The Effect of Thiol-Disulfide Homeostasis on Prognosis in Patients with Early-Stage Non-Small Cell Lung Cancer

Year 2023, , 122 - 126, 05.03.2023

Murat Kavas , Cansel Atinkaya Baytemir , Murat Alışık , Akın Öztürk , Sümeyye Alparslan Bekir , Selma Aydoğan Eroğlu , Elçin Ersöz Köse , İrfan Yalçınkaya , Özcan Erel

https://doi.org/10.26453/otjhs.1226101

Abstract

Objective: Lung cancer has a poor prognosis. Thiol groups with high antioxidant capacity are converted to disulfide bonds through biochemical reactions that neutralize different oxidant compounds. The thiol-disulfide (SH-SS) homeostasis has significant effects on cell mechanisms, transcription, and apoptosis. Here we present the prognostic role of dynamic SH-SS homeostasis in patients operated for NSCLC.
Materials and Methods: Patients operating for early-stage NSCLC were prospectively analyzed. SH-SS homeostasis tests were measured using the automated spectrophotometric method.
Results: This study enrolled 138 subjects, including 77 patients and 61 healthy controls. Native thiol and total thiol levels were significantly lower in the patient group. The disulfide-to-native thiol ratio, which is an indicator of oxidative stress in SH-SS homeostasis, also reached a level of statistical significance in the patient group (p<0.001). According to the cut-off values (305 and 326.3), the median overall survival rate was significantly shorter in patients with low native thiol and total thiol levels (p<0.001).
Conclusions: This study demonstrated decreased native thiol and total thiol levels as well as decreased disulfide levels and SS/SH ratio in early-stage NSCLC. Impaired SH-SS homeostasis may contribute to lung cancer pathogenesis and poor prognosis because of enhanced oxidative stress.

Keywords

oxidative stress, thiol, disulphide, NSCLC, early stage lung cancer

Erken Evre Küçük Hücreli Dışı Akciğer Kanserli Hastalarda Tiyol-Disülfid Homeostazisin Prognoz Üzerine Etkisi

Abstract

Amaç: Akciğer kanseri kötü prognoza sahiptir. Antioksidan kapasitesi yüksek olan tiyol grupları, farklı oksidan bileşikleri nötralize eden biyokimyasal reaksiyonlarla disülfid (SS) gruplarına dönüşürler. Tiyol/Disülfid homeostazı (TDH) hücre mekanizmaları, transkripsiyon ve apoptoz üzerinde önemli etkilere sahiptir. KHDAK nedeniyle ameliyat edilen hastalarda dinamik TDH'nin prognozunun rolünü sunuyoruz.
Materyal ve Metot: KHDAK ile erken evre opere edilen hastalarda prospektif olarak analiz edildi. TDH testleri otomatik spektrofotometrik yöntemle ölçüldü.
Bulgular: Bu çalışmaya 77 hasta, 61 sağlıklı olmak üzere toplam 138 kişi katıldı. Native tiyol (NT) ve total tiyol (TT) düzeyleri hasta grubunda anlamlı olarak düşüktü. TDH'nin oksidatif stres göstergesi olan SS/NT oranı da hastalarda istatistiksel olarak anlamlı bulunmuştur (p<0,001). Medyan OS, NT ve TT düzeyi düşük olan hastalarda cut-off değerine göre (305 ve 326,3) anlamlı olarak daha kısaydı (p<0,001).
Sonuç: Bu sonuçlar erken evre KHDAK'de; doğal tiyol ve toplam tiyol seviyeleri azaldığını, disülfid seviyeleri ve disülfid/NT oranının da bozulduğunu göstermektedir. Bozulmuş tiyol/disülfid homeostazı, artan oksidatif stresin bir sonucu olarak akciğer kanseri patogenezine ve kötü prognoza katkıda bulunabilir.

Keywords

Disülfid, erken evre akciğer kanseri, KHDAK, oksidatif stres, tiyol

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