Bazı rezene popülasyonlarının (Foeniculum vulgare Mill.) ISSR markörlerine dayalı moleküler varyasyonu

Year 2020, Volume: 35 Issue: 1, 140 - 146, 14.02.2020

Refika Giachino Betül Avcı

https://doi.org/10.7161/omuanajas.678277

Cited By: 1

Abstract

Bu araştırma, farklı rezene popülasyonları arasındaki moleküler varyasyonunu belirlemek amacıyla yürütülmüştür. Araştırmada materyal olarak farklı kaynaklardan sağlanan ve ikisi doğal floradan toplanmış olan 6 adet rezene popülasyonu kullanılmıştır. Popülasyonların moleküler karakterizasyonu 6 adet ISSR primeri ile belirlenmiştir. Toplam 57 bant elde edilmiş, bunlardan 48 adedi polimorfik olarak değerlendirilmiştir. Ortalama polimorfizm oranı %85 olarak bulunmuştur. PIC değerleri 0.33 (ISSR-16) ile 0.43 (ISSR-11) arasında değişim göstermiş, ortalama ise 0.37 olarak hesaplanmıştır. Primerlerin ortalama RP değeri 4.17 olarak hesaplanmış, en yüksek değer ISSR-4 (6.33) primerinden elde edilmiştir. Ortalama EMR değeri 6.78 bulunurken en yüksek değer ISSR-4 (10.29) primerinden elde edilmiştir. Ortalama MI değeri 2.47 olarak tahmin edildi, en yüksek değer yine ISSR-4 (3.67) primerinde gözlenmiştir. ISSR-4 primeri bu üç parametre ve en yüksek toplam ve polimorfik bant sayısı bakımından öne çıkan primer olmuştur. Rezene popülasyonları arasındaki benzerlik değerleri 0.30 ile 0.95 arasında bulunmuştur. Jaccard'ın benzerlik matrisleri ile küme sonucu arasındaki zihinsel test r = 0.99 (Kofenetik korelasyon katsayısı) olarak bulunmuştur ve ISSR tabanlı benzerlik matrisi ile küme arasında çok iyi bir korelasyon olduğunu göstermektedir. İncelenen ISSR primerleri bakımından ekimi ve satışı yapılan Burdur, Denizli, Isparta ve Izmir populasyonları birbirlerine genetik bakımdan oldukça benzer bulunurken doğal floradan toplanan Manisa Yuntdağı ve Ödemis popülasyonları ayrı gruplarda yer almışlardır.

Keywords

Rezene, ISSR, genetik farklılık, PIC, PCoA

Supporting Institution

Yok

ISSR-based molecular variation of some fennel (Foeniculum vulgare Mill.) populations

Abstract

This research was conducted to determine the molecular variation between different fennel populations. In this research, six fennel populations, of which two were collected from natural flora, were used as a material. The molecular characterization of the populations was performed using six ISSR (Inter simple sequence repeat) primers. A total of 57 bands were obtained, of which 48 were evaluated as polymorphic. The average polymorphism percentage was 85%. The PIC values changed from 0.33 (ISSR-16) to 0.43 (ISSR-11) with an average of 0.37. The average RP (Resolving Power) value of the primers was found as 4.17, and the highest value was obtained from the ISSR-4 (6.33) primer. The mean EMR value was 6.78, and the highest value was obtained from the ISSR-4 (10.29) primer. The mean MI (Marker Index) value was estimated to be 2.47, with the highest value similarly observed in the ISSR-4 (3.67) primer. ISSR-4 was the most prominent primer in terms of these three parameters and the maximum total and polymorphic bands. The similarity values of the fennel populations were found to be between 0.30 and 0.95. The Mental test conducted between Jaccard's similarity matrices and the cluster result revealed the r value as 0.99, showing a very good correlation between the ISSR-based similarity matrix and the cluster. The populations of Burdur, Denizli, Isparta and Izmir, which were cultivated and traded, were genetically similar in terms of the ISSR primers examined, whereas Manisa Yunt Mountain and Odemis populations collected from natural flora were in separate groups.

 

Keywords

Fennel, ISSR, genetic diversity, PIC, PCoA

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