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Determination of Plum pox virus, the causal agent of Sharka Disease, in Samsun Province

Year 2015, Volume: 30 Issue: 3, 227 - 235, 08.12.2015
https://doi.org/10.7161/anajas.2015.30.3.227-235

Abstract

Plum pox virus (PPV) is one of the most destructive and serious viral agent of stone fruits such as plum, peach, nectarine, apricot, almond, sweet and sour cherries. PPV strains have been classified into at least eight sub-groups. PPV has been detected in some orchards in different geographical regions of Turkey including Marmara, Central Anatolia, Aegean and Mediterranean, and the strains of PPV and their distributions have been determined. To date, there is no report on the presence of PPV in the Black Sea Region. PPV-like fruit and leaf symptoms were noticed in four stone fruit orchards in Canik district of Samsun province in July 2013, and a total of 22 samples were taken from nine apricot, six nectarine, four plum and three peach trees. Only one plum and two apricot trees were found to be infected by DAS-ELISA using virus-spesific polyclonal antiserum and RT-PCR method using PPV-specific primers. The partial nucleotide sequences of coat protein (CP) region of plum and apricot isolates were obtained. Nucleotide BLAST analysis showed that plum and apricot isolates had the highest (99%) sequence similarity at nucleotide level with PPV-AbTk isolate (PPV-T strain) found on apricot trees in Ankara province and the PPV Abricotier Turquie isolate. This study revealed that the occurrence and distribution of PPV infection in stone fruit orchards needs to be investigated in the Black Sea Region in detail.

References

  • Akbaş, B., Değirmenci, K., Çiftçi, O., Kaya, A., Yurtmen, M., Uzunoğulları, N., Çelik, N., Türkölmez, Ş. 2011. Update on Plum pox virus distribution in Turkey, Phytopathol. Mediterr., 50, 75−83, 2011.
  • Azeri, T. 1994. Detection of Virus Diseases of Stone Fruits in Agean Region of Turkiye. 9th Congress of Mediterranean Phytopathological Union, Kuşadası, Aydın, Türkiye. 511-513 p.
  • Baumgartnerova, H. 1996. First findings of Plum pox virus in wallnut trees (Juglans regia L.). Acta Virologica, 40: 59-60.
  • Berger, P., Barnett, O.W., Brunt, A.A., Colinet, D., Edwardson, 1.R., Hammond, 1., Hill, 1.H., Jordan, R.L., Kashwazaki, S., Makkouk, K., Morales, F.1., Rybicki, E., Spence, N., Ohki, S.T., Uyeda, I., van Zaayen, A. and Vetten, R.I. 2000. Family Potyviridae. In: Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses. Springer-Verlag, NY. pp703-724.
  • Brunt, A. A., K. Crabtree, M. J. Dallwitzitz, A. J. Gibbs, L. Watson. 1996. Viruses of plants. CAB International, 1484 pp.
  • Caglayan, K. 2006. Plum pox virus (PPV) in Turkey. Bulletin OEPP/EPPO Bulletin 36: 216.
  • Candresse, T., Cambra, M., Dallot, S., Lanneau, M., Asensio, M., Gorris, M. T., Revers, F., Macquaire, G., Olmos, A., Boscia, D., Quiot, J. B., Dunez, J. 1998. Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of izolates belonging to the D and M serotypes of Plum pox potyvirus. Phytopathology, 88: 198-204.
  • Candresse T., Cambra, M. 2006. Plum pox virus strains or types: historical perspective and current status. EPPO Bulletin 36, 239–246.
  • Candresse, T., Svanella-Dumas, L., Gentit, P., Çağlayan, K., Çevik, B. 2007. First report of the presence of Plum pox virus Rec strain in Turkey. Plant Disease 91, 331.
  • Ceylan, A., Gürcan, K., Akbulut, M., Ghaderi, M. 2014. Kayseri’de yüksek şarka enfeksiyonu. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 30 (2): 80-85.
  • Chirkov, S., Ivanov, P., Sheveleva, A. 2013. Detection and partial molecular characterization of atypical Plum pox virus isolates from naturally infected sour cherry. Archives of Virology, 158(6), 1383-1387.
  • Crescenzi, A., D’aquino, L., Comes, S., Nuzzaci, M., And Piazzolla, P. 1997. Charecterisation of the sweet cherry ısolate of Plum pox potyvirus, Plant Disease. 81.711-714.
  • Clark, M.R., Adams, A. M., 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. General Virology, 34: 475-483.
  • Çelik, N., Topkaya Kütük, B. 2013. Antalya ilinde şarka virüs hastalığının belirlenmesi. Derim, 30 (2):1-10.
  • Çıtır, A., İlbağı, H. 2008. Serological identification of some important viruses on fruit trees and bushes in Tekirdağ province of Turkey, Proceedings of the Twentieth International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops, Fruit Tree Diseases’’, Antalya, Turkey. ActaHorticulturae 781, 103-106.
  • Dunez, J. 1986. Preliminary observations on virus and virus like diseases of stone fruit trees in Mediterranean and near east countries. FAO Plant Protection Bulletin. 34 :43-48.
  • Elibüyük, İ.Ö., Erdiller, G. 1991. Ankara ilinde kayısı, erik ve şeftali ağaçlarında görülen şarka hastalığının yayılış alanlarının tespiti ve tanısı üzerinde araştırmalar. 6. Türkiye Fitopatoloji Kongresi. 7-11 Ekim 1991, İzmir. s. 411-414.
  • Elibüyük, İ. Ö. 2003. Natural spread of Plum pox virus in Ankara, Turkey. J. Phytopathology, 151 :617-619.
  • Elibüyük I.Ö. 2004. Current situation of sharka disease in Ankara, Turkey. Phytoparasitica, 32(4), 417–420.
  • Erdiller, G. 1988. Investigation on the causes of fruit droping of apricot and plum trees in Ankara province. Journal of Turkish Phytopathology, 17(3): 98.
  • Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17 (6): 368–376.
  • Garcia, J. A., Glasa, M., Cambra, M. Candresse, T. 2014. Plum pox virus and sharka: a model potyvirus and a major disease. Molecular Plant Pathology, 15(3): 226–241.
  • Glasa, M., Candresse, T. 2005. Partial sequence analysis of an atypical Turkish isolate provides further information on the evolutionary history of Plum pox virus (PPV). Virus Research, 108, 199–206.
  • Glasa, M., Paunovic, S., Jevremovic, D., Myrta, A., Pittnerová, S., Candresse, T. 2005. Analysis of recombinant Plum pox virus (PPV) isolates from Serbia confirms genetic homogeneity and supports a regional origin for the PPV-Rec subgroup. Arch. Virol. 150, 2051–2060.
  • Glasa, M., Palkovics, L., Komínek, P., Labonne, G., Pittnerová, S., Kúdela, O., Candresse, T., Šubr, Z. 2004. Geographically and temporally distant natural recombinant isolates of plum pox virus (PPV) are genetically very similar and form a unique PPV subgroup, J. Genet. Virol., 85, 2671–2681.
  • Glasa, M., Prikhodko, Y., Predajna, L., Nagyova, A., Shneyder, Y., Zhivaeva, T., Subr, Z., Cambra, M., Candresse, T. 2013. Characterization of sour cherry isolates of Plum pox virus from the Volga Basin in Russia reveals a new cherry strain of the virus. Phytopathology, 103(9), 972-979.
  • Gümüs, M., Paylan, I.C., Matic, S., Myrta, A., Sipahioglu, H.M., Erkan, S. 2007. Occurrence and distribution of stone fruit viruses and viroids in commercial plantings of Prunus species in western Anatolia, Turkey, Journal of Plant Pathology, 89, 265–268.
  • Gürcan, K., Öcal, N., Yılmaz, K.U., Ullah, S., Erdoğan, A., Zengin, Y. 2015. Evaluation of Turkish apricot germplasm using SSR markers: Genetic diversity assessment and search for Plum pox virus resistance alleles. Scientia Horticulturae, 193: 155–164.
  • Ilardi, V., Tavazza, M. 2015. Biotechnological strategies and tools for Plum pox virus resistance: trans-, intra-, cis-genesis, and beyond. Frontiers in Plant Science, 6: 379.
  • İlbağı, H., Cıtır, A., Bostan, H. 2008. Prunus spinosa L. A natural wild host of some important fruit viruses in Tekirdağ, Turkey, Proceedings of the Twentieth International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops, Fruit Tree Diseases, Antalya, Turkey, Acta Horticulturae, 781, 33-36.
  • James, D., Varga, A. 2005. Nucleotide sequence analysis of Plum pox virüs isolate W3174: Evidence of a new strain, Virus Research, 110, 143–150.
  • Kalashayan, A., Bilkey, N. D., Verderevskaya, T.D., Rubina, E. V. 1994. Plum Pox Potyvirus on sour Cherry in Moldova. EPPO Bulletin 24: 645-649.
  • Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2): 111–120.
  • Kurçman S. 1973. Nachweis des sharka-virus an aprikosen und pflaumenbaumen aumenbaumen in Ankara. Journal of Turkish Phytopathology 2, 124–129.
  • Koç, G., Baloğlu, S. 2006. First report of sharka in the Çukurova region of Turkey, Journal of Plant Pathology, 88(3 suppl.), 68.
  • Lopez-Moya J. J., Fernandez-Fernandez, M. R., Cambra, M., Garcia, J. A. 2000. Biotechnological Aspects of Plum pox virus. J. Biotech. 76: 121-136.
  • Myrta, A., Varga, A., James, D. 2006. The complete genome sequence of an El Amar isolate of plum pox virus (PPV) and its phylogenetic relationship to other PPV strains, Archives of Virology, 151, 1189–1198.
  • Nemchinov, L., Hadidi, A., Maiss, E., Cambra, M., Candresse, T., Damsteegt, V. 1996. Sour cherry strain of plum pox potyvirus (PPV): molecular and serological evidence for a new subgroup of PPV strains, Phytopathology, 86, 1215–1221.
  • Ogawa M. J., Zehr, I. E., Bırd, W. G., Ritchie, F. D., Uriu, K., Uyemoto, K.J. 1995. Compendium of stone fruit diseases, Page: 69, The American Phytopathological Society Press.
  • Olmos, A., Cambra, M., Dasi, M.A., Candresse, T., Esteban, O., Gorris, M.T. & Asensio, M. 1997. Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA. Journal of Virological Methods, 68: 127–137.
  • Palkovics, L., Burgyán, J., Balázs, E. 1993. Comparative sequence analysis of four complete primary structures of plum pox virus strains, Virus Genes, 7, 339–347.
  • Scorza, R., Callahan, A., Dardick, C., Ravelonandro, M., Polak, J., Malinowski, T., Zagrai, I., Cambra, M., Kamenova, I. 2013. Genetic engineering of Plum pox virus resistance: ‘HoneySweet’ plum—from concept to product. Plant Cell Tiss. Organ. Cult., 115, 1–12.
  • Saitou, N., Nei, M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4:406-425.
  • Sertkaya, G., Ulubaş, Ç., Çağlayan, K.. 2003. Detection and characterization of Plum pox potyvirus (PPV) by DAS-ELISA and RTPCR/ RFLP analysis in Turkey, Turkish Journal of Agriculture and Forestry TÜBİTAK. Vol. 27, S.213-220.
  • Şahtiyancı S. 1969. Virus de la sharka chez le prunier. Bulletin Phytosanitaire FAO 17, 69.
  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. 2013. MEGA 6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30: 2725-2729.
  • Thompson J.D, Higgins, D.G., Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 4673-80.
  • Ulubaş Serçe, Ç., Candresse, T., Svanella-Dumas, L., Krizbai, L., Gazel, M., Çağlayan, K.. 2009. Further characterization of a new recombinant group of Plum pox virus isolates, PPV-T, found in the Ankara province of Turkey. Virus Research, 142: 121–126.
  • Ulubaş Serçe, Ç., Gazel, M., Çağlayan, K. 2011. Plum pox virus streynlerinin Türkiye’ deki Dağılımı (Distribution of Plum pox virus strains in Turkey), Türkiye IV. Bitki Koruma Kongresi Bildirileri, Kahramanmaraş s. 72.
  • Wetzel, T., Candresse, T., Ravelonandro, M., Dunez, J. 1991. A polymerase chain reaction assay adapted to plum pox potyvirus detection. Journal of Virological Methods, 33: 355–365.
  • Wijkamp, I., Jan Van Der Gaag, D. 2011. Pest risk analyses for Plum pox virus (PPV). – Plant Protect. Serv. pp. 57, Wageningen
  • Yürektürk, M. 1984. Marmara Bölgesinde Sert Çekirdekli Meyvelerde Görülen Sharka Hastalığı Üzerinde Araştırmalar. Atatürk Bahçe Kültürleri Araştırma Enstitüsü Yayınları, 37 s.Anonymous, 1994. FAO, Water Quality for Agriculture. Irrigation and Drainage Paper, No:29 Rome.

Samsun ilinde sert çekirdekli meyve türlerinde Şarka hastalığı etmeninin (Plum pox virus) belirlenmesi

Year 2015, Volume: 30 Issue: 3, 227 - 235, 08.12.2015
https://doi.org/10.7161/anajas.2015.30.3.227-235

Abstract

Plum pox virus (PPV), erik, şeftali, nektarin, kayısı, badem, kiraz ve vişne gibi sert çekirdekli meyve ağaçlarının en önemli viral etmenlerinden birisidir. PPV ırkları günümüzde en az 8 alt grupta toplanmıştır. Türkiye’de bugüne kadar yapılan çalışmalarda Marmara, İç Anadolu, Ege ve Akdeniz Bölgelerinde PPV’nin bulaşık olduğu bahçeler tespit edilmiş, virüsün ırk düzeyinde tanısı yapılmış ve dağılımı belirlenmiştir. Karadeniz Bölgesi’nde ise şu ana kadar sert çekirdekli meyve üretimi yapılan alanlarda Şarka hastalığının varlığına dair bir kayda rastlanmamıştır. Bu çalışmada, Samsun ili Canik ilçesinde 2013 yılı Temmuz ayında PPV-benzeri yaprak ve meyve simptomları gösteren 4 bahçe belirlenmiş ve 9 kayısı, 6 nektarin, 4 erik ve 3 şeftali ağacından toplam 22 örnek test edilmek üzere alınmıştır. PPV’ye spesifik antiserum kullanılarak DAS-ELISA ve PPV’ye spesifik primerler kullanılarak RT-PCR yöntemleri ile örneklerin test edilmesi sonucunda, iki kayısı ağacı ve bir erik ağacının PPV ile enfekteli olduğu belirlenmiştir. Kayısı ve erik izolatlarının kısmi kılıf protein (CP) bölgesi nükleotid dizileri elde edilmiş ve nükleotid BLAST analizine göre en yüksek benzerliği (% 99) Ankara’da kayısı ağacından izole edilen AbTk izolatı (PPV-T strain) ve yine Türkiye’den elde edilen Abricotier Turquie izolatı ile gösterdiği belirlenmiştir. Bu çalışma, Karadeniz Bölgesi’nde Samsun ilinin yanısıra diğer illerde de sert çekirdekli meyve bahçelerinin PPV ile bulaşıklık durumunun detaylı incelenmesinin gerekliliğini ortaya koymuştur.

References

  • Akbaş, B., Değirmenci, K., Çiftçi, O., Kaya, A., Yurtmen, M., Uzunoğulları, N., Çelik, N., Türkölmez, Ş. 2011. Update on Plum pox virus distribution in Turkey, Phytopathol. Mediterr., 50, 75−83, 2011.
  • Azeri, T. 1994. Detection of Virus Diseases of Stone Fruits in Agean Region of Turkiye. 9th Congress of Mediterranean Phytopathological Union, Kuşadası, Aydın, Türkiye. 511-513 p.
  • Baumgartnerova, H. 1996. First findings of Plum pox virus in wallnut trees (Juglans regia L.). Acta Virologica, 40: 59-60.
  • Berger, P., Barnett, O.W., Brunt, A.A., Colinet, D., Edwardson, 1.R., Hammond, 1., Hill, 1.H., Jordan, R.L., Kashwazaki, S., Makkouk, K., Morales, F.1., Rybicki, E., Spence, N., Ohki, S.T., Uyeda, I., van Zaayen, A. and Vetten, R.I. 2000. Family Potyviridae. In: Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses. Springer-Verlag, NY. pp703-724.
  • Brunt, A. A., K. Crabtree, M. J. Dallwitzitz, A. J. Gibbs, L. Watson. 1996. Viruses of plants. CAB International, 1484 pp.
  • Caglayan, K. 2006. Plum pox virus (PPV) in Turkey. Bulletin OEPP/EPPO Bulletin 36: 216.
  • Candresse, T., Cambra, M., Dallot, S., Lanneau, M., Asensio, M., Gorris, M. T., Revers, F., Macquaire, G., Olmos, A., Boscia, D., Quiot, J. B., Dunez, J. 1998. Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of izolates belonging to the D and M serotypes of Plum pox potyvirus. Phytopathology, 88: 198-204.
  • Candresse T., Cambra, M. 2006. Plum pox virus strains or types: historical perspective and current status. EPPO Bulletin 36, 239–246.
  • Candresse, T., Svanella-Dumas, L., Gentit, P., Çağlayan, K., Çevik, B. 2007. First report of the presence of Plum pox virus Rec strain in Turkey. Plant Disease 91, 331.
  • Ceylan, A., Gürcan, K., Akbulut, M., Ghaderi, M. 2014. Kayseri’de yüksek şarka enfeksiyonu. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 30 (2): 80-85.
  • Chirkov, S., Ivanov, P., Sheveleva, A. 2013. Detection and partial molecular characterization of atypical Plum pox virus isolates from naturally infected sour cherry. Archives of Virology, 158(6), 1383-1387.
  • Crescenzi, A., D’aquino, L., Comes, S., Nuzzaci, M., And Piazzolla, P. 1997. Charecterisation of the sweet cherry ısolate of Plum pox potyvirus, Plant Disease. 81.711-714.
  • Clark, M.R., Adams, A. M., 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. General Virology, 34: 475-483.
  • Çelik, N., Topkaya Kütük, B. 2013. Antalya ilinde şarka virüs hastalığının belirlenmesi. Derim, 30 (2):1-10.
  • Çıtır, A., İlbağı, H. 2008. Serological identification of some important viruses on fruit trees and bushes in Tekirdağ province of Turkey, Proceedings of the Twentieth International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops, Fruit Tree Diseases’’, Antalya, Turkey. ActaHorticulturae 781, 103-106.
  • Dunez, J. 1986. Preliminary observations on virus and virus like diseases of stone fruit trees in Mediterranean and near east countries. FAO Plant Protection Bulletin. 34 :43-48.
  • Elibüyük, İ.Ö., Erdiller, G. 1991. Ankara ilinde kayısı, erik ve şeftali ağaçlarında görülen şarka hastalığının yayılış alanlarının tespiti ve tanısı üzerinde araştırmalar. 6. Türkiye Fitopatoloji Kongresi. 7-11 Ekim 1991, İzmir. s. 411-414.
  • Elibüyük, İ. Ö. 2003. Natural spread of Plum pox virus in Ankara, Turkey. J. Phytopathology, 151 :617-619.
  • Elibüyük I.Ö. 2004. Current situation of sharka disease in Ankara, Turkey. Phytoparasitica, 32(4), 417–420.
  • Erdiller, G. 1988. Investigation on the causes of fruit droping of apricot and plum trees in Ankara province. Journal of Turkish Phytopathology, 17(3): 98.
  • Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17 (6): 368–376.
  • Garcia, J. A., Glasa, M., Cambra, M. Candresse, T. 2014. Plum pox virus and sharka: a model potyvirus and a major disease. Molecular Plant Pathology, 15(3): 226–241.
  • Glasa, M., Candresse, T. 2005. Partial sequence analysis of an atypical Turkish isolate provides further information on the evolutionary history of Plum pox virus (PPV). Virus Research, 108, 199–206.
  • Glasa, M., Paunovic, S., Jevremovic, D., Myrta, A., Pittnerová, S., Candresse, T. 2005. Analysis of recombinant Plum pox virus (PPV) isolates from Serbia confirms genetic homogeneity and supports a regional origin for the PPV-Rec subgroup. Arch. Virol. 150, 2051–2060.
  • Glasa, M., Palkovics, L., Komínek, P., Labonne, G., Pittnerová, S., Kúdela, O., Candresse, T., Šubr, Z. 2004. Geographically and temporally distant natural recombinant isolates of plum pox virus (PPV) are genetically very similar and form a unique PPV subgroup, J. Genet. Virol., 85, 2671–2681.
  • Glasa, M., Prikhodko, Y., Predajna, L., Nagyova, A., Shneyder, Y., Zhivaeva, T., Subr, Z., Cambra, M., Candresse, T. 2013. Characterization of sour cherry isolates of Plum pox virus from the Volga Basin in Russia reveals a new cherry strain of the virus. Phytopathology, 103(9), 972-979.
  • Gümüs, M., Paylan, I.C., Matic, S., Myrta, A., Sipahioglu, H.M., Erkan, S. 2007. Occurrence and distribution of stone fruit viruses and viroids in commercial plantings of Prunus species in western Anatolia, Turkey, Journal of Plant Pathology, 89, 265–268.
  • Gürcan, K., Öcal, N., Yılmaz, K.U., Ullah, S., Erdoğan, A., Zengin, Y. 2015. Evaluation of Turkish apricot germplasm using SSR markers: Genetic diversity assessment and search for Plum pox virus resistance alleles. Scientia Horticulturae, 193: 155–164.
  • Ilardi, V., Tavazza, M. 2015. Biotechnological strategies and tools for Plum pox virus resistance: trans-, intra-, cis-genesis, and beyond. Frontiers in Plant Science, 6: 379.
  • İlbağı, H., Cıtır, A., Bostan, H. 2008. Prunus spinosa L. A natural wild host of some important fruit viruses in Tekirdağ, Turkey, Proceedings of the Twentieth International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops, Fruit Tree Diseases, Antalya, Turkey, Acta Horticulturae, 781, 33-36.
  • James, D., Varga, A. 2005. Nucleotide sequence analysis of Plum pox virüs isolate W3174: Evidence of a new strain, Virus Research, 110, 143–150.
  • Kalashayan, A., Bilkey, N. D., Verderevskaya, T.D., Rubina, E. V. 1994. Plum Pox Potyvirus on sour Cherry in Moldova. EPPO Bulletin 24: 645-649.
  • Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2): 111–120.
  • Kurçman S. 1973. Nachweis des sharka-virus an aprikosen und pflaumenbaumen aumenbaumen in Ankara. Journal of Turkish Phytopathology 2, 124–129.
  • Koç, G., Baloğlu, S. 2006. First report of sharka in the Çukurova region of Turkey, Journal of Plant Pathology, 88(3 suppl.), 68.
  • Lopez-Moya J. J., Fernandez-Fernandez, M. R., Cambra, M., Garcia, J. A. 2000. Biotechnological Aspects of Plum pox virus. J. Biotech. 76: 121-136.
  • Myrta, A., Varga, A., James, D. 2006. The complete genome sequence of an El Amar isolate of plum pox virus (PPV) and its phylogenetic relationship to other PPV strains, Archives of Virology, 151, 1189–1198.
  • Nemchinov, L., Hadidi, A., Maiss, E., Cambra, M., Candresse, T., Damsteegt, V. 1996. Sour cherry strain of plum pox potyvirus (PPV): molecular and serological evidence for a new subgroup of PPV strains, Phytopathology, 86, 1215–1221.
  • Ogawa M. J., Zehr, I. E., Bırd, W. G., Ritchie, F. D., Uriu, K., Uyemoto, K.J. 1995. Compendium of stone fruit diseases, Page: 69, The American Phytopathological Society Press.
  • Olmos, A., Cambra, M., Dasi, M.A., Candresse, T., Esteban, O., Gorris, M.T. & Asensio, M. 1997. Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA. Journal of Virological Methods, 68: 127–137.
  • Palkovics, L., Burgyán, J., Balázs, E. 1993. Comparative sequence analysis of four complete primary structures of plum pox virus strains, Virus Genes, 7, 339–347.
  • Scorza, R., Callahan, A., Dardick, C., Ravelonandro, M., Polak, J., Malinowski, T., Zagrai, I., Cambra, M., Kamenova, I. 2013. Genetic engineering of Plum pox virus resistance: ‘HoneySweet’ plum—from concept to product. Plant Cell Tiss. Organ. Cult., 115, 1–12.
  • Saitou, N., Nei, M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4:406-425.
  • Sertkaya, G., Ulubaş, Ç., Çağlayan, K.. 2003. Detection and characterization of Plum pox potyvirus (PPV) by DAS-ELISA and RTPCR/ RFLP analysis in Turkey, Turkish Journal of Agriculture and Forestry TÜBİTAK. Vol. 27, S.213-220.
  • Şahtiyancı S. 1969. Virus de la sharka chez le prunier. Bulletin Phytosanitaire FAO 17, 69.
  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. 2013. MEGA 6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30: 2725-2729.
  • Thompson J.D, Higgins, D.G., Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 4673-80.
  • Ulubaş Serçe, Ç., Candresse, T., Svanella-Dumas, L., Krizbai, L., Gazel, M., Çağlayan, K.. 2009. Further characterization of a new recombinant group of Plum pox virus isolates, PPV-T, found in the Ankara province of Turkey. Virus Research, 142: 121–126.
  • Ulubaş Serçe, Ç., Gazel, M., Çağlayan, K. 2011. Plum pox virus streynlerinin Türkiye’ deki Dağılımı (Distribution of Plum pox virus strains in Turkey), Türkiye IV. Bitki Koruma Kongresi Bildirileri, Kahramanmaraş s. 72.
  • Wetzel, T., Candresse, T., Ravelonandro, M., Dunez, J. 1991. A polymerase chain reaction assay adapted to plum pox potyvirus detection. Journal of Virological Methods, 33: 355–365.
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There are 52 citations in total.

Details

Primary Language Turkish
Journal Section Plant Protection
Authors

İlyas Deligöz

Kemal Değirmenci

Miray Sökmen

Publication Date December 8, 2015
Published in Issue Year 2015 Volume: 30 Issue: 3

Cite

APA Deligöz, İ., Değirmenci, K., & Sökmen, M. (2015). Samsun ilinde sert çekirdekli meyve türlerinde Şarka hastalığı etmeninin (Plum pox virus) belirlenmesi. Anadolu Tarım Bilimleri Dergisi, 30(3), 227-235. https://doi.org/10.7161/anajas.2015.30.3.227-235
AMA Deligöz İ, Değirmenci K, Sökmen M. Samsun ilinde sert çekirdekli meyve türlerinde Şarka hastalığı etmeninin (Plum pox virus) belirlenmesi. ANAJAS. October 2015;30(3):227-235. doi:10.7161/anajas.2015.30.3.227-235
Chicago Deligöz, İlyas, Kemal Değirmenci, and Miray Sökmen. “Samsun Ilinde Sert çekirdekli Meyve türlerinde Şarka hastalığı Etmeninin (Plum Pox Virus) Belirlenmesi”. Anadolu Tarım Bilimleri Dergisi 30, no. 3 (October 2015): 227-35. https://doi.org/10.7161/anajas.2015.30.3.227-235.
EndNote Deligöz İ, Değirmenci K, Sökmen M (October 1, 2015) Samsun ilinde sert çekirdekli meyve türlerinde Şarka hastalığı etmeninin (Plum pox virus) belirlenmesi. Anadolu Tarım Bilimleri Dergisi 30 3 227–235.
IEEE İ. Deligöz, K. Değirmenci, and M. Sökmen, “Samsun ilinde sert çekirdekli meyve türlerinde Şarka hastalığı etmeninin (Plum pox virus) belirlenmesi”, ANAJAS, vol. 30, no. 3, pp. 227–235, 2015, doi: 10.7161/anajas.2015.30.3.227-235.
ISNAD Deligöz, İlyas et al. “Samsun Ilinde Sert çekirdekli Meyve türlerinde Şarka hastalığı Etmeninin (Plum Pox Virus) Belirlenmesi”. Anadolu Tarım Bilimleri Dergisi 30/3 (October 2015), 227-235. https://doi.org/10.7161/anajas.2015.30.3.227-235.
JAMA Deligöz İ, Değirmenci K, Sökmen M. Samsun ilinde sert çekirdekli meyve türlerinde Şarka hastalığı etmeninin (Plum pox virus) belirlenmesi. ANAJAS. 2015;30:227–235.
MLA Deligöz, İlyas et al. “Samsun Ilinde Sert çekirdekli Meyve türlerinde Şarka hastalığı Etmeninin (Plum Pox Virus) Belirlenmesi”. Anadolu Tarım Bilimleri Dergisi, vol. 30, no. 3, 2015, pp. 227-35, doi:10.7161/anajas.2015.30.3.227-235.
Vancouver Deligöz İ, Değirmenci K, Sökmen M. Samsun ilinde sert çekirdekli meyve türlerinde Şarka hastalığı etmeninin (Plum pox virus) belirlenmesi. ANAJAS. 2015;30(3):227-35.

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