Determining WRKY transcription factors related to salinity stress response in wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.)

Yıl 2020, Cilt: 35 Sayı: 1, 1 - 7, 14.02.2020

Feyza Tufan

https://doi.org/10.7161/omuanajas.527704

Cited By: 3

Öz

The salinity is one of most common stress
factors with devastating effects for wheat (Triticum aestivum L.) and barley (Hordeum vulgare L). The
aim of this study was to determine the salt stress response associated with WRKY
transcription factors by gene expression analysis. Seeds of T. aestivum L. cvs.
Esperia and Bezozya, and H. vulgare L. cvs. Lord and Ramata were subjected to salt stress
for 10 days with two concentrations of NaCl (1.0 % and 2.0 %). No germination
was observed in 2.0 % NaCl treated seeds. Nearly all the WRKY genes studied were
upregulated and downregulated in response to 1.0 % NaCl stress in relatively
resistant and sensitive cultivars in comparison to control sets, respectively.
Among the screened genes, the expression of TaWRKY7, 40, 41, 53,
68, 72 and 79 genes were increased in relatively resistant
Esperia cultivar in wheat. Similarly, 7 of WRKY genes (HvWRKY6, 9, 24, 25,
33, 34, 42) were upregulated in relatively resistant Lord
cultivar in barley. In terms of WRKY gene expression profile, since TaWRKY7 and TaWRK72 in wheat and HvWRKY33 in
barley increased significantly, these genes can be used as marker genes for
further investigation of abiotic stress response. This study is a preliminary
study in terms of providing an association between WRKY genes and salinity stress response
of wheat and barley breeding cultivars in Turkey.

Anahtar Kelimeler

Gene expression, WRKY, Salt stress, Hordeum vulgare L., Triticum aestivum L.

Buğday (Triticum aestivum L.) ve arpada (Hordeum vulgare L.) tuzluluk stres yanıtı ile ilişkili WRKY transkripsiyon faktörlerinin belirlenmesi

Öz

Tuzluluk, buğday (Triticum
aestivum L.) ve arpa (Hordeum
vulgare L.) için yıkıcı
etkileri olan en yaygın stres faktörlerinden biridir. Bu çalışmada, WRKY transkripsiyon faktörleriyle ilişkili tuz
stresi yanıtının gen ekspresyon analizi ile belirlenmesi amaçlandı. T.
aestivum L. cvs. Esperia ve
Bezozya ile H. vulgare L.
cvs. Lord ve Ramata tohumlarına iki NaCl konsantrasyonu (% 1.0 ve % 2.0) ile 10
gün boyunca tuz stresi uygulandı. % 2.0 NaCl uygulanan tohumlarda çimlenme
gözlenmedi. Çalışılan hemen tüm WRKY genleri, kontrol gruplarına kıyasla % 1.0 NaCl stresine yanıt olarak
nispeten dirençli ve hassas kültivarlarda sırasıyla yukarı ve aşağı yönde
düzenlendi. İncelenen genler arasında, buğdayda nispeten dirençli Esperia
kültivarında TaWRKY7, 40, 41, 53, 68, 72 ve 79
genlerinin ekspresyonu indüklendi. Benzer şekilde, arpada nispeten dirençli
Lord’da 7 WRKY geni (HvWRKY6, 9, 24, 25, 33, 34,
42) indüklendi. WRKY gen ekspresyon profili açısından, buğdayda TaWRKY7 ve TaWRK72 ile arpada HvWRKY33 genlerinde anlamlı derecede artış saptanması nedeniyle bu genler abiyotik
stres yanıtının daha fazla araştırılması için marker genler olarak
kullanılabilir. Bu çalışma, Türkiye’de ekimi yapılan buğday ve arpa
çeşitlerinde WRKY genleri ve tuz stresi yanıtı arasında bir ilişki sağlaması açısından öncül
bir çalışma niteliğindedir.          

Anahtar Kelimeler

Gen ekspresyonu, WRKY, Tuz stresi, Hordeum vulgare L., Triticum aestivum L.

Kaynakça

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