Diyabetik Sıçanlarda Hibiskus Ekstraktlarının Paraoxonase ve Antioksidan Aktiviteleri Üzerindeki Etkisinin Araştırılması

Year 2024, Volume: 9 Issue: 3, 246 - 253, 16.09.2024

Sedef Ziyanok Demirtaş

https://doi.org/10.26453/otjhs.1475794

Abstract

Amaç: Diyabetes mellitus (DM), metabolik işlevler üzerindeki geniş etkilerinin ötesinde, çeşitli kronik komplikasyonlarla bağlantılı olan oksidatif stresi hızlandırır. Bu çalışma, diyabetik sıçan modellerinde paraoksonaz (PON) ve arilesteraz enzim aktiviteleri üzerindeki etkilerine odaklanarak Hibiscus syriacus (HSE) ve Hibiscus trionum ekstraktlarının (HTE) antioksidatif ve terapötik potansiyelini araştırmaktadır.
Materyal ve Metot: Bu çalışma, kontrol (C), C+HSE, C+HTE, Diyabet (D), D+HSE ve D+HTE olmak üzere ayrılan 36 Wistar albino sıçanda PON ve ARE aktivitelerini değerlendirmiştir. HSE ve HTE'nin toplam fenolik içeriği Folin-Ciocalteu metodu ile belirlenmiş, antioksidan aktiviteleri DPPH ve CUPRAC testleri kullanılarak değerlendirilmiştir.
Sonuç: HSE ve HTE ekstraktları, diyabetik kardiyovasküler koruma ve oksidatif stresi azaltmada kritik öneme sahip olan paraoksonaz ve arilesteraz aktivitelerinde önemli artışlar göstermiştir. Sonuç: Bu çalışma, diyabetle ilişkili oksidatif stres komplikasyonlarının yönetiminde doğal ekstraktların potansiyelini vurgulamakta ve bu tür fitoterapötik ajanların geniş kapsamlı diyabet bakım stratejilerine entegre edilmesinin gerekliliğini ortaya koymaktadır. Gelecek çalışmalar, bu sonuçları klinik ortamlarda doğrulamayı ve gözlemlenen etkilerin altında yatan moleküler mekanizmaları araştırmayı hedeflemelidir, bu da diyabet yönetimi için yenilikçi müdahalelerin yolunu açabilir.

Keywords

Antioksidan aktivite, Diyabetes mellitus, Hibiscus syriacus, Hibiscus trionum, Paraoksonaz

Project Number

Bursa Uludag University (BUU) grant OAP(F)-2018/11.

Investigating the Impact of Hibiscus Extracts on Paraoxonase and Antioxidant Activities in Diabetic Rats

Abstract

Objective: Diabetes mellitus (DM) accelerates oxidative stress beyond its broad effects on metabolic function, which has been linked to various chronic complications. This study investigated the antioxidative and therapeutic potential of Hibiscus syriacus (HSE) and Hibiscus trionum extracts (HTE), focusing on their effects on paraoxonase (PON) and arylesterase enzymes activity in diabetic rat models.
Material and Methods: This study evaluated PON and ARE activities in 36 Wistar albino rats divided into the following groups: control (C), C+HSE, C+HTE, Diabetes (D), D+HSE, and D+HTE. The total phenolic content of HSE and HTE was determined using the Folin- Ciocalteu method, and their antioxidant activities were assessed using DPPH and CUPRAC tests.
Results: HSE and HTE extracts have demonstrated significant increases in paraoxonase and arylesterase activities, which are crucial for cardiovascular protection and reducing oxidative stress in diabetes.
Conclusions: This study highlights the potential of natural extracts in managing oxidative stress-related complications associated with diabetes and underscores the need to integrate such phytotherapeutic agents into broader diabetes care strategies. Future research should focus on confirming these findings in clinical settings and investigating the molecular processes responsible for the observed effects, potentially paving the way for innovative interventions for diabetes management.

Keywords

Antioxidant activity, Diabetes mellitus, Hibiscus syriacus, Hibiscus trionum, Paraoxonase

Ethical Statement

The animals used in this study were obtained with permission from the Bursa Uludag University Animal Experiments Local Ethics Committee (2018-06/07).

Supporting Institution

BUÜ

Project Number

Bursa Uludag University (BUU) grant OAP(F)-2018/11.

Thanks

I would like to express my gratitude to Prof. Dr. Ahmet Ilcim, who contributed to plant identification. I shall be forever grateful and extend my heartfelt thanks to Prof. Dr. Hulusi Malyer, who made highly appreciated contributions to the identification of the Hibiscus plant and very sadly passed away due to Covid-19. I extend my utmost thanks to Prof. Dr. Serap ÇELİKLER for her valuable support.

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