Cerrahi Menopoz Oluşturulmuş Sıçanların Karaciğer ve Böbrek Dokularında Oksidan/Antioksidan Dengenin Korunmasında Egzersizin ve Östrojenin Yararlı Etkileri

Year 2022, , 460 - 467, 01.09.2022

Sevil Arabacı Tamer , Nişva Levent , Meral Yüksel , Feriha Ercan , Alper Yıldırım , Berrak Yeğen

https://doi.org/10.26453/otjhs.1080928

Abstract

Amaç: Bu çalışmanın amacı cerrahi olarak menopoz oluşturulan sıçanların böbrek ve karaciğerlerinde gözlenen histopatolojik ve fonksiyonel değişiklikleri ve östrojen veya egzersizin ya da östrojen-egzersiz kombinasyonunun oksidan hasar üzerine etkilerini araştırmaktır.

Materyal ve Metot: Anestezi altında Sprague Dawley dişi sıçanlara (n=32) bilateral overiektomi uygulandı ve tüm sıçanlar rastgele olarak iki gruba ayrıldı. Sıçanların yarısına normal içme suyu, diğer yarısının içme sularına östrojen (1mg/kg/gün) eklendi. İki hafta sonra gruplar kendi içlerinde sedanter ve egzersiz (5 gün/hafta, 30 dakika, 8 hafta) gruplarına ayrıldı. Deney protokolünün sonunda serum, karaciğer ve böbrek örnekleri biyokimyasal ve histopatolojik incelemeler için alındı. Femurda da histopatolojik değerlendirme yapıldı.

Bulgular: Cerrahi olarak menopoz oluşturulan sıçanlarda östrojenin böbrek dokusunda nötrofil infiltrasyonunu ve reaktif oksijen türlerinin üretimini baskılayarak koruyucu etki gösterdiği, kemik kütlesinde hafif düzeyde artışa neden olduğu, ancak karaciğerin antioksidan glutatyon düzeyinde azalmaya yol açtığı belirlenmiştir. Buna karşın, östrojen uygulaması menopozda yapılan egzersiz nedeniyle karaciğerde oluşan oksidan stresi engellemiştir. Egzersizle veya egzersize östrojen tedavisinin eklenmesiyle böbrek fonksiyonları önemli ölçüde etkilenmezken, kemik yapısında tek başına östrojene kıyasla daha olumlu değişiklikler gözlenmiştir.

Sonuç: Östrojen replasmanı kemik dokusundaki olumlu etkilerinin yanı sıra karaciğer ve böbrekte oksidan stresi azaltmakta ve özellikle karaciğerde egzersize bağlı gelişen oksidan stresi baskılayarak koruyucu etki göstermektedir.

Keywords

Egzersiz, menapoz, oksidan hasar, östrojen

Thanks

Deneyler sırasındaki desteklerinden dolayı Tülin Altınoluk, Miray Emran, Seda Korkmaz, Rozerin Göze Yüksel, Zeynep Baykal, Zehra Sena Dur'a teşekkür ederiz.

Beneficial Effects of Exercise and Estrogen in Maintaining Oxidant/Antioxidant Balance in the Liver and Kidney Tissues of Rats with Ovariectomy

Abstract

Objective: The aim of this study was to investigate the effects of estrogen or exercise or estrogen-exercise combination on oxidant damage and histopathological and functional changes observed in kidney and liver of rats with surgical menopause.

Materials and Methods: Bilateral ovariectomy was performed on female Sprague Dawley rats (n=32) under anesthesia and all rats were randomly divided into two groups. Half of the rats were given normal drinking water, and the other half had estrogen (1mg/kg/day) added to their drinking water. Two weeks later, the groups were divided into sedentary and exercise (5 days/week, 30 minutes, 8 weeks) groups. At the end of the experimental protocol, serum, liver and kidney samples were taken for biochemical and histopathological examinations. Femur was also examined histopathologically.

Results: In ovariectomized rats, estrogen showed a protective effect by suppressing neutrophil infiltration and production of reactive oxygen species in kidney tissue, causing a slight increase in bone mass, with a decrease in hepatic antioxidant glutathione level. On the other hand, estrogen application prevented the oxidant stress in the liver due to the exercise performed in menopause. While kidney functions were not significantly affected by exercise or the addition of estrogen therapy to exercise, more favorable changes were observed in bone structure compared to estrogen alone.

Conclusion: In addition to the positive effects of estrogen replacement on bone tissue, estrogen reduces oxidant stress in the liver and kidney and shows a protective effect by suppressing the oxidant stress that develops especially in the liver due to exercise.

Keywords

Exercise, menopause, oxidant damage, estrogen.

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