Mikro boyutta pH temelli potansiyometrik glikoz biyosensör

Year 2018, Volume: 24 Issue: 7, 1355 - 1359, 28.12.2018

Derya Bal Altuntaş Özlem Tavukçuoğlu

Abstract

Günümüzdeki
bilimsel ve teknik ilerlemeler, biyosensör teknolojisinde önemli gelişmelere
yol açarak biyosensörlerin gıda teknolojileri, çevre, farmasi ve klinik teşhis,
biyokimya, analitik kimya gibi alanlarda yaygın olarak kullanılmasına neden
olmuştur. Potansiyometrik biyosensörlerin hazırlanmasında kullanılan
iyon-seçici sensör teknolojisindeki gelişmeler ve sensör materyallerin
sayılarının artması, yeni tip potansiyometrik biyosensörlerin geliştirilmesini
de hızlandırmıştır. Nitekim, bu çalışmada, kompozit pH sensörüne dayalı yeni
bir mikro boyutlu potansiyometrik glikoz biyosensörünün geliştirilmesi ele
alınmıştır. Glikoz oksidaz, biyotanıma elemanı olarak kompozit tabaka üzerinde
immobilize edildi. Kompozit pH algılama esaslı glikoz biyosensör matriksinin
yüzeyi ilk olarak elektrokimyasal tanımlama için dönüşümlü voltametri ve
elektrokimyasal impedans spektroskopisi kullanılarak incelendi. Yüksek elektron
transfer hızı, biyosensörün kararlı bir tepki davranışına sahip olduğunu ve
glikozun saptanması için uygun bir potansiyometrik sensör olduğunu
göstermektedir. Glikozun potansiyometrik tespiti için 2x10^-5 mol/L
saptama sınırı ile 5x10^-5 ila 1x10^-1 mol/ L konsantrasyon
aralığında lineer bir cevap elde edildi. Biyosensör hızlı bir tepki süresi (10
s) gösterdi, iyi bir kararlılığa ve uzun bir ömre sahipti.

Keywords

Katı-hal kompozit pH sensör, Minyatürizasyon, Diyabet takibi, Glikoz biyosensör

Micro-Sized glucose biosensor based on composite pH sensor

Abstract

Today's
scientific and technical advances have led to significant advances in biosensor
technology, leading to widespread use of biosensors in areas such as food
technology, the environment, pharmaceuticals and clinical diagnostics,
biochemistry, and analytical chemistry. Improvements in the ion-selective
sensor technology used in the preparation of potentiometric biosensors and the
increase in the number of sensor materials have also accelerated the
development of new types of potentiometric biosensors. Thus, in this study,
information is given on the development of a new micro sized potentiometric
glucose biosensor based on composite pH sensor. Glucose oxidase was immobilized
on the composite layer as the biorecognition element. The surface of glucose
biosensor based composite pH sensing matrices was first examined for
electrochemical elucidation by using cyclic voltammetry and electrochemical
impedance spectroscopy. The rate of electron transfer resulted in a stable
response behavior of the biosensor and made it a suitable potentiometric sensor
for detection of glucose. A linear response in concentration range from 5x10-⁵
to 1x10^-1 mol/L was obtained with a detection limit of 2x10^-5
mol/L for the potentiometric detection of glucose. The biosensor exhibited a
fast response time (10 s), had good stability, and an extended lifetime.

Keywords

Solid-state composite sensor, Miniatruization, Diabet follow-up, Glucose biosensor

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