COVID-19 ile Nükleer Faktör Kappa B Genlerindeki Tek Nükleotid Polimorfizmleri Arasındaki Güçlü Bir İlişki

Abstract

Amaç: SARS-CoV-2, Betacoronavirus cinsine ait zarflı, pozitif polariteli, tek sarmallı bir RNA virüsüdür. Bu çalışmada COVID-19 hastalığının şiddeti ve NF-κB1 -94 ins/del (rs28362491), NF-κB1A 3’UTR A/G (rs696), NFk-B1A -826 C/T (rs2233406) polimorfizmleri arasındaki ilişkinin araştırılması amaçlanmıştır. Gereç ve Yöntem: COVID-19'lu 150 hastada ve kontrol olarak 171 sağlıklı bireyde bu genlerin polimorfizm sıklıklarını araştırdık. Kan örneklerinden total DNA izole edildi ve ardından genotipleme için PCR-RFLP çalışması kullanıldı. Tüm istatistiki analizler SPSS kullanılarak ki-kare yöntemi ile hesaplanmıştır. Bulgular: Rs28362491 polimorfizmi için D alel frekansı, WD ve DD genotip frekanslarında istatistiksel olarak anlamlı farklılıklar tespit edilmiştir. Rs696 polimorfizmi için, COVID-19'lu hastaların G aleli sıklığında istatistiksel olarak anlamlı bir fark vardı. Ayrıca, bu polimorfizm için CT ve TT genotip frekanslarının istatistiksel olarak anlamlı olduğu gösterilmiştir. Ayrıca, rs2233406 için T aleli, CT ve TT genotip frekanslarının istatistiksel olarak anlamlı bir farka sahip olduğu bulunmuştur. Sonuç: COVID-19 hastalığı ile NF-κB genleri arasında anlamlı bir ilişki bulunmuştur, ancak promotör aktivitesinin veya gen ekspresyon seviyelerinin araştırılması gibi daha ileri çalışmalara ihtiyaç vardır.

Keywords

• COVID-19
• NF-κB1-94 Ins/Del ATTG
• NF-κB1A 3
• NF-κB1A -826 C/T
• polimorifzm

Strong association between COVID-19 and Single Nucleotide Polymorphism in Nuclear Factor Kappa B genes

Abstract

Objectives: SARS-CoV-2 is an enveloped, positive-sense, single-stranded RNA virus belonging to the Betacoronavirus genus. This study aimed to investigate the relationship between COVID-19 severity and NF-κB1 -94 ins/del (rs28362491), NF-κB1A 3’UTR A/G (rs696), -826 C/T (rs2233406) polymorphisms. Materials and Methods: We investigated the frequencies of these gene polymorphisms in 150 patients with COVID-19 and 171 healthy controls. Total DNA was isolated from the blood samples, and then the PCR-RFLP study was used for genotyping. All statistical analyses were calculated using the chi-square method using SPSS. Results: A statistically significant differences were determined in the D allele frequency, WD and DD genotype frequencies for the rs28362491 polymorphism. For rs696 polymorphism, there was a statistically significant difference in the frequency of the G allele of patients with COVID-19. Additionally, for this polymorphism, CT and TT genotype frequencies were shown to be statistically significant. It was also found that the T allele, CT, and TT genotype frequencies for the rs2233406 have a statistically significant difference. Conclusion: A significant association was found between COVID-19 disease and NF-κB genes, but further studies, such as investigating promoter activity or gene expression levels, are needed.

Keywords

• COVID-19
• NF-κB1-94 Ins/Del ATTG
• NF-κB1A 3
• NF-κB1A -826 C/T
• polymorphism

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