Association of SOD2 Polymorphism (Rs4880) and Competitive Performance in Track and Field Athletes

Year 2023, Volume: 14 Issue: 1, 59 - 68, 27.04.2023

Hasan Huseyin Kazan , Celal Bulğay , Erdal Zorba , Işık Bayraktar , Mesut Cerit , Mehmet Ali Ergun

https://doi.org/10.17155/omuspd.1205371

Abstract

The rs4880 polymorphism in the SOD2 gene has frequently been associated with the athletic performance of the athletes in different populations. However, there were not any studies in the Turkish elite athletes. Thus, the aim of the study is to evaluate the impacts of SOD2 rs4880 polymorphism on competitive performance of Turkish elite athletes. A total of 60 elite athletes (31 sprint/power and 29 endurance) and 20 controls with the ages of 18-35 voluntarily participated in the study. World Athletics (WA) score table was used to determine the performance levels of the competitive performance/personal best (PB) of the athletes. Whole exome sequencing (WES) was performed by the genomic DNA isolated from peripheral blood of the participants. Sport type, sex and competitive performance were chosen as the parameters to compare within and between the groups by linear regression models. There were not any significances between the genotype and allele frequencies. Finally, there were not any associations between competitive performance and genotype frequencies within the sprint/power athletes while competitive performance was significantly (p=0.049) correlated with rs4880 polymorphism according to the over-dominant model within the endurance athletes. The rs4880 polymorphism in the SOD2 gene may be linked to the competitive performance of the endurance athletes. Still, further studies with more participants and analyzing multiple factors are needed.

Keywords

Athlete, Athletic Performance, Athletics, rs4880 Polymorphism, SOD2

Supporting Institution

Gazi University Rectorate

Project Number

TCD-2021-7116

Elit Atletlerde SOD2 Rs4880 Polimorfizminin Yarışma Performansı ile İlişkisi

Abstract

SOD2 rs4880 polimorfizmi, farklı popülasyonlarda, atletlerin atletik performanslarıyla sıkça ilişkilendirilen varyantlardandır. Ancak, Türk elit atletlerde, bu varyantın atletik performans üzerine etkisine yönelik herhangi bir çalışma yoktur. Bu nedenle, sunulan çalışmanın amacı, elit atletlerde, SOD2 rs4880 polimorfizminin yarışma performansı üzerindeki olası etkilerini açığa çıkartmayı amaçlamaktadır. Çalışmaya 18-35 yaşları arasında 31 sprint/güç ve 29 dayanıklılık olmak üzere toplam 60 elit atlet ve 20 sedanter birey dahil edilmiştir. Atletlerin yarışma performansları/bireysel en iyi dereceleri (PB), Dünya Atletizm (WA) birliğinin performans puanlama sistemine göre belirlenmiştir. Katılımcıların kanlarından izole edilen genomik DNA ile tüm ekzom dizileme (WES) gerçekleştirilmiştir. Spor branşı, cinsiyet ve yarışma performansı, doğrusal regresyon modeli ile grup içi ve gruplar arası kıyaslanan parametreler olarak seçilmiştir. Grup içinde ve gruplar arasında, alel ve genotip frekansları arasında bir farklılık görülmemiştir. Sprint/güç grubu atletlerde, yarışma performansı ile genotip frekansı arasında anlamlı bir ilişki görülmezken dayanıklılık grubu atletlerde, rs4880 polimorfizminin yarışma performansı ile istatistiksel olarak anlamlı bir şekilde (p=0.049) ilişkili olduğu görülmüştür. Bu çalışma ile SOD2 rs4880 polimorfizminin dayanıklılık branşı ile ilişkili olabileceği söylenebilir. Yine de çok katılımlı bir grupla diğer genetik varyasyonların dahiliyle ileri çalışmalara ihtiyaç duyulmaktadır.

Keywords

Sporcular, Atletik Performans, Atletizm, rs4880 Polimorfizm, SOD2

Project Number

TCD-2021-7116

References

• Ahmetov, I. I., & Fedotovskaya, O. N. (2015). Current Progress in Sports Genomics. In Advances in Clinical Chemistry (Vol. 70, pp. 247–314). Academic Press Inc. doi: 10.1016/bs.acc.2015.03.003
• Ahmetov, I. I., Naumov, V. A., Donnikov, A. E., Maciejewska-Karłowska, A., Kostryukova, E. S., Larin, A. K., … Cieszczyk, P. (2014). SOD2 gene polymorphism and muscle damage markers in elite athletes. Free Radical Research, 48(8), 948–955. doi: 10.3109/10715762.2014.928410
• Akimoto, A. K., Miranda-Vilela, A. L., Alves, P. C. Z., Pereira, L. C. D. S., Lordelo, G. S., Hiragi, C. D. O., … Klautau-Guimarães, M. D. N. (2010). Evaluation of gene polymorphisms in exercise-induced oxidative stress and damage. Free Radical Research, 44(3), 322–331. doi: 10.3109/10715760903494176
• Antrobus, M. R., Brazier, J., Callus, P. C., Herbert, A. J., Stebbings, G. K., Khanal, P., … Heffernan, S. M. (2022). Concussion-Associated Polygenic Profiles of Elite Male Rugby Athletes. Genes, 13, 820.
• Ben-Zaken B, Eliakim A, Nemet D, Kassem E, & Meckel Y. (2013). Increased prevalence of MnSOD genetic polymorphism in endurance and power athletes. Free Radical Research, 47(12), 1001–1008.
• Ben-Zaken, S., Eliakim, A., Nemet, D., Rabinovich, M., Kassem, E., and Meckel, Y. (2015). ACTN3 polymorphism: comparison between elite swimmers and runners. Sports Medicine-Open, 1(1), 1–8.
• Bresciani, G., González-Gallego, J., da Cruz, I. B., de Paz, J. A., & Cuevas, M. J. (2013). The Ala16Val MnSOD gene polymorphism modulates oxidative response to exercise. Clinical Biochemistry, 46(4–5), 335–340. doi: 10.1016/j.clinbiochem.2012.11.020
• Bulgay, C., & Ergun, M. A. (2022). Athletic Performance, Genetics and Epigenetics. Egzersiz Fizyolojisi ve Temel Kavramlar. Ankara: Efe Akademi Yayınları.
• Bulgay, C., Kasakolu, A., Kazan, H. H., Mijaica, R., Zorba, E., Akman, O., ... & Ergun, M. A. (2023). Exome-Wide Association Study of Competitive Performance in Elite Athletes. Genes, 14(3), 660.
• De Morais Mourão, N. D., Lopes, J. S. S., Neto, A. M. M., Perego, S. M., Franca, A. C. H., & Franca, E. L. (2022). Behavior profile of cytokines submitted to combat sports. A systematic review. Science & Sports.
• Dokumacı B, & Cakır-Atabey H. (2016). Delayed Onset Muscle Soreness and Mechanisms: Relation with Oxidative Stress. Turkiye Klinikleri Journal of Sports Sciences, 8(1), 22–43.
• Forte, P., Branquinho, L., & Ferraz, R. (2022). The relationships between physical activity, exercise, and sport on the immune system. International Journal of Environmental Research and Public Health, 19(11), 6777.
• Ewa J., Wilhelm G., Jerzy S., Igor C., & Jadwiga K. (2017). SOD2 gene polymorphism may modulate biochemical responses to a 12- week swimming training. Free Radiccal Biology and Medicine, 113, 948–955.
• Gineviciene, V., Utkus, A.; Pranckevicˇienė, E.; Semenova, E.A.; Hall, E.C.R.; Ahmetov, I.I. (2022). Perspectives in Sports Genomics. Biomedicines. 10(2), 298.
• Jówko, E., Gierczuk, D., Cieśliński, I., & Kotowska, J. (2017). SOD2 gene polymorphism and response of oxidative stress parameters in young wrestlers to a three-month training. Free Radical Research, 51(5), 506–516. doi:10.1080/10715762.2017.1327716
• Jówko, E., Gromisz, W., Sadowski, J., Cieśliński, I., & Kotowska, J. (2017). SOD2 gene polymorphism may modulate biochemical responses to a 12-week swimming training. Free Radical Biology and Medicine, 113, 571–579. doi: 10.1016/j.freeradbiomed.2017.11.002
• Manica-Cattani, M. F., Cadoná, F. C., Oliveira, R. de, Silva, T. da, Machado, A. K., Barbisan, F., … Cruz, I. B. M. da. (2012). Impact of obesity and Ala16Val MnSOD polymorphism interaction on lipid, inflammatory and oxidative blood biomarkers. Open Journal of Genetics, 02(04), 202–209. doi:10.4236/ojgen.2012.24026
• Montano, M. A. E., da Cruz, I. B. M., Duarte, M. M. M. F., da Costa Krewer, C., de Ugalde Marques da Rocha, M. I., Mânica-Cattani, M. F., … Lera, J. P. B. (2012). Inflammatory cytokines in vitro production are associated with Ala16Val superoxide dismutase gene polymorphism of peripheral blood mononuclear cells. Cytokine, 60(1), 30–33. doi: 10.1016/j.cyto.2012.05.022
• Shimoda-Matsubayashi, S., Matsumine, H., Kobayashi, T., Nakagawa-Hattori, Y., Shimizu, Y., & Mizuno, Y. (1996). Structural dimorphism in the mitochondrial targeting sequence in the human manganese superoxide dismutase gene. A predictive evidence for conformational change to influence mitochondrial transport and a study of allelic association in Parkinson’s disease. Biochemical and biophysical research communications, 226(2), 561–565. Doi: 10.1006/bbrc.1996.1394
• Sole, X., Guino, E., Valls, J., & Iniesta, R. (2006). SNPStats: a web tool for the analysis of association studies. Bioinformatics, 22(15), 1928–1929.
• Spiriev, B. (2014). IAAF Scoring Tables of Athletics. Available online: https://www.worldathletics.org/news/iaaf- news/scoring-tables-2017 (accessed on 15 November 2022).
• Switala, K., & Leonska-Duniec, A. (2021). Physical Activity and Gene Association with Human Obesity. Balt. J. Health Phys. Act, 13, 99–111.
• Ulucan, K. (2016). Literature Review of Turkish Sportsmen in Terms of ACTN3 R577X Polymorphism. Clinical and Experimental Health Sciences, 6(1), 44–47. Retrieved from doi:10.5152/clinexphealthsci.2016.059
• Van der Van der Auwera, G. A., Carneiro, M. O., Hartl, C., Poplin, R., Del Angel, G., Levy-Moonshine, A., Jordan, T., Shakir, K., Roazen, D., Thibault, J., Banks, E., Garimella, K. V., Altshuler, D., Gabriel, S., & DePristo, M. A. (2013). From FastQ data to high confidence variant calls: the Genome Analysis Toolkit best practices pipeline. Current protocols in bioinformatics, 43(1110). Doi: 10.1002/0471250953.bi1110s43
• Varillas-Delgado, D., Del Coso, J., Gutiérrez-Hellín, J., Aguilar-Navarro, M., Muñoz, A., Maestro, A., & Morencos, E. (2022). Genetics and sports performance: the present and future in the identification of talent for sports based on DNA testing. European journal of applied physiology, 122(8), 1811–1830.
• Wang, K., Li, M., & Kakonarson, H. (2010). ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Research, 38(16), e168.
• Yıldırım, D. S., Erdoğan, M., Dalip, M., Bulğay, C., & Cerit, M. (2022) Evaluation of the soldier’s physical fitness test results (strength endurance) ın relation to genotype: longitudinal study. Egyptian Journal of Medical Human Genetics, 23(1), 114.