Lomber Spinal Stenozun HLA İlişkisi Üzerine Ön Araştırma

Year 2020, , 683 - 692, 30.12.2020

Mustafa Efendioğlu , Ezgi Akar , Emine Koçman Recep Başaran , Gülbu Işıtmangil

https://doi.org/10.26453/otjhs.758530

Abstract

Amaç: Biz bu çalışmada lumbar spinal stenosis (LSS) ile genetik HLA sistemi arasındaki ilişkiyi tespit etmeyi amaçladık.

Materyal ve Metod: Bu çalışmada LSS tanısı konmuş 41 olgudan (7 erkek, 8 kadın; yaş ortalaması: 60,27) 15 inde HLA sınıf I ABC genlerini ve 41 olgunun tamamında (19 erkek, 22 kadın; yaş ortalaması: 60,46) HLA sınıf II DRDQB1 genlerini çalıştık. DNA ekstraksiyonundan sonra HLA-A, B, C, DQ ve DR’ye özgü primerlerle DNA amplifiye edildi ve SSP-PCR yöntemiyle çalışıldı. Allel tanımlamaları, resmi HLA Nomenclature Komitesine ait software ile yapıldı. 

Bulgular: LSS grubunda HLA A2 allel (%36,7) kontrol gruba (%17,2) göre istatistiksel anlamlı yüksekti (p:0,039; p<0,05). B44 allelinin görülme sıklığı (%20), kontrol grubundan (%5,1) istatistiksel olarak anlamlı yüksek tespit edildi. (p:0,010; p<0,05) Aynı şekilde C6 allelin görülme sıklığı (%26,7), kontrol grubundan (%8,6) istatistiksel anlamlı yüksek bulundu. (p:0,013; p<0,05). 

Sonuç: Bu ön çalışma LSS ile HLA sınıf I genlerinin ilişkili olduğunu ortaya koymuştur. HLA A2, HLA B44 ve HLA C6 alleleri LSS için predispozan faktörler olarak tespit edilmiştir.

Keywords

Human lökosit antijen, lomber spinal stenosis, sınıf I/II genler

Project Number

HNEAH KAEK 2019/16.

A Preliminary Research on HLA-Association of Lumbar Spinal Stenosis

Abstract

Objective: We aim to identify the relationship lumbar spinal stenosis (LSS) and genetic HLA system.
Materials and Methods: The present study investigates 15 cases out of 41 (7 male, 8 female; average age: 60.27) who were studied for HLA class I ABC genes, as well as totally 41 cases (19 male; 22 female; average age: 60.46) who were studied for HLA class II DR.DQB1 genes among the cases with LSS. DNA extraction, the DNA was amplified with primers specific to HLA-A, B, C, DQ and DR, and studied using the SSP-PCR method. Allele identification was made using the official HLA Nomenclature Committee software.
Results: HLA A2 allele in LSS group (%36.7) is statistically significantly higher than control group (%17.2) (p:0.039; p<0.05). The frequency of the B44 allele was statistically significantly higher in the LSS group (20%) than in the control group (5.1%) (p:0.010; p<0.05). Likewise, the frequency of the C6 allele was statistically significantly higher in the LSS group (26.7%) than in the control group (8.6%) (p:0.013; p<0.05).
Conclusion: This preliminary study has suggested that LSS is associated with HLA class I genes. HLA A2, HLA B44, and HLA C6 alleles have been determined as predisposing factors for LSS. 

Keywords

Class I/II genes, human leukocyte antigen, lumbar spinal stenosis

Project Number

HNEAH KAEK 2019/16.

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