Staphylococcus epidermidis ve Staphylococcus aureus antibiyotik direncine sebep olan biyofilm oluşumunun belirlenmesi için kullanılan metotların kıyaslanması

Year 2017, Volume: 9 Issue: 1, 28 - 33, 31.03.2017

Sahra Kırmusaoğlu

https://doi.org/10.21601/ortadogutipdergisi.299940

Cited By: 1

Abstract

Öz

Amaç: Bu çalışma, tedavileri imkansız
ve zor olan, konak ve yabancı cisim ilişkili infeksiyonlar gibi tekrarlayabilen
infeksiyonları olan Staphylococcus aureus and Staphylococcus epidermidis gibi Stafilokoklar  tarafından oluşturulan biyofilmin belirlenmesinde
kullanılan MtP, TM ve CRA tarama metotlarını kıyaslamak için
gerçekleştirildi. 

Yöntem ve Gereç: Bu
çalışmada, 121 izolat kullanıldı ve altın standart metot olarak MtP metot
kullanıldı. Sensitivite, spesifisite, pozitif tahmin değeri ve negatif tahmin
değeri parametreleri hesaplandı. 

Bulgular: TM ve CRA
metotlarının sensitivite, spesifisiteleri, sırasıyla %97, %100 ve %87, %94 dür.

Tartışma: Bu çalışma Tüp Metodun CRA metottan daha güvenilir
olduğunu gösterdi. TM, laboratuvarlarda biyofilm oluşturan bakteri
identifikasyonu için ana tarama testi olarak tavsiye edilebilir. Güvenilir
biyofilm belirleme metodunun kullanımı ile yanlış tanılar ve tekrarlayan
infeksiyonlar önlenebilir.

Keywords

Biyofilm oluşumu, Staphylococcus epidermidis, Staphylococcus aureus, Mikrotitre plak methodu, tüp metodu, kongo kırmızısı agar metodu

The comparison of methods used for the detection of biofilm formation that cause antibiotic resistance of Staphylococcus epidermidis and Staphylococcus aureus

Abstract

                                                                   Abstract

Aim: This study was performed to compare
MtP, TM and CRA screening methods used for the detection of biofilm formation
by Staphylococcus spp. such as Staphylococcus aureus and Staphylococcus
epidermidis of which treatment can be impossible and hard, and infections such
as host and indwelling device-associated infections can be recurrent.

Materials and Methods: In this study 121 isolates were used
and MtP method was used as gold standart method. Sensitivity, specificity,
positive predictive value and negative predictive value parameters were
calculated.

Results: The sensitivity, specificity of TM
and CRA methods were 97%, 100% and 87%, 94%, respectively.

Conclusion: This study revealed that TM is more
reliable method than CRA method. TM can be recognized as the main screening
method for the identification of biofilm producer bacteria in the laboratories.
By the usage of reliable biofilm detection method, wrong diagnosises and
recurrent infections can be prevented.

Keywords

Biofilm formation, Staphylococcus epidermidis, Staphylococcus aureus, Microtiter plate method, Tube method, Congo red agar method

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