Irak-Duhok'ta Petrol İstasyonların Çalışan İşçilerinin Mikronükleus Frekansı Üzerine Benzenin Etkisi

Öz

Amaç: Benzen başlıca organik ürünlerden biridir, bu organik ürüne kronik maruz kalan insanlar çeşitli sağlık sorunlarına maruz kalmaktadır. Bu çalışmanın amacı, Duhok eyaletindeki benzin istasyonu çalışanları arasında mesleki benzene maruz kalma ile ilişkili mikronükleus sıklığını değerlendirmektir.
Materyal ve Metot: Çalışma gruplarına mesleki olarak maruz kalan 25 benzin istasyonu çalışanı dahil edildi ve aynı sayıda yaş olarak eşleştirilmiş kontrol alındı ​​ve genetik hasar açısından değerlendirildi. Pul pul dökülmüş Buccal hücreleri her iki gruptan steril fırça kullanılarak toplandı. Etanol içinde sabitlenen ve Giemsa boyası ve 1000 hücre ile boyanmış lamlar bir ışık mikroskobu altında incelenmiştir. Student t ‑ testi ve P <0.05 ile yapılan veri yorumlaması için istatistiksel analiz istatistiksel olarak anlamlı kabul edildi. Bu çalışmada sigara ve alkol öyküsü olan hem kontrol hem de çalışanlar çalışma dışı bırakılmıştır.
Bulgular: Kontrol deneklerine kıyasla benzene maruz kalan işçilerde anlamlı düzeyde yüksek mikronükleus frekansı bulundu (sırasıyla 4,65 ± 2 ve 1,22 ± 7). Maruz kalma süresi mikronükleus frekansı üzerinde de anlamlı etkiler gösterirken (P <0.05), yaşın etkisi anlamlı değildi (P> 0.05).
Sonuçlar: Çalışmamızın sonuçları, benzenin maruz kalan deneklerde mikronükleus sıklığını arttırma potansiyeline sahip olduğu sonucuna varmıştır.

Anahtar Kelimeler

• : benzene,kanserojen,mikronükleus sıklığı,toksik ajanlar

The Effects of Benzene on Micronuclei Frequency of Workers in Petrol Stations in Duhok- Iraq

Abstract

Objective: Benzene is one of the major organic product, people with chronic exposure to this organic product are exposed to several health problems. The aim of this study was to evaluate the micronuclei frequency associated with occupational exposure to benzene among petrol station workers in Duhok Province.
Materials and Methods: The study groups included twenty-five occupationally exposed petrol station workers and twenty five age matched controls were recruited and evaluated for genetic damage. Exfoliated Buccal cells were collected from both groups by using the sterile brush. Slides fixed in ethanol and stained with Giemsa stain and 1000 cells were studied by using a light microscope. Statistical analysis for data interpretation performed by Student’s t‑test, and P < 0.05 was considered statistically significant. In this study, both Control and workers with history of Tobacco smoking and alcohol were excluded.
Results: As compared to control subjects, significant high level of micronuclei frequency was found in the workers exposed to benzene (4.65±2 and 1.22±7 respectively). Period of exposure also showed considerable effects (P<0.05) on micronuclei frequency, while effect of age was non-significant (P>0.05).
Conclusions: The results of our study concluded that benzene have the potential to increase micronuclei frequency in the exposed subjects. 

Keywords

• : benzene,micronuclei frequency,carcinogenic,toxic agents

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