Intracerebroventricularly Administered Apelin-13 Regulates Cardiovascular Parameters and SIRT1 Expression

Yıl 2026, Cilt: 48 Sayı: 2, 343 - 351, 11.02.2026

Serdar Şahintürk , Naciye İşbil , Betül Çam , Ayşen Çakır , Cansu Badem , Mertcan Tekneci , Kasım Özlük

https://doi.org/10.20515/otd.1818898

https://izlik.org/JA83CK36NY

Öz

In this work, we examined the effects of apelin-13 intracerebroventricular injection on heart rate, blood pressure, cardiac contractility, and cardiac sirtuin 1 (SIRT1) level. Wistar rats received intracerebroventricular injections of either 10 µl saline or Apelin-13 (5, 10, and 20 nmol). First, heart rate and mean arterial blood pressure (MABP) were recorded. The second step was determining the left ventricular ±dP/dt values. After the rats' hearts were removed, the Western Blot method was used to measure the amount of SIRT1 in the heart tissues. 10 nmol of apelin-13 raised MABP. On the other hand, 20 nmol of apelin-13 reduced MABP. At 5 and 10 nmol, apelin-13 reduced heart rate. On the other hand, apelin-13 raised heart rate at a dose of 20 nmol. Apelin-13 raised +dP/dt values at 10 and 20 nmol dosages. -dP/dt readings were raised by apelin-13 at a dose of 20 nmol. SIRT1 levels in the cardiac tissues were raised by apelin-13 at a dose of 5 nmol. These results confirm that apelin is involved in the central control of heart rate, blood pressure, and cardiac contractility. The current investigation shows that apelin administered centrally improves left ventricular contractile function. Additionally, this study suggests that SIRT1 might be involved in the physiological effects of apelin on the cardiovascular system.

Anahtar Kelimeler

apelin-13 , blood pressure , intracerebroventricular , positive inotropic , SIRT1

Etik Beyan

Ethical approval for this study was given by Bursa Uludağ University Animal Experiments Local Ethics Committee (number: 2015-07/06; date: 26.05.2015).

Destekleyen Kurum

Bursa Uludağ University Scientific Research Projects Unit

Proje Numarası

Proje numarası: OUAP(T)-2015/24.

Teşekkür

We would like to thank Bursa Uludağ University Scientific Research Projects Unit.

İntraserebroventriküler Uygulanan Apelin-13 Kardiyovasküler Parametreleri ve SIRT1 Ekspresyonunu Düzenler

https://izlik.org/JA83CK36NY

Öz

Bu çalışmada, intraserebroventriküler apelin-13 enjeksiyonunun kan basıncı, kalp hızı, kardiyak kontraktilite ve kardiyak sirtuin 1 (SIRT1) düzeyi üzerindeki etkilerini araştırdık. Apelin-13 (5, 10 ve 20 nmol) veya 10 µl salin, Wistar sıçanlarına intraserebroventriküler olarak enjekte edildi. İlk olarak, ortalama arteriyel kan basıncı (MABP) ve kalp hızı ölçüldü. İkinci olarak sol ventrikül ±dP/dt değerleri belirlendi. Son olarak sıçanların kalpleri çıkarıldı ve kalp dokularındaki SIRT1 seviyesi Western Blot yöntemi ile belirlendi. 10 nmol dozundaki apelin-13 MABP'yi artırdı. Bununla birlikte, 20 nmol dozundaki apelin-13 MABP'yi azalttı. 5 ve 10 nmol dozlarındaki apelin-13 kalp hızını azalttı. Bununla birlikte, 20 nmol dozundaki apelin-13 kalp hızını artırdı. 10 ve 20 nmol dozlarında apelin-13 +dP/dt değerlerini artırdı. 20 nmol dozunda apelin-13 –dP/dt değerlerini artırdı. 5 nmol dozundaki apelin-13, kalp dokularındaki SIRT1 düzeyini artırdı. Bu bulgular, apelinin kan basıncının, kalp hızının ve kardiyak kontraktilitenin merkezi düzenlemesine katıldığı gerçeğini desteklemektedir. Bu çalışma, apelinin santral uygulamasının sol ventrikül kontraktil performansını artırdığını göstermektedir. Ayrıca bu çalışma, SIRT1'in apelinin kardiyovasküler fizyolojik etkilerinde rol oynayabileceğine işaret etmektedir.

Anahtar Kelimeler

apelin-13 , intraserebroventriküler , kan basıncı , pozitif inotropik , SIRT1

Etik Beyan

Bu çalışma için etik onay, Bursa Uludağ Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu tarafından verilmiştir (numara: 2015-07/06; tarih: 26.05.2015).

Destekleyen Kurum

Bursa Uludağ Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

Proje numarası: OUAP(T)-2015/24.

Teşekkür

Bursa Uludağ Üniversitesi Bilimsel Araştırma Projeleri Birimine teşekkür ederiz.

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