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An Analysis of Plasma Thiol/Disulphide Homeostasis in Patients with Stable Angina

Year 2020, Volume 5, Issue 2, 318 - 323, 30.06.2020
https://doi.org/10.26453/otjhs.615454

Abstract

Objective: To investigate thiol/disulfide homeostasis in patients with stable angina pectoris.
Materials and Methods: The study population consisted of 85 stable angina pectoris patients and 31 healthy volunteers. To compare the thiol/disulfide values of the patients and controls, the newly developed analysis method was used by Erel and Neselioglu. Disulfide/total thiol, free thiol/total thiol and disulfide/free thiol values were calculated for both groups.
Results: Disulfide, free thiol and total thiol values obtained in the study were; 18.00 µmol/L, 365.08 µmol/L, 401.10 µmol/L for the patient group and 9.67 µmol/L for the control group, 372.51 µmol/L, 391.85 µmol/L for the control group. It is the first time thiol/disulphide homeostasis in plasma was examined by new developed method in stable angina pectoris patients with this study. Disulfide, disulfide/free thiol and disulfide/total thiol values were significantly higher in the patient group compared to the control group. free thiol/total thiol levels were significantly lower in patients (p<0.001).
Conclusion: Thiol and disulfide values can be seen at high levels in patients with stable angina pectoris, so thiol/disulfide homeostasis may be an indicator for this disease.

References

  • 1. Valgimigli M, Biscaglia S. Stable angina pectoris. Curr Atheroscler Rep. 2014;16(7):422. doi: 10.1007/s11883-014-0422-4
  • 2. Collet C, Capodanno D, Onuma Y, et al. Left main coronary artery disease: pathophysiology, diagnosis, and treatment. Nat Rev Cardiol. 2018;15(6):321-331.
  • 3. Bonomini F, Tengattini S. Atherosclerosis and oxidative stress. Histol Histopathol. 2008;23(3):381-390. doi: 10.14670/HH-23.381
  • 4. Dzau VJ, Antman EM, Black HR, et al. The cardiovascular disease continuum validated: clinical evidence of improved patient outcomes: part I: Pathophysiology and clinical trial evidence (risk factors through stable coronary artery disease). Circulation. 2006;114(25):2850-2870.
  • 5. Jones DP, Liang Y. Measuring the poise of thiol/disulfide couples in vivo. Free Radical Bio Med. 2009;47(10):1329-1338. doi: 10.1016/j.freeradbiomed.2009.08.021
  • 6. Winther JR, Thorpe C. Quantification of thiols and disulfides. BBA-Gen Subjects. 2014;1840 (2):838-846. doi:10.1016/j.bbagen.2013.03.031
  • 7. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014;47(18):326-332. doi:10.1016/j.clinbiochem.2014.09.026
  • 8. Ates I, Kaplan M, Yuksel M, et al. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine. 2016;51(1):47-51. doi: 10.1007/s12020-015-0784-6
  • 9. Ozler S, Erel O, Oztas E, et al. Serum thiol/disulphide homeostasis in preeclampsia. Hypertens Pregnancy. 2015;34 (4):474-485. doi: 10.3109/10641955.2015.1077859
  • 10. Erkus ME, Altiparmak IH, Akyuz AR, et al. The association between plasma thiol levels and left ventricular diastolic dysfunction in patient with hypertension. Scand J Clin Lab Inv. 2015;75(8):667-673.
  • 11. Ergin M, Cendek BD. Dynamic thiol-disulfide homeostasis in hyperemesis gravidarum. J Perinatol. 2015;35(10):788-792. doi: 10.1038/jp.2015.81
  • 12. Kundi H, Ates I, Kiziltunc E, et al. A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. Am J Emerg Med. 2015;33(11):1567-1571. doi: 10.1016/j.ajem.2015.06.016
  • 13. Ozyazici S, Karateke F, Turan U, et al. A novel oxidative stress mediator in acute appendicitis: Thiol/disulphidehomeostasis. Mediators Inflamm. 2016;10(2):1-6.
  • 14. Kayacan Y, Yazar H, Cerit G, Ghojebeigloo BE. A new oxidative stress indicator: Effect of 5-hydroxytryptophan on thiol-disulfide homeostasis in exercise. Nutrition. 2019;63(64):114-119. doi: 10.1016/j.nut.2019.01.013
  • 15. Silver MT, Rose GA, Paul SD, et al. A clinical rule to predict preserved left ventricular ejection fraction in patients after myocardial infarction. Ann intern med. 1994;121(10):750-756. doi:10.7326/0003-4819-121-10-199411150-00004
  • 16. Ellman G, Lysko H. A precise method for the determination of whole blood and plasma sulfhydryl groups. Anal Biochem. 1979;93(1):98-102.
  • 17. Ates I, Ozkayar N, Altay M, et al. Is disulphide/thiol ratio related to blood pressure in masked hypertension?. Clin Exp Hypertens. 2016;38(2):150-154.
  • 18. Yuvaci HU, Akdemir N, Bostanci MS, et al. Evaluation of the level of thiol-disulphide homeostasis in patients with mild and severe preeclampsia. Pregnancy Hypertens. 2016;6(4):394-399. doi: 10.1016/j.preghy.2016.09.003
  • 19. Elmas B, Erel Ö, Ersavaş D, Yürümez Y. Thiol/disulfide homeostasis as a novel indicator of oxidative stress in children with simple febrile seizures. Neurol Sci. 2017;38(11):1969-1975. doi: 10.1007/s10072-017-3087-2
  • 20. Kayacan Y, Yazar H, Kisa EC, Ghojebeigloo BE. A novel biomarker explaining the role of oxidative stress in exercise and l-tyrosine supplementation: thiol/disulphide homeostasis. Arch Physiol Biochem. 2018;124(3):232-236. doi: 10.1080/13813455.2017.1388410
  • 21. Dirican N, Dirican A, Sen O, et al. Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small cell lung cancer?. Redox Rep. 2016;21(5):197-203. doi: 10.1179/1351000215Y.0000000027
  • 22. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004;37(4):277-285. doi: 10.1016/j.clinbiochem.2003.11.015

Stabil Angina Pektoris Hastalarında Tiyol/Disülfid Homeostazı Analizi

Year 2020, Volume 5, Issue 2, 318 - 323, 30.06.2020
https://doi.org/10.26453/otjhs.615454

Abstract

Amaç: Stabil angina pektoris tanılı hastaların tiyol/disülfid homeostazını incelemektir.
Materyal ve Metot: Çalışma populasyonu 85 stabil angina pektoris hastası ile, 31 sağlıklı gönüllüden oluşmaktadır. Hasta ve kontrollerin tiyol/disülfid değerlerini karşılaştırmak için Erel ve Neşelioğlu tarafından yeni geliştirilen analiz metodu kullanılmıştır. Her iki grup için disülfid /total tiyol, serbest tiyol/total tiyol ve disülfid/serbest tiyol değerleri hesaplanmıştır. 
Bulgular: Çalışmada elde edilen disülfid, serbest tiyol ve total tiyol değerleri sırasıyla; hasta grubu için 18.00 µmol/L, 365,08 µmol/L, 401,10 µmol/L ve kontrol grubu için 9,67 µmol/L, 372,51 µmol/L, 391,85 µmol/L şeklinde bulunmuştur. Bu çalışma ile, stabil angina pektoris hastalarının tiyol/disulfid plazma değerleri yeni geliştirilen metot ile ilk defa belirlenmiştir. Disülfid, disülfid/serbest tiyol ve disülfid/total tiyol değerleri kontrole göre hasta grubunda anlamlı şekilde yüksek çıkarken; serbest tiyol/total tiyol değeri hastalarda anlamlı şekilde düşük çıkmıştır (p<0,001). 
Sonuç: Tiyol ve disülfit değerleri stabil anjina pektoris hastalarında yüksek düzeyde görülebilir, bu nedenle tiol/disülfid homeostazı bu hastalık için bir gösterge olabilir. 

References

  • 1. Valgimigli M, Biscaglia S. Stable angina pectoris. Curr Atheroscler Rep. 2014;16(7):422. doi: 10.1007/s11883-014-0422-4
  • 2. Collet C, Capodanno D, Onuma Y, et al. Left main coronary artery disease: pathophysiology, diagnosis, and treatment. Nat Rev Cardiol. 2018;15(6):321-331.
  • 3. Bonomini F, Tengattini S. Atherosclerosis and oxidative stress. Histol Histopathol. 2008;23(3):381-390. doi: 10.14670/HH-23.381
  • 4. Dzau VJ, Antman EM, Black HR, et al. The cardiovascular disease continuum validated: clinical evidence of improved patient outcomes: part I: Pathophysiology and clinical trial evidence (risk factors through stable coronary artery disease). Circulation. 2006;114(25):2850-2870.
  • 5. Jones DP, Liang Y. Measuring the poise of thiol/disulfide couples in vivo. Free Radical Bio Med. 2009;47(10):1329-1338. doi: 10.1016/j.freeradbiomed.2009.08.021
  • 6. Winther JR, Thorpe C. Quantification of thiols and disulfides. BBA-Gen Subjects. 2014;1840 (2):838-846. doi:10.1016/j.bbagen.2013.03.031
  • 7. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014;47(18):326-332. doi:10.1016/j.clinbiochem.2014.09.026
  • 8. Ates I, Kaplan M, Yuksel M, et al. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine. 2016;51(1):47-51. doi: 10.1007/s12020-015-0784-6
  • 9. Ozler S, Erel O, Oztas E, et al. Serum thiol/disulphide homeostasis in preeclampsia. Hypertens Pregnancy. 2015;34 (4):474-485. doi: 10.3109/10641955.2015.1077859
  • 10. Erkus ME, Altiparmak IH, Akyuz AR, et al. The association between plasma thiol levels and left ventricular diastolic dysfunction in patient with hypertension. Scand J Clin Lab Inv. 2015;75(8):667-673.
  • 11. Ergin M, Cendek BD. Dynamic thiol-disulfide homeostasis in hyperemesis gravidarum. J Perinatol. 2015;35(10):788-792. doi: 10.1038/jp.2015.81
  • 12. Kundi H, Ates I, Kiziltunc E, et al. A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. Am J Emerg Med. 2015;33(11):1567-1571. doi: 10.1016/j.ajem.2015.06.016
  • 13. Ozyazici S, Karateke F, Turan U, et al. A novel oxidative stress mediator in acute appendicitis: Thiol/disulphidehomeostasis. Mediators Inflamm. 2016;10(2):1-6.
  • 14. Kayacan Y, Yazar H, Cerit G, Ghojebeigloo BE. A new oxidative stress indicator: Effect of 5-hydroxytryptophan on thiol-disulfide homeostasis in exercise. Nutrition. 2019;63(64):114-119. doi: 10.1016/j.nut.2019.01.013
  • 15. Silver MT, Rose GA, Paul SD, et al. A clinical rule to predict preserved left ventricular ejection fraction in patients after myocardial infarction. Ann intern med. 1994;121(10):750-756. doi:10.7326/0003-4819-121-10-199411150-00004
  • 16. Ellman G, Lysko H. A precise method for the determination of whole blood and plasma sulfhydryl groups. Anal Biochem. 1979;93(1):98-102.
  • 17. Ates I, Ozkayar N, Altay M, et al. Is disulphide/thiol ratio related to blood pressure in masked hypertension?. Clin Exp Hypertens. 2016;38(2):150-154.
  • 18. Yuvaci HU, Akdemir N, Bostanci MS, et al. Evaluation of the level of thiol-disulphide homeostasis in patients with mild and severe preeclampsia. Pregnancy Hypertens. 2016;6(4):394-399. doi: 10.1016/j.preghy.2016.09.003
  • 19. Elmas B, Erel Ö, Ersavaş D, Yürümez Y. Thiol/disulfide homeostasis as a novel indicator of oxidative stress in children with simple febrile seizures. Neurol Sci. 2017;38(11):1969-1975. doi: 10.1007/s10072-017-3087-2
  • 20. Kayacan Y, Yazar H, Kisa EC, Ghojebeigloo BE. A novel biomarker explaining the role of oxidative stress in exercise and l-tyrosine supplementation: thiol/disulphide homeostasis. Arch Physiol Biochem. 2018;124(3):232-236. doi: 10.1080/13813455.2017.1388410
  • 21. Dirican N, Dirican A, Sen O, et al. Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small cell lung cancer?. Redox Rep. 2016;21(5):197-203. doi: 10.1179/1351000215Y.0000000027
  • 22. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004;37(4):277-285. doi: 10.1016/j.clinbiochem.2003.11.015

Details

Primary Language English
Subjects Health Care Sciences and Services
Journal Section Research article
Authors

Hayrullah YAZAR (Primary Author)
SAKARYA ÜNİVERSİTESİ, TIP FAKÜLTESİ
0000-0001-9447-6322
Türkiye


Esra YILDIZ This is me
University Faculty of Art and Sciences, Institute of Natural Sciences
0000-0001-8456-3502
Türkiye


Mustafa Baran İNCİ
İstanbul Halk Sağlığı Müdürlüğü
0000-0003-1893-5368
Türkiye


Mehmet Akif ÇAKAR
SAKARYA ÜNİVERSİTESİ, TIP FAKÜLTESİ
0000-0003-3216-4205
Türkiye


Betül ÖZBEK This is me
Ankara Şehir Hastanesi
0000-0003-3355-7801
Türkiye


Ceylan BAL
Ankara Şehir Hastanesi
0000-0002-1678-1281
Türkiye


Özcan EREL
Ankara Şehir Hastanesi
0000-0002-2996-3236
Türkiye

Publication Date June 30, 2020
Application Date September 4, 2019
Acceptance Date April 3, 2020
Published in Issue Year 2020, Volume 5, Issue 2

Cite

AMA Yazar H. , Yıldız E. , İnci M. B. , Çakar M. A. , Özbek B. , Bal C. , Erel Ö. An Analysis of Plasma Thiol/Disulphide Homeostasis in Patients with Stable Angina. OTJHS. 2020; 5(2): 318-323.