Metarhizium anisopliae ve Beauveria bassiana izolat ve preparatlarının Curculio nucum’a karşı etkinlikleri

Year 2018, Volume: 33 Issue: 2, 116 - 123, 22.06.2018

Celal Tuncer , Rahman Kushiyev Jianfeng Liu İzzet Akça

https://doi.org/10.7161/omuanajas.387380

Cited By: 4

Abstract

BBu çalışmada, Metarhizium anisopliae (Metch) Sorok ve Beauveria bassiana (Bals.) Vuill. izolatları ve ticari
preparatlarının (Bio-Magic ve Bio-Power) laboratuvar koşullarında Curculio nucum L.’a karşı etkinliği
değerlendirilmiştir. Bu izolatlar ve ticari preparatların 1×108 spor mL-1
konsantrasyonları Potter ilaçlama kulesi kullanılarak petri içerisinde bulunan C. nucum erginleri üzerine
uygulanmıştır. Kontrol petri kaplarına ise % 0.02 Tween 20 içeren steril saf su
püskürtülmüştür. Petri kapları 25±1°C ve % 70±5 nemli ortamda 10 gün boyunca inkübe
edilmiştir. Ölü bireyler uygulamadan sonra 10 gün boyunca günlük olarak
sayılmıştır. Her bir gözlem günü için 4 tekerrür ve her petride 5 böcek olmak
üzere farklı böcek grupları kullanılmış (n=20 ergin böcek/gün/izolat veya
preparat) ve böylece zaman içindeki gözlemlerin ölüm üzerindeki bağımsızlığı
temin edilmiştir. Entomopatojenik funguslar için LT50 ve LT90 değerleri Probit
analizi ile hesaplanmıştır. Sonuç olarak, M.
anisopliae TR-106 izolatının LT50 ve LT90 değerleri sırasıyla 5.32 ve 7.85
gün iken, bu değerler Bio-Magic preparatı için sırasıyla 5.46 ve 10.16 gün
olarak belirlenmiştir. B. bassiana
TR-217 izolatı için aynı değerler sırasıyla 4.64 ve 8.53 gün olarak
bulunmuştur. Son olarak, Bio-Power preparatının LT50 ve LT90 değerleri
sırasıyla 6.16 ve 9.86 gün olarak tespit edilmiştir. Ayrıca, bu izolatlar ve
preparatlar 10 gün sonra C. nucum erginlerinde % 100 ölüme neden olmuştur. Bu
sonuçlar, M. anisopliae ve B. bassiana'nın fındık kurduna karşı
mücadelede umut verici biyolojik mücadele etmenleri olabileceğini
göstermektedir.

Keywords

Beauveria bassiana, Biyolojik mücadele, Curculio nucum, Metarhizium anisopliae, LT50, LT90

Efficacies of Metarhizium anisopliae and Beauvaria bassiana isolates and products against Curculio nucum

Abstract

In this study, the efficacies of Metarhizium anisopliae (Metch) Sorok and
Beauveria bassiana (Bals.) Vuill.
isolates and commercial products (Bio-Magic and Bio-Power) were tested against Curculio nucum L. under laboratory
conditions. Conidial suspensions of these isolates and products at
concentration of 1×108 conidia mL-1 were applied on the adults of C. nucum in petri dishes, using a Potter
spray tower. Control insects were sprayed only with sterile-distilled-water
containing 0.02 % Tween 20. All treated insects were incubated at 25±1°C and
70±5 % RH photoperiod in an incubator for 10 days.  Dead individuals were counted daily following
treatment for 10 days. Different groups of insects were used for each
observation days, with 4 replications and 5 insects per petri dish (n=20
insect/day/isolate or preparation), and thus independence of observations on
the mortality over time was ensured. Lethal time values (LT50 and LT90) for
entomopathogenic fungi were calculated using Probit analysis. As a result, LT50
and LT90 values of M. anisopliae
TR-106 isolate were 5.32 and 7.85 day, respectively while these values for
Bio-Magic product were determined as 5.46 and 10.16 days, respectively.  LT50 and LT90 for B. bassiana TR-217 isolate were found to be 4.64 and 8.53 day,
respectively. Finally, LT50 and LT90 values of Bio-Power product were
determined as 6.16 and 9.86 day, respectively. In addition, these isolates and
products have caused 100 % mortality on C. nucum adults within 10 days. The
results indicated that M. anisopliae
and B. bassiana could be promising
biological agents to control hazelnut weevil.

Keywords

Beauveria bassiana, Biological control, Curculio nucum, Metarhizium anisopliae, LT50, LT90

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