir. Gümüş nanopartiküllerin Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii ve Staphylococcus aureus üzerindeki Minimum İnhibitör Konsantrasyon (MIC) değerleri (µg/mL) sırasıyla 32, 64, 32 ve 32 olarak bulundu. Bu çalışmada, biyofilm inhibisyon oranı kristal viyole yöntemi ile değerlendirilmiştir. Yeşil sentez gümüş nanopartiküller bakteriler üzerinde antibiyofilm etki göstermiştir.

Keywords

yeşil sentez,, antimikrobiyal etki, antibiyofilm etki, aloe vera özütü

Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis

Abstract

Misuse of antibiotics globally has resulted in the development of resistant bacterial strains. Antimicrobial and antibiofilm effects of silver nanoparticles obtained by green synthesis from Aloe vera extract against Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus aureus were investigated. The synthesis of silver nanoparticles from the aloe vera plant was carried out by the green synthesis method. After the characterization of silver nanoparticles was evaluated by UV-Vis Spectroscopy, FT-IR, SEM, TEM, XRD its antimicrobial effect on pathogenic microorganisms was evaluated by microdilution method. The Minimum Inhibitor Concentration (MIC) values (µg/mL) of silver nanoparticles on Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and Staphylococcus aureus were found to be 32, 64, 32 and 32, respectively. In this study, the biofilm inhibition rate was evaluated by the crystal violet method. Green synthesis silver nanoparticles showed antibiofilm effect on bacteria.

Keywords

green synthesis, antimicrobial effect, antibiofilm effect, aloe vera extract

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