Staphylococcus epidermidis ve Staphylococcus aureus antibiyotik direncine sebep olan biyofilm oluşumunun belirlenmesi için kullanılan metotların kıyaslanması

Yıl 2017, Cilt: 9 Sayı: 1, 28 - 33, 31.03.2017

Sahra Kırmusaoğlu

https://doi.org/10.21601/ortadogutipdergisi.299940

Cited By: 1

Öz

Öz

Amaç: Bu çalışma, tedavileri imkansız
ve zor olan, konak ve yabancı cisim ilişkili infeksiyonlar gibi tekrarlayabilen
infeksiyonları olan Staphylococcus aureus and Staphylococcus epidermidis gibi Stafilokoklar  tarafından oluşturulan biyofilmin belirlenmesinde
kullanılan MtP, TM ve CRA tarama metotlarını kıyaslamak için
gerçekleştirildi. 

Yöntem ve Gereç: Bu
çalışmada, 121 izolat kullanıldı ve altın standart metot olarak MtP metot
kullanıldı. Sensitivite, spesifisite, pozitif tahmin değeri ve negatif tahmin
değeri parametreleri hesaplandı. 

Bulgular: TM ve CRA
metotlarının sensitivite, spesifisiteleri, sırasıyla %97, %100 ve %87, %94 dür.

Tartışma: Bu çalışma Tüp Metodun CRA metottan daha güvenilir
olduğunu gösterdi. TM, laboratuvarlarda biyofilm oluşturan bakteri
identifikasyonu için ana tarama testi olarak tavsiye edilebilir. Güvenilir
biyofilm belirleme metodunun kullanımı ile yanlış tanılar ve tekrarlayan
infeksiyonlar önlenebilir.

Anahtar Kelimeler

Biyofilm oluşumu, Staphylococcus epidermidis, Staphylococcus aureus, Mikrotitre plak methodu, tüp metodu, kongo kırmızısı agar metodu

The comparison of methods used for the detection of biofilm formation that cause antibiotic resistance of Staphylococcus epidermidis and Staphylococcus aureus

Öz

                                                                   Abstract

Aim: This study was performed to compare
MtP, TM and CRA screening methods used for the detection of biofilm formation
by Staphylococcus spp. such as Staphylococcus aureus and Staphylococcus
epidermidis of which treatment can be impossible and hard, and infections such
as host and indwelling device-associated infections can be recurrent.

Materials and Methods: In this study 121 isolates were used
and MtP method was used as gold standart method. Sensitivity, specificity,
positive predictive value and negative predictive value parameters were
calculated.

Results: The sensitivity, specificity of TM
and CRA methods were 97%, 100% and 87%, 94%, respectively.

Conclusion: This study revealed that TM is more
reliable method than CRA method. TM can be recognized as the main screening
method for the identification of biofilm producer bacteria in the laboratories.
By the usage of reliable biofilm detection method, wrong diagnosises and
recurrent infections can be prevented.

Anahtar Kelimeler

Biofilm formation, Staphylococcus epidermidis, Staphylococcus aureus, Microtiter plate method, Tube method, Congo red agar method

Kaynakça

• Fluckiger U, Ulrich M, Steinhuber A, Döring G, Mack D, Landmann R, et al. Biofilm formation, icaADBC transcription and polysaccharide intercellular adhesin synthesis by staphylococci in a device-related infection model. Infect Immun 2005;73:1811–9.
• Gristina AG, Oga M, Webb LX, Hobgood CD. Adherent bacterial colonization in the pathogenesis of osteomyelitis. Science 1985;228:990–3.
• Trampuz A, Zimmerli W. Prostetic joint infections: update in diagnosis and treatment. Swiss MedWkly 2005;135:243–51.
• Archer NK, Mazaitis MJ, Costerton JW, Leid JG, Powers ME, Shirtliff ME. Staphylococcus aureus biofilms: properties, regulation, and roles in human disease. Virulence 2011;2:445–459.
• Kuroda M, Ito R, Tanaka Y, Yao M, Matoba K, Saito S, et al. Staphylococcus aureus surface protein SasG contributes to intercellular autoaggregation of Staphylococcus aureus. Biochem Biophys Res Commun 2008;377:1102-6.
• O’Neill E, Pozzi C, Houston P, Humphreys H, Robinson DA, et al. A novel Staphylococcus aureus biofilm phenotype mediated by the fibronectin-binding proteins, FnBPA and FnBPB. J Bacteriol 2008;190:3835-50.
• Heilmann C, Schweitzer O, Gerke C, Vanittanakom N, Mack D, Götz F. Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol 1996;20:1083-91.
• Taconelli E, Smith G, Hieke K, Lafuma A, Bastide P. Epidemiology, medical outcomes and costs of catheter-related bloodstream infection in intensive care units of four European countries: litterature- and registry-based estimates. J Hosp Infect 2009;72:97–103.
• Kolenbrander PE, Palmer Jr RJ. Human oral bacterial biofilms. In: Ghannoum MA, O’Toole GA, editors. Microbial biofilms. Washington, DC: ASM Press; 2004.
• Hall-Stoodley L, Hu FZ, Gieseke A, Nistico A, Nguyen L, Hayes J, et al. Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media. JAMA 2006;296:202–11.
• Bjarnsholt T, Jensen PØ, Fiandaca MJ, Pedersen J, Hansen CR, Andersen CB, et al. Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients. Pediatr Pulmonol 2009;44:547–58.
• Del Pozo JL, Patel R. Infection associated with prostetic joints. N Engl J Med 2009;361:787–94.
• Christensen GD, Simpson WA, Younger JA et al. Adherence of coagulase negative Staphylococci to plastic tissue cultures: a quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol 1995;22:996-1006.
• Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun 1982;37:318-26.
• Freeman J, Falkiner FR, Keane CT. New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol 1989;42:872-4.
• Donlan RM, Murga R, Bell M et al. Protocol for detection of biofilms on needleless connectors attached to central venous catheters. J Clin Microbiol 2001;39:750-3.
• Aparna MS, Yadav S. Biofilms: microbes and disease. Braz J Infect Dis 2008;12(6):526-30.
• Zufferey J, Rime B, Francioli P, Bille J. Simple method for rapid diagnosis of catheter associated infection by direct Acridine orange staining of catheter tips. J Clin Microbiol 1988;26:175-7.
• Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, et al. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. Journal of Clinical Microbiology 1985;22(6):996–1006.
• Stepanović S, Vuković D, Daki I, Savic B, Vlahovic-Svabic M. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods 2000;40:175-179.
• Stepanović S, Vuković D, Hola V, Di Bonaventura G, Djukić S, Ćirković I, Ruzicka F. Quantification of biofilm in microtiter plates: overview of testing conditions and partical recommendations for assessment of biofilm production by staphylococci. APMIS 2007;115:891–899.
• De Castro Melo P, Ferreira LM, Filho AN, Zafalon LF, Vicente HIG, de Souza V. Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis. Brazilian Journal of Microbiology 2003;44:(1),119-124.
• Jain A, Agarwal A. Biofilm production, a marker of pathogenic potential of colonizing and commensal staphylococci. J Microbiol Methods 2009;76:88-92.
• Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M. Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis 2011;15(4):305-311.
• Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods. Indian J Med Microbiol 2006;24(1):25-9.
• Ruzicka F, Hola V, Votava M et al. Biofilm detection and clinical significance of Staphylococcus epidermidis isolates. Folia Microbiol (Praha) 2004;49(5):596-600.
• Baqai R, Aziz M, Rasool G. Urinary tract infection in diabetic patients and biofilm formation of uropathogens. Infect Dis J Pakistan. 2008;17(1):7-9.
• Knobloch JK, Horsetkotte MA, Rohde H, Mack D. Evaluation of different detection methods of biolfilm formation in Staphylococcus aureus. Med Microbial Immunol 2002;191(2):101-6.
• Kumar Gupta M, Gahlot R, Nigam C, Kumar V. Biofilm: Detection Methods and Correlation with Antimicrobial Resistance in Staphylococcus. National Journal of Laboratory Medicine 2013;2(2):7-10.
• Dhanawade NB, Kalorey DR, Srinivasan R, Barbuddhe SB, Kurkure NV. Detection of intercellular adhesion genes and biofilm production in Staphylococcus aureus isolated from bovine subclinical mastitis. Vet Res Commun 2010;34:81-89.