Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet

Emine Kılıç Toprak; Melek Tunç Ata

doi:10.31362/patd.1398168

TR EN

Yüksek fruktozlu diyet ile metabolik sendrom oluşturulmuş sıçanlarda quercetin'in perirenal yağ dokusu adiponektin ve resistin düzeyleri üzerine etkisi

Abstract

Amaç: Metabolik sendrom (MetS) kalp hastalığı, inme ve tip 2 diyabet gelişimi için bir risk faktörleri kümesidir. Visseral adipozite ve insülin direnci MetS'in önemli mekanizmalarıdır. MetS’de gözlenen yağ dokusu artışı proinflamatuar ve antiinflamatuar sitokin dengesizliğine neden olur. MetS tedavisinde sıklıkla kullanılan antioksidanlardan biri olan quercetin hakkındaki kanıtlar, quercetin'in önemli anti-obezite ve lipid düşürücü etkilerinin eş zamanlı olduğu gösterilmiştir. Amacımız, yüksek fruktozla beslenen sıçanlarda quercetin takviyesinin MetS parametreleri ve yağ doku adipokin düzeyleri üzerindeki etkilerini araştırmaktır. Gereç ve yöntem: 8-10 haftalık Sprague Dawley sıçanlar, kontrol grubu (C), yüksek fruktoz (HF) grubu, quercetin (Q) grubu ve yüksek fruktoz+quercetin (HF+Q) grubu olmak üzere 4 gruba ayrıldı. Fruktoz, HF grup sıçanlarına içme suyunda %20'lik çözelti halinde 10 hafta süreyle uygulandı. Quercetin grubundaki sıçanlara deneyin son 4 haftasında vücut ağırlığı kg başına 50 mg quercetin gavaj yoluyla verildi. Sıçanlarda vücut ağırlığı, trigliserit (TG), yüksk dansiteli lipoprotein (HDL), açlık insülini, açlık glukozu ve HOMA-IR belirlendi. Adiponektin ve resistin seviyeleri perirenal yağ dokusu homojenatlarından ELISA yöntemi ile belirlendi. Bulgular: Yüksek fruktozla beslenen sıçanlarda quercetin'in TG'yi, açlık glikozunu ve insülin direncini iyileştirdiğini gösterdik. Bu çalışmada quercetin'in, perirenal yağ dokusu adiponektin ve resistin düzeylerine etkisi bulunmadı. Sonuç: Bu sonuçlar, yüksek fruktozun sıçanlarda MetS’i indükleyebileceğini, quercetin’in ise bu parametreleri olumlu yönde etkileyebileceğini göstermektedir.

Keywords

Metabolik sendrom, quercetin, perirenal adipoz doku, adiponektin, resistin

Project Number

2019HZDP015

Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet

Abstract

Purpose: Metabolic syndrome (MetS) is a cluster of risk factors for developing heart disease, stroke, and type 2 diabetes. Visceral adiposity and insulin resistance are crucial mechanisms of MetS. The increase in adipose tissue observed in MetS causes proinflammatory and anti-inflammatory cytokine imbalance. Accumulating evidence on quercetin, one of the antioxidants frequently used in MetS treatment, suggests that quercetin has significant anti-obesity and lipid-lowering effects simultaneously. Our aim is to investigate the effects of quercetin supplementation on MetS parameters and adipose tissue adipokine levels in rats fed high fructose. Materials and methods: Sprague Dawley rats, 8-10 weeks of age, were divided into 4 groups, including a control group (C), high fructose (HF) group, quercetin (Q) group, and high fructose+quercetin (HF+Q) group. Fructose was administered to HF groups as a 20% solution in drinking water for 10 weeks. The rats in the Q groups were given 50 mg quercetin per kg BW by gavage for the last 4 weeks of experiment. The body weight, triglyceride (TG), high density lipoprotein (HDL), fasting insulin, fasting glucose, and HOMA-IR were determined in rats. Adiponectin and resistin levels were determined by ELISA assay from perirenal adipose tissue homogenates. Results: We showed that quercetin acts to improve TG, fasting glucose and insulin resistance in high fructose-fed rats. In this study, we found no effect of quercetin on perirenal adipose tissue adiponectin and resistin levels. Conclusion: These results showed that high fructose could induce MetS in rats, while quercetin could favorably affect these parameters.

Keywords

Metabolic syndrome, quercetin, perirenal adipose tissue, adiponectin, resistin

Supporting Institution

Pamukkale Üniversitesi BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNATÖRLÜĞÜ

Project Number

2019HZDP015

Ethical Statement

" (PAUHDEK-2023/31)" konulu çalışmanız 19.10.2023 tarih ve 2023/06 sayılı toplantı

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other), Medical Physiology (Other)

Journal Section

Research Article

Authors

Emine Kılıç Toprak ^*
0000-0002-8795-0185
Türkiye

Melek Tunç Ata
0000-0002-0384-2356
Türkiye

Early Pub Date

March 21, 2024

Publication Date

April 1, 2024

Submission Date

November 30, 2023

Acceptance Date

March 15, 2024

Published in Issue

Year 2024 Volume: 17 Number: 2

DOI

https://doi.org/10.31362/patd.1398168

IZ

https://izlik.org/JA69WJ98UW

APA

Kılıç Toprak, E., & Tunç Ata, M. (2024). Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet. Pamukkale Medical Journal, 17(2), 347-357. https://doi.org/10.31362/patd.1398168

AMA

1.Kılıç Toprak E, Tunç Ata M. Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet. Pam Med J. 2024;17(2):347-357. doi:10.31362/patd.1398168

Chicago

Kılıç Toprak, Emine, and Melek Tunç Ata. 2024. “Effect of Quercetin on Perirenal Adipose Tissue Adiponectin and Resistin Levels in Rats With Metabolic Syndrome Induced by High Fructose-Diet”. Pamukkale Medical Journal 17 (2): 347-57. https://doi.org/10.31362/patd.1398168.

EndNote

Kılıç Toprak E, Tunç Ata M (April 1, 2024) Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet. Pamukkale Medical Journal 17 2 347–357.

IEEE

[1]E. Kılıç Toprak and M. Tunç Ata, “Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet”, Pam Med J, vol. 17, no. 2, pp. 347–357, Apr. 2024, doi: 10.31362/patd.1398168.

ISNAD

Kılıç Toprak, Emine - Tunç Ata, Melek. “Effect of Quercetin on Perirenal Adipose Tissue Adiponectin and Resistin Levels in Rats With Metabolic Syndrome Induced by High Fructose-Diet”. Pamukkale Medical Journal 17/2 (April 1, 2024): 347-357. https://doi.org/10.31362/patd.1398168.

JAMA

1.Kılıç Toprak E, Tunç Ata M. Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet. Pam Med J. 2024;17:347–357.

MLA

Kılıç Toprak, Emine, and Melek Tunç Ata. “Effect of Quercetin on Perirenal Adipose Tissue Adiponectin and Resistin Levels in Rats With Metabolic Syndrome Induced by High Fructose-Diet”. Pamukkale Medical Journal, vol. 17, no. 2, Apr. 2024, pp. 347-5, doi:10.31362/patd.1398168.

Vancouver

1.Emine Kılıç Toprak, Melek Tunç Ata. Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet. Pam Med J. 2024 Apr. 1;17(2):347-5. doi:10.31362/patd.1398168

Pamukkale Medical Journal is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY NC).

Yüksek fruktozlu diyet ile metabolik sendrom oluşturulmuş sıçanlarda quercetin'in perirenal yağ dokusu adiponektin ve resistin düzeyleri üzerine etkisi

Abstract

Keywords

Project Number

Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet

Abstract

Keywords

Supporting Institution

Project Number

Ethical Statement

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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