Apoptotic Effects of Urtica dioica extract and Boric Acid in MCF-7 Breast Cancer Cell Line: A Study

Öz

This study investigates the effects of Urtica dioica (nettle), Boric acid, and their combinations on MCF-7 human breast cancer cells. The goal is to explore how these substances impact apoptosis (programmed cell death) and anti-apoptotic pathways in a breast cancer model while assessing the protective effects of Urtica dioica and Boric acid.The MTT assay was employed to measure cell viability, and IC50 doses were determined. Protein levels of BAX, p53, Cytochrome C, and Calpain were quantified using the ELISA method. Additionally, phytochemical analyses were conducted using HPLC.Results indicated a statistically significant increase in p53 levels between the control group and those treated with Urtica dioica and Boric acid (p<0.001). Cytochrome C levels also showed a significant increase in the Urtica dioica group, with Boric acid demonstrating a meaningful increase (p<0.001, p<0.01). HPLC analysis of Urtica dioica extract identified caffeic acid as one of the critical phytochemicals.The findings suggest that combining Urtica dioica and Boric acid has limited effects on apoptosis in breast cancer cells, although positive impacts on p53 and Cytochrome C were noted. These results provide a foundation for considering Urtica dioica and Boric acid in breast cancer treatment. However, further clinical and animal studies are needed to evaluate these substances' efficacy, safety, and appropriate dosages. Future research will deepen our understanding of Urtica dioica and Boric acid's roles in breast cancer therapy and help develop new treatment strategies.

Anahtar Kelimeler

• Breast cancer
• MCF-7
• Urtica dioica
• Boric acid
• Apoptosis

Urtica dioica ve Borik Asidin MCF-7 Meme Kanseri Hücre Hattındaki Apoptoz Etkileri: Bir Çalışma

Öz

Bu çalışmanın amacı, Urtica dioica (ısırgan), borik asit ve bunların kombinasyonlarının MCF-7 insan meme kanseri hücreleri üzerindeki etkilerini değerlendirmektir. İki maddenin apoptozu (programlı hücre ölümü) ve anti-apoptotik yolları nasıl etkilediğini araştırmak ve Urtica dioica ile borik asidin koruyucu etkilerini belirlemektir.Hücre canlılığını değerlendirmek için MTT testi kullanılmış ve hücreler için IC50 dozları belirlenmiştir. Ayrıca, hücrelerde BAX, p53, Sitokrom C ve Kalpain protein düzeyleri ELISA yöntemi ile ölçülmüştür. Fito-kimyasal analizler ise HPLC yöntemi ile gerçekleştirilmiştir.P53 konsantrasyon ölçümleri, kontrol grubu ile Urtica dioica ve borik asit ile tedavi edilen gruplar arasında istatistiksel olarak anlamlı bir artış göstermiştir (p<0.001). Sitokrom C konsantrasyon ölçümleri, kontrol grubu ile Urtica dioica arasında çok anlamlı bir artış, borik asit için ise anlamlı bir artış göstermiştir (p<0.001, p<0.01). Ayrıca, Urtica dioica ekstresi HPLC ile analiz edilerek, kafeik asit fito-kimyasallardan biri olarak belirlemektir. Bu araştırma, Urtica dioica ve borik asidin kombinasyonunun meme kanseri hücrelerinde apoptoz üzerindeki etkilerinin sınırlı olduğunu göstermektedir. Bu bileşenlerin p53 ve Sitokrom C üzerindeki olumlu etkileri gözlemlenmiştir. Bu bulgular, Urtica dioica ve borik asidin meme kanseri tedavisinde potansiyel kullanımına zemin hazırlamaktadır. Ancak, bu maddelerin etkinliğini, güvenliğini ve uygun dozlarını belirlemek için daha fazla klinik ve hayvan çalışmasına ihtiyaç vardır. Gelecek araştırmalar, Urtica dioica ve borik asidin meme kanseri tedavisindeki rollerini anlamamıza katkı sağlayacak ve yeni tedavi stratejilerinin geliştirilmesine yardımcı olacaktır.

Anahtar Kelimeler

• Meme kanseri
• MCF-7
• Isırgan Otu
• Borik asit
• Apoptoz

Kaynakça

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