Probiotics in Horse Nutrition

Year 2025, Volume: 8 Issue: 4, 1993 - 2005, 16.09.2025

Yavuzkan Paksoy , Ahmet Koluman

https://doi.org/10.47495/okufbed.1568373

Abstract

This paper investigates the potential for probiotics to transform horse-breeding techniques by improving gut health, immunological function, reproductive performance, and general horse well-being. The complex interaction between the gut microbiota and horse health is addressed, stressing the negative consequences of gut symbiosis. Probiotics are proposed as a viable alternative, providing several benefits to breeding efforts. The article investigates the particular effects of probiotics on mares, stallions, and offspring. There are many studies reporting that probiotics can increase pregnancy rates and foal development, as well as reduce infections in the reproductive organs. Promising probiotic strains are found, along with critical selection criteria. The significance of optimal dosing, administration, and integration with other management methods is highlighted. Finally, the analysis emphasizes the need for more research to fully realize the promise of probiotics in equestrian breeding. By embracing this new frontier, horse breeders may help to make the equine industry healthier and more prosperous.

Keywords

Foal development , Gut health , Horse breeding , Probiotics , Reproductive performance

At Beslemede Probiyotik Kullanımı

Abstract

Bu makale, probiyotiklerin bağırsak sağlığını, immünolojik fonksiyonunu, üreme performansını ve genel at refahını iyileştirerek at yetiştirme tekniklerini dönüştürme potansiyelini araştırmaktadır. Bağırsak mikrobiyotası ve at sağlığı arasındaki karmaşık etkileşim, bağırsak disbiyozunun olumsuz sonuçlarını vurgulayarak ele alır. Probiyotikler, yetiştirme faaliyetlerine çeşitli faydalar sağlayarak uygulanabilir bir alternatif olarak önerilmektedir. Makale, probiyotiklerin kısraklar, aygırlar ve yavrular üzerindeki özel etkilerini araştırmaktadır. Probiyotiklerin gebelik oranını ve tay gelişimini arttırmasının yanı sıra üreme organlarında meydana gelen enfeksiyonları azalttığını bildiren birçok araştırma vardır. Kritik seçim kriterleri ile birlikte umut verici probiyotik suşlar bulunur. Optimal dozlama, uygulama ve diğer yönetim metotlarıyla entegrasyonun önemi vurgulanmaktadır. Sonuç olarak, çalışma at yetiştiriciliğinde probiyotiklerin vaadini tam olarak anlamak için daha fazla araştırmaya ihtiyaç olduğunu vurgulamaktadır. Bu yeni yaklaşımı benimseyerek, at yetiştiricileri atçılık endüstrisinin daha sağlıklı ve daha müreffeh hale getirmeye yardımcı olabilir.

Keywords

At yetiştiriciliği , Bağırsak sağlığı , Probiyotikler , Tay gelişimi , Üreme performansı

References

• Adams VJ., LeBlanc N., Penell J. Results of a clinical trial showing changes to the faecal microbiome in racing thoroughbreds after feeding a nutritional supplement. Veterinary Sciences 2022; 10(1): 27. doi: 10.3390/vetsci10010027
• Angelika S. Probiotics and dietary supplements in horses: useful or not? In: Annual Forum of the American College of Veterinary Internal Medicine 2014; 1-3. Nashville.
• Arnold CE., Pilla R. What is the microbiota and what is its role in colic? Veterinary Clinics of North America-equine Practice 2023; 39(1): 381-397. doi: 10.1016/j.cveq.2023.03.004
• Barba M., Martínez-Boví R., Quereda JJ., Mocé ML., Plaza-Dávila M., Jiménez-Trigos E., García-Roselló E. Vaginal microbiota is stable throughout the estrous cycle in Arabian mares. Animals 2020; 10(11).
• Berreta A., Burbick CR., Alexander T., Kogan C., Kopper JJ. Microbial variability of commercial equine probiotics. Journal of Equine Veterinary Science 2021; 106 (s2): 103728.
• Berreta A., Kopper J. Equine probiotics-what are they, where are we and where do we need to go? Journal of Equine Veterinary Science 2022; 115, 104037.
• Cooke CG., Gibb Z., Harnett JE. The safety, tolerability and efficacy of probiotic bacteria for equine use. Journal of Equine Veterinary Science 2021; 99, 103407.
• Cooke CG. The microbiome and stallion fertility. (Doctoral Thesis), The University of Sydney, Faculty of Medicine and Health, School of Pharmacy, Sydney, New South Wales, Australia, 318. October 27, 2023. https://ses.library.usyd.edu.au/bitstream/handle/2123/31917/cooke_cg_thesis.pdf?sequence=1
• Cooke CG., Gibb Z., Grupen CG., Schemann K., Deshpande N., Harnett J. The ımpact of probiotics and prebiotics on the composition of the equine faecal and seminal microbiomes and sperm quality–a Pilot Study 2023; 1-25. Available at SSRN 4534385.
• Cooke CG., Gibb Z., Grupen CG., Harnett J. Prebiotics and synbiotics in equine health and disease: probiotics and synbiotics for horses. International Journal of Equine Science 2023; 2(2): 37-47.
• Cooke CG., Gibb Z., Grupen CG., Schemann K., Deshpande N., Harnett JE. Effect of probiotics and prebiotics on the composition of the equine fecal and seminal microbiomes and sperm quality: a pilot study. Journal of Equine Veterinary Science 2024; 135: 105032.
• De Rezende ASC., Trigo P., Lana ÂMQ., Santiago JM., Silva V., Montijano FC. Yeast as a feed additive for training horses. Ciência e Agrotecnologia 2012; 36: 354-362.
• Elghandour MMY., Tan ZL., Abu Hafsa SH., Adegbeye MJ., Greiner R., Ugbogu EA., Salem AZM. Saccharomyces cerevisiae as a probiotic feed additive to non and pseudo‐ruminant feeding: a review. Journal of Applied Microbiology 2020; 128(3): 658-674.
• Freccero F., Lanci A., Mariella J., Viciani E., Quercia S., Castagnetti A., Castagnetti, C. Changes in the Fecal microbiota associated with a broad-spectrum antimicrobial administration in hospitalized neonatal foals with probiotics supplementation. Animals 2021; 11(8): 2283.
• Ganda E., Chakrabarti A., Sardi MI., Tench M., Kozlowicz BK., Norton SA., Warren LK., Khafipour E. PSXV-11 A Postbiotic from saccharomyces cerevisiae fermentation improves microbiome robustness in young stress-challenged horses in training. Journal of Animal Science 2022; 15: 101. doi: 10.1093/jas/skac247.531
• Ganda E., Chakrabarti A., Sardi MI., Tench M., Kozlowicz BK., Norton SA., Warren LK., Khafipour E. Saccharomyces cerevisiae fermentation product improves robustness of equine gut microbiome upon stress. Frontiers in Veterinary Science 2023; 10: 1134092. doi: 10.3389/fvets.2023.1134092
• Goodman-Davis R., Figurska M., Cywinska A. Gut microbiota manipulation in foals—Naturopathic Diarrhea Management, or Unsubstantiated Folly? Pathogens 2021; 10(9): 1137.
• Gülhan T., Boynukara B. The use of probiotics in veterinary medicine. Doğanın Sesi 2018; 1(1): 43-48.
• Hamad G., Ombarak RA., Eskander M., Mehany T., Anees FR., Elfayoumy RA., Omar SA., Lorenzo JM., Abou-Alella SAE. Detection and inhibition of Clostridium botulinum in some Egyptian fish products by probiotics cell-free supernatants as bio-preservation agents. LWT 2022; 163: 113603. doi: 10.1016/j.lwt.2022.113603
• Hu G., Do DN., Gray J., Miar Y. Selection for favorable health traits: a potential approach to cope with diseases in farm animals. Animals 2020; 10(9): 1717.
• Ishizaka S., Matsuda A., Amagai Y., Oida K., Jang H., Ueda Y., Takai M., Tanaka A., Matsuda H. Oral administration of fermented probiotics improves the condition of feces in adult horses. Journal of Equine Science 2014; 25(4): 65-72.
• Ivannikova R., Bessarabova E., Smirnova E., Lunegov AM. Effect of probiotic feed additive on physiological and biochemical parameters of horses. EDP Sciences, E3S Web of Conferences 2023; 431(3): 01044.
• Jacobs RD., Grum D., Trible B., Ayala DI., Karnezos TP., Gordon ME. Oral probiotics administration attenuates postexercise inflammation in horses. Translational Animal Science 2024; 8, 124.
• Jones E. Characterization of the equine microbiome during late gestation and the early postpartum period, and at various times during the estrous cycle in mares being bred with raw or extended semen (Doctoral Thesis), Kansas State University, Department of Animal Sciences and Industry College of Agriculture, Manhattan, Kansas, 2019.
• Kaya ZK., Paksoy Y., Koluman N. The effect of age, gender and feeding method on some body measurements in pony horses. Journal of Animal Production 2024; 65(1): 59-65.
• Kritas SK. Probiotics and prebiotics for the health of pigs and horses. Probiotics and Prebiotics in Animal Health and Food Safety 2018; 109-126.
• Lagounova M. The use of dietary probiotics in horses: Microbial activity during antibiotic treatment and bacterial characterization (Doctoral Thesis), The University of Guelph, In partial fulfilment of requirements for the degree of Master of Science in Animal Biosciences, Guelph, Ontario, Canada, 2022.
• Lambo MT., Chang X., Liu D. The recent trend in the use of multistrain probiotics in livestock production: An overview. Animals 2021; 11(10): 2805.
• Lauková A., Styková E., Kubašová I., Strompfová V., Gancarčíková S., Plachá I., Simonová MP. Enterocin M-producing Enterococcus faecium CCM 8558 demonstrating probiotic properties in horses. Probiotics and Antimicrobial Proteins 2020; 12: 1555-1561.
• Lauková A., Micenková L., Kubašová I., Bino E., Kandričáková A., Plachá I., Simonová MP. Microbiota, phagocytic activity, biochemical parameters and parasite control in horses with application of autochthonous, Bacteriocin-Producing, Probiotic Strain Enterococcus faecium EF 412. Probiotics and Antimicrobial Proteins 2023; 15(1): 139-148.
• Li C., Li X., Guo R., Ni W., Liu K., Liu Z., Dai J., Xu Y., Abduriyim S., Wu Z., Zeng Y., Lei B., Zhang Y., Wang Y., Zeng W., Zhang Q., Chen C., Qiao J., Liu C., Hu S. Expanded catalogue of metagenome-assembled genomes reveals resistome characteristics and athletic performance-associated microbes in horse. Microbiome, 2023; 11: 7. doi: 10.1186/s40168-022-01448-z
• MacNicol JL., Renwick S., Ganobis CM., Allen-Vercoe E., Weese JS., Pearson W. The influence of a probiotic/prebiotic supplement on microbial and metabolic parameters of equine cecal fluid or fecal slurry in vitro. Journal of Animal Science 2023; 101: skad034. doi: 10.1093/jas/skad034
• McGilloway M., Manley S., Aho A., Heeringa KN., Whitacre L., Lou Y., Squires EJ., Pearson W. Dietary fermentation product of aspergillus oryzae prevents increases in gastrointestinal permeability (‘Leaky Gut’) in horses undergoing combined transport and exercise. Animals 2023; 13(5): 951. doi: 10.3390/ani13050951
• Malaluang P., Wilén E., Frosth S., Lindahl J., Hansson I., Morrell JM. Vaginal bacteria in mares and the occurrence of antimicrobial resistance. Microorganisms 2022; 10(11): 2204.
• Malaluang P., Åkerholm T., Nyman G., Lindahl J., Hansson I., Morrell JM. Bacteria in the healthy equine vagina during the estrous cycle. Theriogenology 2024; 213: 11-18.
• Massacci FR., Clark A., Ruet A., Lansade L., Costa M., Mach N. Inter‐breed diversity and temporal dynamics of the faecal microbiota in healthy horses. Journal of Animal Breeding and Genetics 2020; 137(1): 103-120.
• Mienaltowski MJ., Callahan M., De La Torre U., Maga EA. Comparing microbiotas of foals and their mares’ milk in the first two weeks after birth. BMC Veterinary Research 2024; 20(1): 17.
• Mirzaei A., Razavi SA., Babazadeh D., Laven R., Saeed M. Roles of probiotics in farm animals: A review. Farm Animal Health and Nutrition 2022; 1(1): 17-25.
• Munir A., Javed GA., Javed S., Arshad N. Levilactobacillus brevis from carnivores can ameliorate hypercholesterolemia: in vitro and in vivo mechanistic evidence. Journal of Applied Microbiology 2022; 133(3): 1725–1742. doi: 10.1111/jam.15678
• Park T., Yoon J., Yun YM., Unno T. Comparison of the fecal microbiota with high- and low performance race horses. Journal of Animal Science and Technology 2023; doi: 10.5187/jast.2023.e45
• Perricone V., Sandrini S., Irshad N., Comi M., Lecchi C., Savoini G., Agazzi A. The role of yeast saccharomyces cerevisiae in supporting gut health in horses: An updated review on its effects on digestibility and intestinal and fecal microbiota. Animals 2022; 12(24): 3475. doi: 10.3390/ani12243475
• Schoster A., Weese JS., Guardabassi L. Probiotic use in horses–what is the evidence for their clinical efficacy? Journal of Veterinary Internal Medicine 2014; 28(6): 1640-1652.
• Schoster A., Guardabassi L., Staempfli HR., Abrahams M., Jalali M., Weese JS. The longitudinal effect of a multi‐strain probiotic on the intestinal bacterial microbiota of neonatal foals. Equine Veterinary Journal 2016; 48(6): 689-696.
• Shi J., Zhao G., Huang X., Li X., Ma Y., Yang K. Effects of lactobacillus rhamnosus supplementation on growth performance, immune function, and antioxidant capacity of newborn foals. Journal of Equine Veterinary Science 2023; 129: 104501.
• Silva JA., Castañares M., Mouguelar H., Valenciano JA., Pellegrino MS. Isolation of lactic acid bacteria from the reproductive tract of mares as potentially beneficial strains to prevent equine endometritis. Veterinary Research Communications 2024; 48(3): 1353-1366.
• Tanabe S., Suzuki T., Wasano Y., Nakajima F., Kawasaki H., Tsuda T., Morita H. Anti-inflammatory and intestinal barrier–protective activities of commensal lactobacilli and bifidobacteria in thoroughbreds: role of probiotics in diarrhea prevention in neonatal thoroughbreds. Journal of Equine Science 2014; 25(2): 37-43.
• Weese JS., Sharif S., Rodriguez‐Palacios A. Probiotics in veterinary medicine. Therapeutic Microbiology: Probiotics and Related Strategies 2008; 26: 341-356.
• Wilmink JM., Ladefoged S., Jongbloets A., Vernooij JC. The evaluation of the effect of probiotics on the healing of equine distal limb wounds. PloS one 2020; 15(7): e0236761.
• Zaib S., Hayat A., Khan I. Probiotics and their beneficial health effects. Mini-reviews in Medicinal Chemistry 2024; 24(1): 110-125.