The Significance of Netrin-1 Level in the Clinical Activity of Ulcerative Colitis, its Association between TNF-α and IL-6

Abstract

Objective: We investigated the importance of netrin-1 levels in Ulcerative Colitis (UC) in clinical activity of the disease, and its association with other proinflammatory cytokines IL-6 and TNF-alpha.

Materials and Methods: This study was a case-control study and included 67 patients with UC (36 activated, 31 in remission) and 50 healthy controls. UC patients were divided into mild activation (n=21), moderate activation (n= 6) and severe activation (n= 9) groups according to the "Truelove Witts clinical activity index". 31 asymptomatic patients were considered to be in remission. Netrin-1, IL-6 and TNF-alpha measurements in plasma samples were made using ELISA assay kit.

Results: Between the patient group and the control group; there was a statistically significant difference between netrin-1, IL-6, TNF-alpha, (p<0.05 for all). The plasma netrin-1 mean of UC with severe activation groupwas statistically significantly higher than that of the mild activation, remission group and control group (p<0.05). Plasma netrin-1 mean of UC with modarate activation group was statistically significantly higher than that of the mild activation and remission group.

Conclusion: We found that plasma netrin-1 levels increase with disease severity in UC, similar to proinflammatory cytokines such as TNF-alpha and IL-6.

Keywords

• Netrin-1
• Ulcerative Colitis
• IL-6
• TNF-alpha

Ülseratif Kolitli Hastalarda Netrin-1 Düzeyinin Klinik ve Hastalık Aktivitesini Değerlendirmedeki Rolü ve TNF-alfa, IL-6 ile İlişkisi

Öz

Amaç: Bu çalışmada Ülseratif Kolit (ÜK)'de netrin-1 düzeylerinin hastalığın klinik aktivitesindeki önemi ve diğer proinflamatuar sitokinlerden IL-6 ve TNF-alfa ile ilişkisini araştırdık.

Materyal ve Metot: Bu çalışma bir vaka control çalışması olup çalışmaya ÜK'li 67 hasta (36'sı aktivasyonlu, 31'i remisyonda) ve 50 sağlıklı kontrol dahil edildi. ÜK hastaları; "Truelove Witts klinik aktivite indeksi"ne göre hafif aktivasyon (n=21), orta aktivasyon (n=6) ve şiddetli aktivasyon (n=9) gruplarına ayrıldı. Asemptomatik olan 31 hasta Montreal sınıflandırılmasına göre remisyonda kabul edildi. Plazma örneklerindeki netrin-1, IL-6 ve TNF-alfa ölçümleri, ELİSA test kiti kullanılarak yapıldı.

Bulgular: Hasta grubu ile kontrol grubu arasında; netrin-1, IL-6 ve TNF-alfa düzeyleri arasında istatistiksel olarak anlamlı bir fark vardı (p<0,05). Şiddetli aktivasyon grubunda plazma netrin-1 düzeyi hafif şiddetli grup, remisyon grubu ve kontrol grubuna göre istatistiksel olarak daha yüksekti (p<0,05). ÜK grupları arasında TNF-alfa düzeyi istatistiksel olarak anlamlı bulunmazken (p>0,05), IL-6 düzeyi ise gruplar arasında anlamlı bulundu (p<0,05). Ayrıca netrin-1 ile IL-6 arasında pozitif yönde bir korelasyon saptanırken, netrin-1 ile TNF-alfa arasında korelasyon saptanmadı.

Sonuç: Çalışmamız; plazma netrin-1 düzeylerinin, TNF-alfa ve IL-6 gibi proinflamatuar sitokinlere benzer şekilde ÜK aktivasyonu ile ilişkili olduğunu ortaya koymaktadır. 

Anahtar Kelimeler

• Netrin-1
• Ülseratif Kolit
• TNF-alfa
• IL-6

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