Biyosentetik gümüş nanopartiküllerinin Pyracantha coccinea bitkisinin gövde eksplantlarının yüzey sterilizasyonunda kullanımı

Year 2017, Volume: 23 Issue: 6, 759 - 761, 15.12.2017

Pınar Nartop

Abstract

Nanoteknoloji
günümüzde birçok alanda etkili olarak kullanılmaktadır. Gümüş nanopartiküllerin
de antibakteriyel ve antiseptik uygulamalarda sıklıkla kullanıldığı
görülmektedir. Gümüş nanopartiküllerin biyosentezi (yeşil sentez), fiziksel ve
kimyasal sentezlere alternatif olarak kullanılan bir yöntemdir. Bu çalışmada,
Alkanna tinctoria (havaciva) rizomlarının sulu ekstreleri gümüş nanopartikül
biyosentezinde kullanılmıştır. Kolloidal gümüş nanopartikül çözeltisi, Pyracantha coccinea (ateş dikeni) bitkisinin gövde eksplantlarının
yüzey sterilizasyonunda kullanılmış ve in vitro koşullardaki sterilizasyon
başarısı incelenmiştir. Elde edilen bulgulara göre, yüzeysel sterilizasyon
başarısında %10’luk NaOCl ve kolloidal gümüş nanopartikül çözeltisi
uygulamalarında istatistiksel olarak fark görülmemiştir. 20 dk. lık sterilan
uygulamasında ise gümüş nanopartiküller NaOCl ile aynı yüzeysel sterilizasyon
etkinliğini (%53.70) göstermiştir.

Keywords

Yeşil sentez, Gümüş nanopartiküller, Bitki eksplantlarının yüzeysel sterilizasyonu

Use of biosynthetic silver nanoparticles in the surface sterilization of Pyracantha coccinea stem explants

Abstract

Nanotechnology
is being used effectively in many areas contemporarily. Silver nanoparticles are
one of the most used materials in antibacterial and antiseptic practices.
Biosynthesis of silver nanoparticles

(green synthesis) is an alternative way to physical and chemical synthesis. In
this study, water extracts of Alkanna tinctoria (alkanet) rhizomes were used
for silver nanoparticle biosynthesis. Obtained colloidal silver nanoparticle solution
was applied to Pyracantha coccinea (scarlet
firethorn) stem explants for surface strerilization and the sterilization
ratios in in vitro conditions were inspected. With respect to data, difference
between 10% NaOCl and colloidal silver nanoparticle solution applications for
surface sterilization of the stems were not found statistically significant.
Moreover, in the application of sterilants for 20 minutes, silver nanoparticles
showed the same surface sterilization activity (53.70%) as NaOCl.

Keywords

Green synthesis, Silver nanoparticles, Surface sterilization of plant explants

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