PCR‐based detection of resistance to ALS‐inhibiting herbicides of Bifora radians Bieb. (Bifra) in Wheat Fields

Year 2017, Volume: 32 Issue: 2, 153 - 163, 20.06.2017

Emine Kaya Altop Hüsrev Mennan Doğan Işık

https://doi.org/10.7161/omuanajas.320414

Cited By: 2

Abstract

Weeds are one of the most important
yield limiting factors in wheat which makes an effective weed control an
integral part of production. Due
to intensive herbicide use in wheat growing areas in Turkey is resistance
problems in some important weed species. There are some observations concerning
the efficacy failures of some ALS inhibitor herbicides on some important weed
species, such as Bifora radians.  With
this aim weed seeds will be collected from different wheat growing provinces in
Central Anatolia and Black Sea Region of Turkey, in total from 1371 different
wheat fields. After initial screening for resistance in pot experiments, biotypes
that are supposed to be resistant will be submitted to bioassays to determine
the resistance grades. Some accession
exhibited the highest survival to treatment with field rates of these
herbicides. The Weibull dose-response curve, an asymmetric sigmoid curve,
was fitted to data to obtain ED₅₀ in R program. Twenty-two of B. radians accessions tested did not controlled
by ALS inhibitor and they were accepted as a resistant population.  Based on the results of bioassays, resistant
biotypes tested by means of molecular methods. The gene region of resistant
biotypes was determined through sequence analyses and mutation wasn't found.

Keywords

Bifora radians, ALS inhibitor herbicides, Resistance, Bioassay, Molecular

Buğday ekim alanlarında sorun olan Bifora radians Bieb. (Kokarot)’ın ALS inhibitörü herbisitlere dayanıklılığının PCR temelli olarak belirlenmesi

Abstract

Yabancı otlar buğdayda verimi etkileyen en
önemli unsur olup yabancı ot mücadelesi buğday üretiminde önemli bir yer
tutmaktadır. Buğday ekim
alanlarında yoğun olarak herbisit kullanımına bağlı olarak bazı önemli yabancı
otlar kullanılan herbisitlere dayanıklılık geliştirmektedir. Bu amaçla geniş yapraklı tek yıllık rekabetçi bir bitki
olan ve birçok kültür bitkisinde olduğu gibi buğday ekim alanlarında da
sorun olan Bifora radians Bieb. yabancı otuna ruhsatlı ve mücadelede geniş kullanım alanı bulan ALS
(Acetolactatae synthase) inhibitörü herbisitlerle çalışılmıştır. Çalışmada
Türkiye’de buğday yetiştirilen ve bu yabancı otun sorun olduğu İç Anadolu ve
Orta Karadeniz Bölgesinin önemli illerinden toplam 1371 tarladan tohumlar
toplanmıştır. Serada saksı koşullarında bioassay yöntemleriyle dayanıklı
biyotipler ile bunların dayanıklılık dereceleri belirlenmiştir. Bazı populasyonların herbisit uygulamasından
etkilenmedikleri ve hayta kaldığı saptanmıştır. Bu populasyonlar doz-etki
çalışmaların alınmış ve ED₅₀ değerlerinin elde edilmesi için
Weibull modeli ile R paket programında analiz edilmiştir. Bifora radians’in 22
populasyonunun ALS inhibitorü herbisitlerle kontrol edilemediği ve dayanıklı
olduğu saptanmıştır. Ayrıca dayanıklılık gösteren biyotiplerde dayanıklılık,
moleküler yöntemler kullanılarak ve dayanıklılığa neden olan gen bölgesinin
sekans analizi yapılmak suretiyle tespit edilmiştir. Sekans sonucu çalışılan
türlerde mutasyon varlığına rastlanılmamıştır. 

Keywords

Bifora radians, ALS inhibitörü herbisitler, Dayanıklılık, Bioassay, Moleküler

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