Aziz Sancar

Öz

Bu derlemede halen North Carolina Üniversitesi Tıp Fakültesi, Chapel Hill'de akademik çalışmalarına devam etmekte olan Prof.Dr. Aziz Sancar'in otobiyografisi ve akademik çalışmaları yer almaktadır.

Anahtar Kelimeler

• Nobel
• Biyolojik saat
• DNA tamiri
• Kromofor

Aziz Sancar

Öz

In this review, autobiography and academic studies of Professor Aziz Sancar, currently working at the University of North Carolina School of Medicine, Chapel Hill, are summarized.

Anahtar Kelimeler

• Nobel
• Biological clock
• DNA repair
• Chromophor

Kaynakça

1. 1. Aziz Sancar – Biographical. NobelPrize.org. Nobel Prize Outreach AB 2023. https://www.nobelprize.org/prizes/chemistry/2015/ sancar/biographical).
2. 2. Bursalı O. Aziz Sancar ve Nobel’in öyküsü. Kırmızı Kedi Yayınevi. 2016.
3. 3. Pehlivanoglu B, Aysal A, Kececi SD ve ark. A Nobel-Winning scientist: Aziz Sancar and the impact of his work on the molecular pathology of neoplastic diseases. Turk Patoloji Derg. 2021;37(2):93-105.
4. 4. Sancar A, Rupert CS. Cloning of the phr gene and amplification of photolyase in Escherichia coli. Gene. 1978; 4:295-308.
5. 5. Sancar A, Hack AM, Rupp WD. Simple method for identification of plasmid-coded proteins. J. Bacteriology. 1979; 137:692-693.
6. 6. Sancar A, Clarke ND, Griswold J, Kennedy WJ, Rupp WD. Identification of the uvrB gene product. J Mol Biol. 1981a; 148: 63-76.
7. 7. Sancar A, Kacinski BM, Mott DL, Rupp WD (). Identification of the uvrC gene product. Proc Natl Acad Sci USA. 1981b; 78: 5450-5454.
8. 8. Sancar A, Wharton RP, Seltzer S, Kacinski BM, Clarke ND, Rupp WD. Identification of the uvrA gene product. J Mol Biol. 1981c; 148: 45-62.

9. 9. Sancar A, Sancar GB, Rupp WD, Little JW, Mount DW. LexA protein inhibits transcription of the E. coli uvrA gene in vitro. Nature. 1982a; 298: 96-98.
10. 10. Sancar GB, Sancar A, Little JW, Rupp WD (). The uvrB gene of Escherichia coli has both lexA-repressed and lexA-independent promoters. Cell. 1982b; 28: 523-530.
11. 11. Sancar A, and Rupp WD. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region. Cell. 1983; 33: 249-260.
12. 12. Sancar GB, Sancar A, and Rupp WD (1984). Sequences of the E. coli uvrC gene and protein. Nucleic Acids Res 12, 4593-4608.
13. 13. Beck DJ, Popoff S, Sancar A, Rupp W.D. (1985). Reactions of the UVRABC excision nuclease with DNA damaged by diamminedichloroplatinum(II). Nucleic Acids Res 13, 7395-12.
14. 14. Sancar A, Sancar GB. (1984). Escherichia coli DNA photolyase is a flavoprotein. J Mol Biol. 172: 223-7. 15. Sancar GB, Smith FW, Lorence MC, Rupert CS, Sancar A. Sequences of the Escherichia coli photolyase gene and protein. J Biol Chem. 1984; 259: 6033-6038.
15. 16. Markham BE, Harper JE, Mount DW et al. Analysis of mRNA synthesis following induction of the Escherichia coli SOS system. J Mol Biol. 1984;178: 237-248.
16. 17. Arikan E, Kulkarni MS, Thomas DC, Sancar A. Sequences of the E. coli uvrB gene and protein. Nucleic Acids Res. 1986; 14: 2637-2650.
17. 18. Liu Z, Tan C, Guo X, Kao YT, Li J, Wang L, Sancar A, Zhong D. Dynamics and mechanism of cyclobutane pyrimidine dimer repair by DNA photolyase. Proc Natl Acad Sci USA. 2011; 108:14831-14836.
18. 19. Husain I, Van Houten B, Thomas DC, Abdel-Monem M, Sancar A. Effect of DNA polymerase I and DNA helicase II on the turnover rate of UvrABC excision nuclease. Proc Natl Acad Sci USA. 1985; 82: 6774-6778.
19. 20. Doolittle RF, Johnson MS, Husain I, Van Houten B, Thomas DC, Sancar A. Domainal evolution of a prokaryotic DNA repair protein and its relationship to active-transport proteins. Nature. 1986; 323: 451-453.
20. 21. Van Houten B, Gamper H, Hearst JE, Sancar A. Construction of DNA substrates modified with psoralen at a unique site and study of the action mechanism of ABC excinuclease on these uniformly modified substrates. J Biol Chem. 1986a; 261:14135-14141.
21. 22. Van Houten B, Gamper H, Holbrook SR, Hearst, JE, Sancar A. Action mechanism of ABC excision nuclease on a DNA substrate containing a psoralen crosslink at a defined position. Proc Natl Acad Sci USA 1986b; 83: 8077-8081.
22. 23. Van Houten B, Gamper H, Sancar A, Hearst JE. DNase I footprint of ABC excinuclease. J Biol Chem. 1987; 262:13180-13187.
23. 24. Van Houten B, Gamper H, Hearst JE, Sancar A. Analysis of sequential steps of nucleotide excision repair in Escherichia coli using synthetic substrates containing single psoralen adducts. J Biol Chem 1988; 263:16553-16560.
24. 25. Orren DK, and Sancar A. The (A)BC excinuclease of Escherichia coli has only the UvrB and UvrC subunits in the incision complex. Proc Natl Acad Sci USA 1989; 86: 5237-5241.
25. 26. Lin JJ, Sancar A. A new mechanism for repairing oxidative damage to DNA: (A)BC excinuclease removes AP sites and thymine glycols from DNA. Biochemistry. 1990; 28: 7979-7984.
26. 27. Selby CP, Witkin EM, and Sancar A. Escherichia coli mfd mutant deficient in "mutation frequency decline" lacks strand-specific repair: in vitro complementation with purified coupling factor. Proc Natl Acad Sci USA 1991; 88: 11574-11578.
27. 28. Selby CP, Sancar A. Gene- and strand-specific repair in vitro: partial purification of a transcription-repair coupling factor. Proc Natl Acad Sci USA 1991; 88: 8232-8236.
28. 29. Selby CP, Sancar A. Molecular mechanism of transcription-repair coupling. Science 1993; 260: 53-58.
29. 30. Huang JC, Svoboda DL, Reardon JT, Sancar A. Human nucleotide excision nuclease removes thymine dimers from DNA by incising the 22nd phosphodiester bond 5' and the 6th phosphodiester bond 3' to the photodimer. Proc Natl Acad Sci USA. 1992; 89:3664-3668.
30. 31. Koshland DE. Molecule of the year: the DNA repair enzyme. Science. 1994; 266:1925.
31. 32. Petit C, Sancar A. Nucleotide excision repair: from E. coli to man. Biochimie 1999; 81:15-25.
32. 33. Hu J, Adar S, Selby CP, Lieb JD, Sancar A. Genome-wide analysis of human global and transcription-coupled excision repair of UV damage at single-nucleotide resolution. Genes Dev. 2015; 29:948-960.
33. 34. Sancar A. Cryptochrome: the second photoactive pigment in the eye and its role in circadian photoreception. Annu Rev Biochem. 2000; 69:31-67.
34. 35. Hsu DS, Zhao X, Zhao S et al. Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins. Biochemistry. 1996; 35:13871-13877.
35. 36. Miyamoto Y, Sancar A.Vitamin B2-based blue-light photoreceptors in the retinohypothalamic tract as the photoactive pigments for setting the circadian clock in mammals. Proc Natl Acad Sci USA. 1998; 95:6097-6102.
36. 37. Thresher RJ, Vitaterna MH, Miyamoto Y et al. Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses. Science (New York, N.Y.) 1998; 282:1490-1494.
37. 38. Gauger MA, Sancar A. Cryptochrome, circadian cycle, cell cycle checkpoints, and cancer. Cancer Research. 2005; 65: 6828-6834.
38. 39. Sancar A, Lindsey-Boltz LA, Kang TH et al. Circadian clock control of the cellular response to DNA damage. FEBS Lett. 2010; 584: 2618-2625.
39. 40. Choi JH, Gaddameedhi S, Kim SY et al. Highly specific and sensitive method for measuring nucleotide excision repair kinetics of ultraviolet photoproducts in human cells. Nucleic Acids Research. 2014;42: e29.