Expression of Connexin 32 and Connexin 43 in Adenomatous Polyps and Hyperplastic Polyps

Abstract

Objective: Although connexins are thought to provide normally functioning gap junctions via a tumor suppressor gene, new studies point to an abnormal increase of connexins in various carcinomas and sarcomas. Therefore it was aimed to investigate the expression of connexin 43 (Cx43) and connexin 32 (Cx32) in adenomatous and hyperplastic polyps.

Materials and Methods: This retrospective study included 32 cases with adenomatous polyps and 42 cases with hyperplastic polyps, between 2014 and 2015. The samples were stained with immunohistochemical staining method with Cx43 and Cx32 and evaluated.

Results: In our study, we observed that Cx43 and Cx32 staining increased in hyperplastic polyps compared to adenomatous polyps (p=0.039, p=0.048 respectively). Statistically, there were no significant correlations between age, sex, location, and diameter with staining pattern.

Conclusion: Loss of Cx43 and Cx32 staining in adenomatous polyps was noted and thought to be related to dysplasia. 

Keywords

• Adenomatous polyps
• connexin 32
• connexin 43
• hyperplastic polyps

Adenomatöz ve Hiperplastik Poliplerde Konneksin 32 ve Konneksin 43 Ekspresyonu

Öz

Amaç: Konneksinlerin tümör baskılayıcı bir gen yoluyla normal işleyen geçit bağlantıları sağladığı düşünülse de yeni çalışmalar çeşitli karsinom ve sarkomlarda konneksinlerin anormal artışına işaret etmektedir. Bu nedenle adenomatöz ve hiperplastik poliplerde connexin 43 (Cx43) ve connexin 32 (Cx32) ekspresyonunun araştırılması amaçlandı.

Materyal ve Metot: Bu retrospektif çalışmaya 2014-2015 yılları arasında adenomatöz polipli 32 olgu ve hiperplastik polipli 42 olgu dahil edildi. Örnekler Cx43 ve Cx32 ile immünohistokimyasal boyama yöntemi ile boyandı ve değerlendirildi.

Bulgular: Çalışmamızda, hiperplastik poliplerde adenomatöz poliplere göre Cx43 ve Cx32 boyanmalarının arttığını gözlemledik (sırasıyla p=0,039, p=0,048). İstatistiksel olarak yaş, cinsiyet, yerleşim yeri ve çap ile boyama paterni arasında anlamlı bir ilişki yoktu.

Sonuç: Adenomatöz poliplerde Cx43 ve Cx32 boyanmasının azaldığı ve bu durumun displazi ile ilişkili olduğu düşünüldü. 

Anahtar Kelimeler

• Adenomatöz polipler
• hiperplastik polipler
• konneksin 32
• konneksin 43

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