SELENYUM SİKLOFOSFAMİD NEDENLİ HEPATOTOKSİSİTEYİ İYİLEŞTİREBİLİR

Yıl 2016, Cilt: 38 Sayı: 3, 34 - 39, 20.05.2016

Adnan Ayhancı Özge Acar Varol Şahintürk Sibel Güneş , İlknur Kulcanay Şahin Ahmet Musmul Sema Uslu

Öz

ÖZET: Siklofosfamid (CP)’in antitümör
etkinliği dozu ile doğru orantılıdır. Bununla birlikte, yüksek dozlarda yaygın
sitotoksisitelere neden olur. Selenyum (Se) yapısına katıldığı glutatyon
peroksidaz gibi selenoproteinler yoluyla potansiyel besinsel bir antioksidan
olarak biyolojik etkiler göstermektedir. Bu nedenle siklofosfamid nedenli
hepatotoksisitede selenyumun olası koruyucu etkisinin araştırılması
amaçlanmıştır. Toplam 42 adet erkek Spraque-Dawley sıçan 6 gruba (n=7) ayrıldı
(kontrol, 150 mg/kg CP, 0.5 ve 1 mg/kg Se, CP+0.5 ve CP+1 mg/kg Se grupları).
Se’un karaciğer toksisitesinde koruyucu etkisini belirlemek için serum alanin
transaminaz (ALT), aspartat transaminaz (AST), alkalen fosfataz (ALP) ve laktat
dehidrogenaz (LDH) seviyeleri belirlendi. Ayrıca karaciğer dokusu histolojik olarak
da incelendi. CP uygulanan grupta serum ALT (%145), AST (%226), ALP (%88), ve
LDH (%73) düzeyleri arttı. CP ile birlikte Se uygulanan gruplarda ALT, AST, ALP
ve LDH düzeyleri azaldı (p<0.05). Histolojik incelemelerde de CP+Se
gruplarında karaciğer doku hasarı önemli düzeyde azaldı. Sonuçlarımız
selenyumun antioksidan etkilerinin olduğu ve siklofosfamid nedenli oksidatif
hasarı ve çoklu organ toksisitelerini gidermede yararlı olabileceğini
göstermiştir.

ANAHTAR KELİMELER: Siklofosfamid, oksidatif stres, hepatotoksisite,
selenyum, rat.

SELENIUM AMELIORATES
CYCLOPHOSPHAMIDE-INDUCED HEPATOTOXICITY

ABSTRACT: The antitumoral efficieny of CP is
directly proportional to its administrated dose. However, high doses have a
tendency to result in generalized cytotoxicity. Selenium (Se) is a potent
nutritional antioxidant that carries out biological effects by its
incorporation into selenoproteins, such as glutathione peroxidase. Therefore we
aimed to investigate the possible protective effect of Se on CP-induced hepatotoxicity.
A total of 42 male Spraque-Dawley rats were divided into 6 groups (n=7)
(control, 150 mg/kg CP, 0.5 or 1 mg/kg Se and CP+0.5 and CP+1 mg/kg Se groups).
In order to determine the protective effects of Se on liver toxicity, the
levels of serum alanine transaminase (ALT), aspartate transaminase (AST),
alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were determined.
Also, the liver tissues were analysed histologically. Serum ALT (%145), AST
(%226), ALP (%88), and LDH (%73) levels increased in CP administrated rats. In
groups where CP and Se were given together ALT, AST, ALP, and LDH levels
decreased (p<0.05). Histological
analysis of liver tissue showed that tissue damage was significantly lower in
CP+Se groups. Our results show that Se has antioxidant effects and that it may
be useful to eliminate CP related oxidative damage.

KEYWORDS: Cyclophosphamide, oxidative stress,
hepatotoxicity, selenium, rat.

Anahtar Kelimeler

Siklofosfamid, oksidatif stres, hepatotoksisite, selenyum

Kaynakça

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