Hepatitis C Virus Genotype Distribution in Sakarya Province; Three-Year Retrospective Study

Year 2019, , 444 - 453, 31.12.2019

Semra Öz , Mehmet Köroğlu Ahmet Özbek Tayfur Demiray Oğuz Karabay Gülay Trak Mustafa Altındiş

https://doi.org/10.26453/otjhs.480164

Cited By: 2

Abstract

The
determination of HCV genotypes in the selection of antiviral therapy is helpful
in planning treatment and predicting response to treatment. The aim of this
study was to determine the HCV genotype distribution retrospectively. The study group consisted of 235
patients who were referred to Sakarya University Medical Microbiology
Laboratory for the determination of HCV genotype between January 2015 and
December 2017. The isolation of RNA from the samples was made by the
manufacturer with automatic isolation system (QIAsymphony, Qiagen, Germany). The study group; 105 (44.7%) males and
130 (55.3%) females, a total of 235 patients. Genotype distributions; 200 of
the samples (85.1%) were type 1, 2 (0.9%) type 2, 20 (8.5%) type 3, 7 (3.0%)
type 4 and 6 (2.6%) mix type (type 1a + type 1b). In genotype 1, 13 (5.5%) were
type 1a and 182 (77.4%) were type 1b. The prevalence of genotype 1b was
significantly higher in women than in men (p = 0.009). The mean age of patients
with genotype 1b was significantly higher than patients with other genotypes (p
<0.001). There was no significant change in the distribution of type 1b
according to years (p = 0.376). In our
study; Genotypes were found in the majority of the samples in our region and
type 1b in the subtype. Genotype 3 was the most common type after genotype 1.
These studies are expected to contribute significantly to the treatment of HCV
because of the guiding nature of the genotype in the selection of antiviral
treatment and clinical follow-up.

Keywords

Hepatitis C virus, genotype distribution, RNA

Sakarya İlinde Hepatit C Virüs Genotip Dağılımı; Üç Yıllık Retrospektif Çalışma

Abstract

HCV
genotiplerinin belirlenmesi antiviral tedavi seçiminde, tedavinin planlanması
ve tedaviye yanıtın öngörülmesinde yol göstericidir. Bu çalışmada retrospektif
olarak HCV genotip dağılımının belirlenmesi amaçlanmıştır. Çalışmada Ocak 2015-Aralık 2017 tarihleri arasında Sakarya
Üniversitesi Tıbbi Mikrobiyoloji Laboratuvarına HCV genotip tayini amacıyla
gönderilen 235 hastanın serum örneği çalışma grubunu oluşturdu. Örneklerden RNA
izolasyonu üretici firmanın önerileriyle otomatik izolasyon sistemi
(QIAsymphony, Qiagen, Almanya) ile yapıldı.
Çalışma grubu; 105 (%44,7) erkek, 130 (%55,3) kadın olmak üzere toplam 235
hastadan oluştu. Genotip dağılımları; örneklerden 200’ü (%85,1) tip 1, 2’si
(%0,9) tip 2, 20’si (%8,5) tip 3, 7’si (%3,0) tip 4 ve 6’sı (%2,6) mix tip (tip
1a+ tip 1b) idi. Genotip 1 içinde 13’ü (%5,5) tip 1a, 182’si (%77,4) tip 1b
iken, 5 (%2,1) örnekte alt tip belirlenemedi. Kadınlarda genotip 1b görülme
sıklığı erkeklere göre anlamlı olarak yüksekti (p=0,009). Genotip 1b saptanan
hastaların yaş ortalaması diğer genotiplere sahip hastalara göre anlamlı olarak
yüksekti (p<0,001). Yıllara göre tip1b dağılımında anlamlı bir değişiklik
saptanamadı (p=0,376). Sonuç olarak
çalışmamızda; bölgemizdeki örneklerin çoğunluğunda genotip 1, alt tipin ise tip
1b olduğu belirlenmiştir. Genotip 1’den sonra en sık, genotip 3 saptanmıştır.
Klinik sürecin takibi ve antiviral tedavi seçiminde genotip belirlenmesinin yol
gösterici olmasından dolayı bu çalışmaların HCV tedavisine önemli katkıları
olacağı düşünülmektedir.

Keywords

Hepatit C virüs, genotip dağılımı

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