Büyük adenomatöz kolorektal poliplerde insan papilloma virüs varlığının araştırılması

Öz

Kolorektal kanser dünya çapında en sık görülen malignitelerden biridir. Genellikle adenomatöz polip zemini üzerinde gelişir. İnsan papilloma virüsü (HPV), anogenital enfeksiyonlara neden olur ve serviks kanseri ile iyi bilinen bir ilişkiye sahiptir. Bununla birlikte, HPV ve kolorektal polip ve kanser arasındaki ilişkiyi araştıran çalışmalarda çelişkili sonuçlar vardır. Bu nedenle, kanser potansiyeli yüksek olan kolorektal adenomatöz poliplerde HPV varlığını değerlendirdik. Çalışmaya, alt gastrointestinal semptomların taranması veya değerlendirilmesi için total kolonoskopi uygulanan hastadan ≥1 cm boyutundaki polip alındı. Numuneler, real-time PCR kullanılarak 25 genotip için tarandı. Real-time PCR kullanılarak DNA izolasyon analizinde hiçbir örnekte HPV DNA saptanmadı. Sonuç: HPV ile adenomatöz kolorektal polipler arasında bir ilişkinin olamadığına  inanıyoruz. Bu konuyu açıklığa kavuşturmak için daha fazla çalışmaya ihtiyaç vardır.

Anahtar Kelimeler

• Human papilloma virus,Adenomatous polyps,Colorectal cancer

Investigation of presence of human papilloma virus in large adenomatous colorectal polyps

Abstract

Colorectal cancer is one of the most common malignancies worldwide. It often develops on grounds of adenomatous polyp. Human papilloma virus (HPV) causes anogenital infections and has well-known association with cervix cancer. However, there are conflicting results in the studies investigating relationship between HPV and colorectal polyps and cancer. Thus, we evaluated presence of HPV in colorectal adenomatous polyps with high potential for cancer. The study included polyps ≥1 cm in size from patient who underwent total colonoscopy for screening or evaluation of lower gastrointestinal symptoms. Samples were screened for 25 genotypes by using real-time PCR. No HPV DNA was detected in any of samples in DNA isolation assay by using real-time PCR. We believe that there is no association of colorectal adenomatous polyps with HPV. Further studies are needed to clarify this issue.

Keywords

• Human papilloma virus,Adenomatous polyps,Colorectal cancer

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