Sıçanlarda Amiodaronun Sebep Olduğu Akciğer Hasarında Beyaz Lahana Ekstraktının Koruyucu Rolü

Year 2022, Volume: 7 Issue: 1, 143 - 150, 01.03.2022

Esin Ak , İsmet Burcu Turkyilmaz , Aleyna Muhan Refiye Yanardağ

https://doi.org/10.26453/otjhs.1058146

Cited By: 2

Abstract

Amaç: Bu çalışmada amiodaron’un sebep olduğu akciğer hasarında beyaz lahananın koruyucu etkileri araştırılmıştır.
Materyal ve Metot: Bu çalışmada, sıçanlar 4 gruba ayrılmıştır. Kontrol grup: dokunulmamış sıçanlar, WCAE grup, sıçanlara 7 gün 500 mg/kg beyaz lahana ekstraktı verildi; AMD grup, sıçanlara 7 gün 100 mg/kg amiodaron (AMD) verildi; AMD+WCAE grup, sıçanlara aynı dozlarda beyaz lahana ekstraktı ve AMD verildi.
Bulgular: AMD+WCAE grubunda, beyaz lahana ekstraktı tedavisi interstisyal ödem ve konjesyonun azalmasına, alveolar yapılarda iyileşme ve bunun yanı sıra parankimdeki enflamatuvar hücre infiltrasyonun gerilemesine sebep olmuştur. Ek olarak, AMD+WCAE grubunda parankimde kollajen liflerinin birikmesinde önemli derecede bir azalma görülmüştür. Akciğerdeki glutatyon seviyesi, total antioksidan kapasitesi ve glutatyon-S-transferaz, paraoksonaz and karbonik anhidraz aktiviteleri azalırken, lipit peroksidasyon, ileri okside protein ürünleri, total oksidan durumu, reaktif oksijen türleri, oksidatif stres indeksi, nitrik oksit ve hidroksiprolin seviyeleri, katalaz, süperoksit dismutaz, glutatyon peroksidaz, glutatyon redüktaz, laktat dehidrogenaz and ksantin oksidaz aktiviteleri AMD grubunda artmıştır. Beyaz lahana ekstraktı tedavisi AMD’un neden olduğu bu seviyeleri ve aktiviteleri tersine çevirmiştir.
Sonuç: Beyaz lahana ekstraktının amiodaron’un sebep olduğu akciğer hasarını azaltabileceği sonucuna varabiliriz. 

Keywords

Akciğer, amiodaron, beyaz lahana ekstraktı, oksidatif stres

Protective Role of White Cabbage Extract Against Amiodarone-Induced Lung Damage in Rats

Abstract

Objective: It was intended to study the protective roles of white cabbage on amiodarone induced lung damage.
Materials and Methods: Rats were distributed into 4 groups, Control group, intact animals; WCAE group, animals given white cabbage extract (WCAE, 500 mg/kg) for 7 days; AMD group, animals administered amiodarone (AMD, 100 mg/kg) for 7 days; AMD+WCAE group, animals given white cabbage extract and amiodarone at the same dose.
Results: White cabbage extract treatment in AMD+WCAE group showed reduced interstitial edema and congestion, an improvement in alveolar structures besides regression of inflammatory cell infiltration in lung parenchyma. Moreover, a prominent reduction in the amount of collagen fibers deposition in the parenchyma was seen in AMD+WCAE group. Lung levels of glutathione and total antioxidant capacity and activities of glutathione-S-transferase, paraoxonase and carbonic anhydrase were decreased while the activities of lipid peroxidation, advanced oxidized protein products, total oxidant status, reactive oxygen species, oxidative stress index, nitric oxide and hydroxy proline levels, catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, lactate dehydrogenase and xanthine oxidase were increased in AMD group. Administration of white cabbage extract reversed these levels and activities in AMD group.
Conclusion: In conclusion, white cabbage extract can ameliorate amiodarone induced lung damage. 

Keywords

Amiodarone, lung, oxidative stress, white cabbage extract

References

• Lafuente-Lafuente C, Alvarez JC, Leenhardt A, et al. Amiodarone concentrations in plasma and fat tissue during chronic treatment and related toxicity. Br J Clin Pharmacol. 2009;67(5):511-519. doi:10.1111/j.1365-2125.2009.03381.x
• Al-Shammari B, Khalifa M, Bakheet SA, Yasser M. A Mechanistic study on the amiodarone-induced pulmonary toxicity. Oxid Med Cell Longev. 2016. doi:10.1155/2016/6265853
• Sarg NA, Kamal KM. Protection against lung toxicity induced by amiodarone in albino rats by fish oil. J Am Sci. 2019;15(12):82-92. doi:10.7537/marsjas151219.09
• Fouad GI, Mousa MR. The protective potential of alpha lipoic acid on amiodarone-induced pulmonary fibrosis and hepatic injury in rats. Mol Cell Biochem. 2021;476(9):3433-3448. doi:10.1007/s11010-021-04173-7
• Gado AM, Aldahmash BA. Protective effect of L-carnitine against amiodarone-induced lung toxicity in rats. Int J Toxicol. 2013;10 (1):1-9
• Vallejo F, Gil-Izquierdo A, Pérez-Vicente A, García-Viguera C. In vitro gastrointestinal digestion study of broccoli inflorescence phenolic compounds, glucosinolates, and vitamin. J Agric Food Chem. 2004;52(1):135–138. doi:10.1021/jf0305128
• Islam MA, Alam F, Solayman M, Khalil MI, Kamal MA, Gan SH. Dietary phytochemicals: Natural swords combating inflammation and oxidation-mediated degenerative diseases. Oxid Med Cell Longev. 2016. doi:10.1155/2016/5137431
• Koroglu-Aydın P, Bayrak BB, Bugan I, Karabulut-Bulan O, Yanardag R. Histological and biochemical investigation of the renoprotective effects of metformin in diabetic and prostate cancer model. Toxicol Mech Methods. 2021;31:489–500. doi:10.1080/15376516.2021.1919810
• Bayrak BB, Yilmaz S, Hacihasanoglu Cakmak N, Yanardag R. The effects of edaravone, a free-radical scavenger in lung injury induced by valproic acid demonstrated via different biochemical parameters. J Biochem Mol Toxicol. 2021;35(9):e22847. doi:10.1002/jbt.22847
• Turkyilmaz IB, Bilgin Sokmen B, Yanardag R. Alpha-lipoic acid prevents brain injury in rats administered with valproic acid. J Biochem Mol Toxicol. 2020;34(11):e22580. doi:10.1002/jbt.22580
• Papiris SA, Triantafillidou C, Kolilekas L, Markoulaki D, Manali ED. Amiodarone: review of pulmonary effects and toxicity. Drug Saf. 2010;33(7):539-558. doi:10.2165/11532320-000000000-00000
• Halliwell WH. Cationic amphiphilic drug-induced phospholipidosis. Toxicol Pathol. 1997;25:53-60. doi:10.1177/019262339702500111
• Varkhede NR, Jhajra S, Ahire DS, Singh S. Metabolite identification studies on amiodarone in in vitro (rat liver microsomes, rat and human liver S9 fractions) and in vivo (rat feces, urine, plasma) matrices by using liquid chromatography with high- resolution mass spectrometry and multiple-stage mass spectrometry: characterization of the diquinone metabolite supposedly responsible for the drug’s hepatotoxicity. Rapid Commun Mass Spectrom. 2014;28:311−331. doi:10.1002/rcm.6787
• Taylor MD, Antonini JM, Roberts JR, et al. Intratracheal amiodarone administration to F344 rats directly damages lung airway and parenchymal cells. Toxicol Appl Pharmacol. 2003;188:92-103. doi:10.1016/S0041-008X(02)00034-0
• Ott C, Jacobs K, Haucke E, Santos AN, Grune T, Simm A. Role of advanced glycation end products in cellular signaling. Redox Biol. 2014;2:411-429. doi:10.1016/j.redox.2013.12.016
• Oztay F, Tunali S, Kayalar O, Yanardag R. The protective effect of vitamin U on valproic acid-induced lung toxicity in rats via amelioration of oxidative stress. J Biochem Mol Toxicol.2020;34(12):e22602. doi:10.1002/jbt.22602
• Turkyilmaz IB, Yanardag R. Protecting effect of vitamin U against amiodarone-induced hepatic damage via its antioxidative activity. Bulg Chem Commun. 2019;51(1):20-24.
• Morales-López J, Centeno-Álvarez M, Nieto-Camacho A, et al. Evaluation of antioxidant and hepatoprotective effects of white cabbage essential oil. Pharm Biol. 2017;55:233-241. doi:10.1080/13880209.2016.1258424
• Veber B, Camargo A, Dalmagro AP, et al. Red cabbage (Brassica oleracea L.) extract reverses lipid oxidative stress in rats. An Acad Bras Cienc. 2020;92:e20180596. doi:10.1590/0001-3765202020180596
• Nicolescu AC, Ji Y, Comeau, JL, et al. Direct mitochondrial dysfunction precedes reactive oxygen species production in amiodarone-induced toxicity in human peripheral lung epithelial HPL1A cells. Toxicol Appl Pharmacol. 2008;227:370-379. doi:10.1016/j.taap.2007.12.009
• Choi WY, Park KY. Anticancer effects of organic Chinese cabbage kimchi. Int J Food Sci. 1999;4:113−116.
• Reiter RJ, Tan DX, Manchester LC, Qi W. Biochemical reactivity of melatonin with reactive oxygen and nitrogen species: A review of the evidence. Cell Biochem Biophys. 2001;34:237-256. doi:10.1385/CBB:34:2:237
• Kono H, Chen CJ, Ontiveros F, Rock KL. Uric acid promotes an acute inflammatory response to sterile cell death in mice. J Clin Investig. 2010;120:1939–1949. doi:10.1172/JCI40124
• Watanabe J, Kotani K, Gugliucci A. Paraoxonase 1 and chronic obstructive plmonary disease: A meta-analysis. Antioxidants (Basel). 2021;10(12):1891. doi:10.3390/antiox10121891
• Hazineci H, Türkyılmaz İB, Üstündağ ÜV, et al. 2020, White cabbage extract reduces the harmful effects of amiodarone on heart tissue of rats. Experimed. 2020;10:1-6.
• Kılınç N, Alim Z, Şengül B, İşgör MM, Beydemir Ş. Evaluation of inhibition effects of some cardiovascular therapeutics on human erythrocyte carbonic anhydrase isoenzymes. BEU J Sci. 2018;7(1):90-97. doi:10.17798/bitlisfen.345643
• Stravitz RT, Sanyal AJ. Drug-induced steatohepatitis. Clin Liver Dis. 2003;7(2):435-451. doi:10.1016/S1089-3261(03)00027-8
• Kim G, Choi HK, Lee HS, et al. Increased hepatic acylcarnitines after oral administration of amiodarone in rats. J Appl Toxicol. 2020;40(7):1004-1013. doi:10.1002/jat.3960