Sisplatin ve Karboplatin Yüklü Albumin Nanopartiküllerin Meme Kanseri Hücreleri Üzerindeki Sitotoksik Etkileri

Year 2023, , 115 - 121, 05.03.2023

Ferdane Danışman Kalındemirtaş , İshak Afşin Kariper

https://doi.org/10.26453/otjhs.1217364

Abstract

Amaç: Bu çalışmanın amacı kanser tedavisinde sıklıkla kullanılan karboplatin ve sisplatin ilaçlarının albümin nanotaşıyıcıya yüklenerek antikanser etkilerinin araştırılması ve karşılaştırılmasıdır.
Materyal ve Metot: Karboplatin (CP) ve Sisplatin (Cis) yüklenmiş albümin nanopartiküller, sırasıyla CP-NPs ve Cis-NPs olarak ultrasonikasyon kullanarak sentezlendi. Nanopartikül boyutu ve dağılımın homojenitesi Dinamik ışık saçılımı (DLS) ile değerlendirildi. Nanopartiküllerin sitotoksik aktiviteleri MDA-MB-231 ve MCF-7 meme kanseri hücrelerinde ve HUVEC hücrelerinde, MTT testi kullanılarak değerlendirildi ve morfolojik görüntüleri karşılaştırıldı.
Bulgular: CP-NPs’lerin boyutu ortalama 2-3 nm civarında iken, Cis-NPs’lerin 7-8 nm idi. Her iki nanopartikül grubunun da homojen bir şekilde dağıldığı görüldü. Sitotoksisite sonuçlarına göre CP-NPs ve Cis-NPs, MCF-7 meme kanseri hücrelerinde daha sitotoksikti. Ayrıca CP-NPs ve Cis-NPs’ler MDA-MB-231 meme kanseri hücrelerinde önemli sitotoksisite gösterirken, normal HUVEC hücrelerinde düşük sitotoksisite tespit edildi. CP-NPs ve Cis-NPs ile tedavi edilen MCF-7, tedavi edilmeyen MCF-7 ile karşılaştırıldı ve NPs'ler için istatistiksel anlamlılık P<0,01 olarak hesaplandı.
Sonuç: Meme kanseri hücrelerinde yüksek sitotoksisite gözlenirken, sağlıklı hücrelerde belirgin sitotoksisite gözlenmemiş olup albümine bağlı CP-NPs ve Cis-NPs kanser tedavisinde umut vadedici bir tedavi seçeneği olabilir.

Keywords

MCF-7, MDA-MB-231, meme kanseri, nano ilaç, sitotoksisite

Cytotoxic Effects of Cisplatin and Carboplatin Loaded Albumin Nanoparticles on Breast Cancer Cells

Abstract

Objective: This study aims to investigate and compare the anticancer effects of carboplatin and cisplatin, frequently used in cancer treatment, by loading them on albumin nanocarrier.
Materials and Methods: Carboplatin (CP) and Cisplatin (Cis) loaded albumin nanoparticles were synthesized using ultrasonication as CP-NPs and Cis-NPs, respectively. Nanoparticle size and distribution were evaluated by Dynamic light scattering (DLS). Cytotoxicities of NPs were evaluated in MDA-MB-231 and MCF-7 breast cancer cells and HUVEC using MTT test and their morphological images were compared.
Results: While the average size of CP-NPs was around 2-3 nm, Cis-NPs was 7-8 nm. It was observed that both NPs groups were homogeneously dispersed. According to the cytotoxicity results, both CP-NPs and Cis-NPs were more cytotoxic on MCF-7 breast cancer cells. In addition, CP-NPs and Cis-NPs showed significant cytotoxicity on MCF-7, MDA-MB-231 breast cancer cells, while low cytotoxicity was detected in normal HUVEC cells. The NPs treated MCF-7 was compared with the untreated MCF-7 and statistical significance was calculated as P<0.01 for CP-NPs and Cis-NPs.
Conclusions: Abumin-based CP-NPs and Cis-NPs showed high cytotoxicity in breast cancer cells, they have low cytotoxicity in healthy cells, making them promising for breast cancer treatment.

Keywords

Cytotoxicity, breast cancer, MCF-7, MDA-MB-231, nano drug

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