Evaluation of the Relationship of Paraoxonase 1 L55M and Q192R Polymorphism in Postmenopausal Osteoporosis

Year 2020, Volume: 42 Issue: 3, 289 - 295, 27.05.2020

Merih Özgen , Didem Turgut Coşan , Zeliha Atik Faruk Saydam Emine Çolak Ahu Soyocak Fulya Doğaner Funda Berkan , İrfan Değirmenci Fezan Mutlu

https://doi.org/10.20515/otd.534456

Abstract

Osteoporosis is
a systemic disease which is common in society, characterized by bone micro-architecture
deterioration and low bone mass. The aim of our study was to determine whether paraoxonase
1 (PON1) gene correlates with
584A>G (Gln192Arg) and 172T>A (Leu55Met) polymorphisms with
postmenopausal osteoporosis. A total of 143 postmenopausal osteoporosis and 102
healthy women who were followed up in Eskişehir Osmangazi University Faculty of
Medicine, Department of Physical Medicine and Rehabilitation were included in
the study. The study was planned retrospectively. Genotypes were identified on
the qPCR (The Applied Biosystems® StepOne ™) using the allele-specific DNA
primer and fluorescent-specific DNA probe. There was no statistically
significant difference between the groups in the genotype and allele
frequencies of PON1 584A>G
polymorphism (p=0.362 and p= 0.318). When the genotype and allele frequencies
of PON1 172T>A polymorphism were
compared, it was observed that T allele was significantly higher in
postmenopausal osteoporosis than healthy subjects (p <0.001, p <0.001,
respectively). PON1 172T>A (Leu55Met) gene
polymorphism was associated with postmenopausal osteoporosis in our study.

Keywords

Osteoporosis, PON1

Postmenopozal Osteoporozda Paraoksonaz 1 L55M ve Q192R Polimorfizminin İlişkisinin Değerlendirilmesi

Abstract

Osteoporoz,
toplumda yaygın görünen, kemik mikro mimarisinde bozulma ve düşük kemik kütlesi
ile karakterize sistemik bir hastalıktır. Çalışmamızın
amacı paraoksonaz (PON1) geninin
584A>G(Gln192Arg) ve 172T>A(Leu55Met) polimorfizmleri ile postmenopozal
osteoporoz ile ilişkisinin olup olmadığını saptamaktır. Eskişehir Osmangazi
Üniversitesi Tıp Fakültesi Fiziksel Tıp ve Rehabilitasyon Anabilim Dalı
Kliniği’nde takipli 143 postmenopozal osteoporozu olan ve 102 sağlıklı kadın
çalışmaya dahil edildi. Çalışma retrospektif olarak planlandı. Genotipler alel-spesifik
DNA primeri ve floresan özellikli DNA probu kullanılarak qPCR cihazında (The
Applied Biosystems® StepOne™) belirlendi.
PON1 584A>G polimorfizminin genotip ve alel
frekanslarında gruplar arasında istatistiksel olarak anlamlı bir fark
bulunmamıştır (p=0.362 ve p=0.318). PON1 172T>A polimorfizminin genotip ve alel frekansları
karşılaştırıldığında ise, sağlıklı bireylere göre postmenopozal osteoporozu
olanlarda T alelinin istatistiksel
olarak anlamlı derecede yüksek bulunduğu (sırasıyla p<0.001, p<0.001) gözlendi. Çalışmamızda PON1
172T>A(Leu55Met) gen polimorfizmi ile postmenopozal osteoporoz arasında
ilişki olduğu gösterildi. 

Keywords

Osteoporoz, PON1, Gen polimorfizm

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