Bazı rezene popülasyonlarının (Foeniculum vulgare Mill.) ISSR markörlerine dayalı moleküler varyasyonu

Yıl 2020, Cilt: 35 Sayı: 1, 140 - 146, 14.02.2020

Refika Giachino , Betül Avcı

https://doi.org/10.7161/omuanajas.678277

Cited By: 1

Öz

Bu araştırma, farklı rezene popülasyonları arasındaki moleküler varyasyonunu belirlemek amacıyla yürütülmüştür. Araştırmada materyal olarak farklı kaynaklardan sağlanan ve ikisi doğal floradan toplanmış olan 6 adet rezene popülasyonu kullanılmıştır. Popülasyonların moleküler karakterizasyonu 6 adet ISSR primeri ile belirlenmiştir. Toplam 57 bant elde edilmiş, bunlardan 48 adedi polimorfik olarak değerlendirilmiştir. Ortalama polimorfizm oranı %85 olarak bulunmuştur. PIC değerleri 0.33 (ISSR-16) ile 0.43 (ISSR-11) arasında değişim göstermiş, ortalama ise 0.37 olarak hesaplanmıştır. Primerlerin ortalama RP değeri 4.17 olarak hesaplanmış, en yüksek değer ISSR-4 (6.33) primerinden elde edilmiştir. Ortalama EMR değeri 6.78 bulunurken en yüksek değer ISSR-4 (10.29) primerinden elde edilmiştir. Ortalama MI değeri 2.47 olarak tahmin edildi, en yüksek değer yine ISSR-4 (3.67) primerinde gözlenmiştir. ISSR-4 primeri bu üç parametre ve en yüksek toplam ve polimorfik bant sayısı bakımından öne çıkan primer olmuştur. Rezene popülasyonları arasındaki benzerlik değerleri 0.30 ile 0.95 arasında bulunmuştur. Jaccard'ın benzerlik matrisleri ile küme sonucu arasındaki zihinsel test r = 0.99 (Kofenetik korelasyon katsayısı) olarak bulunmuştur ve ISSR tabanlı benzerlik matrisi ile küme arasında çok iyi bir korelasyon olduğunu göstermektedir. İncelenen ISSR primerleri bakımından ekimi ve satışı yapılan Burdur, Denizli, Isparta ve Izmir populasyonları birbirlerine genetik bakımdan oldukça benzer bulunurken doğal floradan toplanan Manisa Yuntdağı ve Ödemis popülasyonları ayrı gruplarda yer almışlardır.

Anahtar Kelimeler

Rezene, ISSR, genetik farklılık, PIC, PCoA

Destekleyen Kurum

Yok

ISSR-based molecular variation of some fennel (Foeniculum vulgare Mill.) populations

Öz

This research was conducted to determine the molecular variation between different fennel populations. In this research, six fennel populations, of which two were collected from natural flora, were used as a material. The molecular characterization of the populations was performed using six ISSR (Inter simple sequence repeat) primers. A total of 57 bands were obtained, of which 48 were evaluated as polymorphic. The average polymorphism percentage was 85%. The PIC values changed from 0.33 (ISSR-16) to 0.43 (ISSR-11) with an average of 0.37. The average RP (Resolving Power) value of the primers was found as 4.17, and the highest value was obtained from the ISSR-4 (6.33) primer. The mean EMR value was 6.78, and the highest value was obtained from the ISSR-4 (10.29) primer. The mean MI (Marker Index) value was estimated to be 2.47, with the highest value similarly observed in the ISSR-4 (3.67) primer. ISSR-4 was the most prominent primer in terms of these three parameters and the maximum total and polymorphic bands. The similarity values of the fennel populations were found to be between 0.30 and 0.95. The Mental test conducted between Jaccard's similarity matrices and the cluster result revealed the r value as 0.99, showing a very good correlation between the ISSR-based similarity matrix and the cluster. The populations of Burdur, Denizli, Isparta and Izmir, which were cultivated and traded, were genetically similar in terms of the ISSR primers examined, whereas Manisa Yunt Mountain and Odemis populations collected from natural flora were in separate groups.

 

Anahtar Kelimeler

Fennel, ISSR, genetic diversity, PIC, PCoA

Kaynakça

• Akcali Giachino, R.R., 2019. Investigation of the genetic variation of anise (Pimpinella anisum L.) using RAPD and ISSR markers, Genetic Resources and Crop Evolution, https://doi.org/10.1007/s10722-019-00861-y Akgul, A., 1993. Spice Science and Technology. Association of Food Technology Publ. 15, Ankara. Anderson JA, Churchill GA, Autrique JE, Tanksley SD, ME, Sorrells, 1993. Optimizing parental selection for genetic linkage maps. Genome 36:181–186. Azizi, N., Sheidai, M., Mozaffarian, V., Arman, M. & Noormohammadi, Z., 2019. Assessment of relationships among and within Helichrysum Mill. (Asteraceae) species by using ISSR markers and morphological traits, Hacquetia, 18/1, 105–118, DOI: 10.2478/hacq-2018-0014. Bahmani, K., Darbandi, A.I., Jafari, A.A., Noori, S.A.S.and Farajpour, M., 2012. Assessment of Genetic Diversity in Iranian Fennels Using ISSR Markers, Journal of Agricultural Science; Vol. 4, No. 9; 79-84. Bahmani, K., Darbandi, A.I., Noori, S.A.S. and Jafari, A.A., 2013. Assessment of the Genetic Diversity in Iranian Fennels by RAPD Markers, Journal of Herbs,Spices & Medicinal Plants, 19:3, 275-285. Baytop, T., 1999. Phytotherapy in Turkey. From past to present. 2. ed. Nobel Medical Publ İstanbul. Ceylan, A., 1997. Medicinal Plants-II (Essential Oil Plants). Agriculture Faculty of Ege University Pub 481. İzmir. Choudhary S, Sharma R, Meena RS, Verma AK., 2018. Molecular diversity analysis in fennel (Foeniculum vulgare Mill.) genotypes and its implications for conservation and crop breeding. International Journal of Current Microbiology and Applied Science. ; 7(03):794-809. El-Awadi, M. E., and A. H. Esmat. 2010. Physiological responses of fennel (Foeniculum vulgare Mill) plants to some growth substances. J. Am. Sci. 6(7):985–991. Farajpour, M., Ebrahimi, M., Amiri, R., Golzari, R., & Sanjari, S. 2012. Assessment of genetic diversity in Achillea millefolium accessions from Iran using ISSR marker. Biochem. Syst. Ecol, 43, 73-79. Gehan,G.M. and Alhamd, MFA., 2015. Induction of Salt Tolerant Mutants of Foeniculum vulgare by Dimethyl Sulphate and Their Identification Using Protein Pattern and ISSR Markers, Alex. J. Agric. Res. Vol. 60, No.2, pp. 95‐109. Godwin, I. D., Aitken, E. A. B., & Smith, L. W., 1997. Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis, 18, 1524-1528. Grover, S. and Malik, CP., 2017 Genetic Diversity and Identification of Variety-specific RAPD and ISSR Markers in Foeniculum vulgare ,- LS–An International Journal of Life Sciences, Indian journals.com, Vol: 6, Number 1, pp. 31-38. Jaccard, P 1908. Novelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270 Jadidi, O and Kalantar, M., 2016. Investigating Genetic Diversity of Foeniculum Vulgare Mill using Molecular Markers, International Journal of Advanced Biotechnology and Research (IJBR), Vol-7, Special Issue3,pp109-111, http://www.bipublication.com Kameli, M , Hesamzadeh Hejazi, S. M. and Ebadi, M., 2013. Assessment of genetic diversity on populations of three satureja species in Iran using ISSR markers, Annals of Biological Research, 4 (3):64-72. Lucinewton, S., N. Raul, J. Carvalho, B. Mirian, C. Lin, and A. Angela. 2005. Supercritical fluid extraction from fennel (Foeniculum vulgare) global yield, composition and kinetic data. J. of Supercritical Fluids 35:212–219. Maghsoudi Kelardashti H, Rahimmalek M, Talebi M. 2015. Genetic diversity in Iranian fennel (Foeniculum vulgare Mill.) populations based on sequence related amplified polymorphism (SRAP) markers. J Agric Sci Tech. 17:1789–1803. Mantel N, 1967. The detection of disease clustering and a generalized regression approach. Cancer Res 27:175–178 Milbourne D, Meyer R, Bradshaw JE, Baird E, Bonar N, Provan J, Powell W, Waugh R, 1997. Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated Potato. Mol Breed 3(2):127–136 Poudineh, Z., Fakheri, BA., Sirosmehr, AR., Shojaei, S., 2018. Genetic and morphological diversity of fennel by using ISSR marker and biplot analysis, Ind J Plant Physiol. 23(3):564–572, https://doi.org/10.1007/s40502-018-0390-8 Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A, 1996. The unity of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2:25–238 Prevost A, Wilkinson MJ., 1999. A new system of comparing PCR primers applied to ISSR fingerprinting. Theor Appl Genet 98:107–112 Rahimmalek, M., Bahreininejad, B., Khorrami, M., Tabatabaei, B.E.S, 2009. Genetic Variability and Geographic Differentiation in Thymus daenensis subsp. daenensis, an Endangered Medicinal Plant, as Revealed by Inter Simple Sequence Repeat (ISSR) Markers, Biochem Genet. 47:831–842. Rohlf, FJ., 2000. NTSYS-pc: numerical taxonomy and multivariate analysis system. Version 2.20j, user’s guide, New York. Salami, M., Rahimmalek, M., Ehtemam M.H., 2017. Genetic variability of outcross and selfed fennel based on morphological and ISSR markers, J. Agr. Sci. Technol. Vol:19, 157-172 Shafie, M. B., Sayed, S. M., Hasan, Z., & Shah, R. M. 2009. Study of genetic variability of Wormwood capillary (Artemisia capillaris) using inter simple sequence repeat (ISSR) in Pahang region, Malaysia. Plant Omics Journal, 2, 127-13. Shojaiefar, S., Mirlohi, A., Sabzalian, M.R. and Yaghini, H. 2015. Seed yield and essentialoil content of fennel influenced by genetic variation and genotype × year interaction. IndustrialCrops and Products. 71: 97-105. Yadav, C. and Malik, CP., 2018. Genetic Variation among Fennel (Foeniculum vulgare Mill.) Varieties on the basis of Essential Oil Composition and Molecular Markers (ISSR, SCoT, CDDP and CBDP), Jour Pl Sci Res 34 (1) 45-50. Yang,Y.X., Wu, W., Zheng, Y.L., Chen, L., Liu, R.J., Huang, C.Y., 2007. Genetic diversity and relationships among safflower (Carthamus tinctorius L.) analyzed by inter-simple sequence repeats (ISSRs), Genet Resour Crop Evol (2007) 54:1043–1051. Zietkiewicz, E, Rafalski, A and Labuda, D 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.