HOW DOES QUERCETIN AND TAMOXIFEN AFFECT THE APOPTOSIS OF COLON CANCER CELLS?

Yıl 2015, Cilt: 37 Sayı: 2, 14 - 20, 25.12.2015

Didem Coşan Ahu Soyocak Ayşe Başaran İrfan Değirmenci Hasan Güneş Cengiz Bal

Öz

KOLON KANSER HÜCRELERİNİN APOPTOZUNU KERSETİN VE TAMOKSİFEN NASIL ETKİLER?

Özet: Diyetle alınan çeşitli sebze ve meyvelerde bulunan bileşiklerin kanser tedavisine yardımcı olabileceği bilinmektedir. Polifenolik bir bileşik olan kersetinin de bazı kanserlerde çeşitli moleküller üzerinden etkili olabildiği hakkında mevcut araştırmalar vardır. Ancak etki mekanizmalarının belirlenmesi için çalışmalar devam etmekte olup, tam olarak açıklığa kavuşmamıştır. Tamoksifen ise östrojen pozitif meme kanserinin tedavisinde kullanılan bir nonsteroid anti-östrojen ilaçtır. Zaman zaman meme kanseri dışında diğer bazı kanserlerde de kullanıldığı hakkında çeşitli bilgilere sahip olduğumuz tamoksifenin, kolon kanserinde de etkili olabileceğini düşünerek araştırmamızda kolon kanser hücrelerinde bu iki bileşenin apoptoz üzerine etkilerini incelemeyi amaçladık.  Kersetin ve tamoksifenin 24, 48 ve 72. saatlerde apoptotik indekse olan etkileri TUNEL metodu belirlendi. Kolon kanser hücrelerinde kersetin ve tamoksifenin çeşitli konsantrasyonlarda (25, 50 ve 100 µM) apoptotik indekse olan etkileri belirlendi. Bulgular: Apoptotik indeksin kersetin ve tamoksifen uygulanan gruplarda kontrol grubuna göre arttığı bulundu. Apoptotic index increased in groups that were administered quercetin and tamoxifen compared to control groups. Kolon adeno karsinomada kersetin ve tamoksifenin apoptozu indükleyerek kanser tedavisinde önemli bir rol oynayabileceğini bulunmuştur.

ANAHTAR KELİMELER: Apoptoz, CaCo-2, Kersetin, Kolon Kanseri, Tamoksifen

 

Abstarcat: It is a known fact that compounds found in various vegetables and fruits taken by diet may be of help in treating cancer. A polyphenolic compound, quercetin was reported to have a possible impact on some types of cancer through a variety of molecules. However, there are ongoing studies to prove the effect mechanisms and it has yet to be clarified. Tamoxifen is a non-steroidal anti-estrogen drug used in the treatment of estrogen-positive breast cancer. We already have some information regarding occasional use of tamoxifen in treatment of some cancers other than breast cancer, and thus we began investigating the effects of these two components on apoptosis in colon cancer cells in our study considering that it may also be effective in colon cancer. The effect of quercetin and tamoxifen on apoptotic index was evaluated by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method at the 24th, 48th, and 72nd hours. In colon cancer cells, the effects of various concentrations (25, 50, and 100 µM) of quercetin and tamoxifen on apoptotic index in colon cancer cells were established. Apoptotic index increased in groups that were administered quercetin and tamoxifen compared to control groups. The current study shows that quercetin and tamoxifen may play an important role in the treatment of cancer by inducing apoptosis in colon adenocarcinoma.

KEYWORD: Apoptosis, CaCo-2, Quercetin, Colon Cancer, Tamoxifen

Anahtar Kelimeler

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Kaynakça

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