Resveratrol Diyabet Modeli Oluşturulmuş ARPE-19 Hücrelerinin Bozulmuş Hücre Fonksiyonlarini Yeniden Düzenler

Abstract

Amaç: Yüksek kan şekeri seviyelerinin birçok görsel fonksiyona zarar verebileceği bilinmektedir. Bu çalışmada resveratrolün in vitro diyabet modeli oluşturulan ARPE-19 hücrelerinin hücresel lipid peroksidasyonu (MDA), sitokinler, VEGF-A ve apoptoz seviyeleri üzerindeki etkileri amaçlanmıştır. Materyal ve Metot: Altı deney grubu aşağıdaki şekilde oluşturulmuştur. 1-Kontrol grubu: Hiçbir tedavi uygulanmadı (Standart Büyütme Medyumu), 2-Mannitol Grubu (M): 19,5 mM mannitol takviyeli ortamda inkübe edilen hücreler, 3-Yüksek Glikoz Grubu (HG): Yüksek glikozda (25 mM Glikoz) inkübe edilen hücreler, 4-Resveratrol Grubu (R): Standart Büyütme Medyumunda ve 100 µM resveratrol ile inkübe edilen hücreler, 5-Mannitol + Resveratrol Grubu (M+R), 6-Yüksek Glikoz + Resveratrol Grubu (HG+R). Tüm gruplar 48 saat inkübasyon sonrasında MDA, IL-1β, TNF-α, VEGF-A ve Apoptozis düzeyleri ölçüldü. Bulgular: Yüksek glikoz ortamı ARPE-19 hücrelerinde MDA, IL-1β, TNF-α ve VEGF-A seviyelerinde artışa neden olurken resveratrol, diyabet modeli oluşturulmuş hücrelerde MDA, IL-1β, TNF-α ve VEGF-A seviyelerinde önemli bir azalmaya neden olduğu görülmüştür. Sonuç olarak resveratrolün ARPE-19 hücrelerinin diyabet modeline bağlı olarak bozulmuş hücresel fonksiyonların düzeltilmesine yardımcı olduğu belirlenmiştir. Sonuç: Resveratrol’ün hücresel oksidatif stresi azaltarak ve hücresel canlılığı destekleyerek bozulan hücresel fonksiyonları tersine çevirebileceği sonucuna varılmıştır.

Keywords

• Apoptoz
• ARPE-19
• diyabet
• resveratrol
• VEGF-A

Resveratrol Reorganizes the Impaired Cellular Functions of ARPE-19 Cells Created in Diabetes Model

Abstract

Objective: It is well known that high blood glucose levels can damage many visual functions. So, the study aimed to investigate the effects of resveratrol on cellular lipid peroxidation (MDA), cytokines, VEGF-A and apoptosis levels in vitro diabetes model-induced ARPE-19 cells. Materials and Methods: Six experimental groups were conceptualized as follows. 1-Control group: Received no treatment (Standard Growth Medium), 2-Mannitol Group (M): Cells incubated in 19.5 mM Mannitol supplemented medium, 3-High Glucose Group (HG): Cells incubated in high glucose (25 mM Glucose), 4-Resveratrol Group (R): Cells incubated with 100 µM resveratrol Standard Growth Medium, 5-Mannitol + Resveratrol Group (M+R), 6-High Glucose + Resveratrol Group (HG+R). In All groups, cells were incubated for 48 hrs, and MDA, IL-1β, TNF-α, VEGF-A and Apoptosis levels were measured. Results: High glucose medium increased the MDA, IL-1β, TNF-α and VEGF-A levels while resveratrol caused a significant decrement in MDA, IL-1β, TNF-α and VEGF-A levels in diabetes model-induced ARPE-19 cells. As a result, resveratrol prevented the ARPE-19 cells against diabetes related impaired conditions. Conclusions: In conclusion, resveratrol can reverse disrupted cellular functions by reducing cellular oxidative stress and supporting cellular viability.

Keywords

• Apoptosis
• ARPE-19
• diabetes
• resveratrol
• VEGF-A

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