Investigation of Vaspin and Visfatin -4689G/T Gene Polymorphisms in Alopecia Areata Patients

Öz

Alopecia Areata (AA) is a chronic autoimmune condition that causes recurrent hair bereavement. Genetic and immunological factors act a part in the pathogenesis of AA. The aim of this study was to look into relationship between the vaspin and visfatin -4689G/T gene polymorphisms and AA sensibility in the Turkish population. This study included 80 AA patients and 80 healthy controls. Genomic DNA was extracted of blood samples Vaspin and visfatin -4689G/T gene polymorphisms were determined using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. The observed disparity in vaspin genotypes and allele distribution amid AA patients and healthy controls did not reach statistical significance (χ2 = 2.51, df = 1, p = 0.11 and χ2 = 1.75, df = 1, p = 0.18, respectively). Although visfatin GT genotype was higher in AA patients compared to control, it was not statistically significant. People with the visfatin GT genotype were more likely to be AA than people with the GG genotype [OR (95% CI) = 2.11 (1.04-4.27), p = 0.03]. This study shows that there is no affair amid vaspin and visfatin -4689G/T polymorphism and AA in the Turkish population. However, the TT genotype for the vaspin gene and the GT genotype for the visfatin -4689G/T gene are risk factors for people with AA disease.

Anahtar Kelimeler

• Alopecia areata
• adipokine
• visfatin
• single nucleotide polymorphism

Alopesi Areata Hastalarında Vaspin ve Visfatin -4689G/T Gen Polimorfizmlerinin Araştırılması

Öz

Alopecia Areata (AA), tekrarlayan saç kaybına neden olan kronik bir otoimmün durumdur. AA patogenezinde genetik ve immünolojik faktörler rol oynamaktadır. Bu çalışmanın amacı Türk toplumunda vaspin ve visfatin -4689G/T gen polimorfizmleri ile AA duyarlılığı arasındaki ilişkiyi araştırmaktır. Bu çalışmaya 80 AA hastası ve 80 sağlıklı kontrol dahil edildi. Kan örneklerinden genomik DNA elde edildi. Vaspin ve visfatin -4689G/T gen polimorfizmleri, polimeraz zincir reaksiyonu (PCR) ve restriksiyon fragman uzunluğu polimorfizmi (RFLP) yöntemleri kullanılarak belirlendi. AA hastaları ve sağlıklı kontroller arasında vaspin genotipleri ve alel dağılımında gözlenen farklılık istatistiksel anlamlılığa ulaşmadı (sırasıyla, χ2 = 2.51, df = 1, p = 0.11 and χ2 = 1.75, df = 1, p = 0.18). Visfatin GT genotipi AA hastalarında kontrole göre daha yüksek olmasına rağmen istatistiksel olarak anlamlı değildi. Visfatin GT genotipine sahip kişilerin AA olma olasılığı, GG genotipine sahip kişilere göre daha yüksekti [OR (95% CI) = 2.11 (1.04-4.27), p = 0.03]. Bu çalışma Türk toplumunda vaspin ve visfatin -4689G/T polimorfizmi ile AA arasında bir ilişki olmadığını göstermektedir. Bununla birlikte, vaspin geni için TT genotipi ve visfatin -4689G/T geni için GT genotipi AA hastalığı olan kişiler için risk faktörleridir.

Anahtar Kelimeler

• Alopesi areata
• adipokin
• visfatin
• tek nükleotid polimorfizmi

Kaynakça

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