Yüksek fruktozlu diyet ile metabolik sendrom oluşturulmuş sıçanlarda quercetin'in perirenal yağ dokusu adiponektin ve resistin düzeyleri üzerine etkisi

Öz

Amaç: Metabolik sendrom (MetS) kalp hastalığı, inme ve tip 2 diyabet gelişimi için bir risk faktörleri kümesidir. Visseral adipozite ve insülin direnci MetS'in önemli mekanizmalarıdır. MetS’de gözlenen yağ dokusu artışı proinflamatuar ve antiinflamatuar sitokin dengesizliğine neden olur. MetS tedavisinde sıklıkla kullanılan antioksidanlardan biri olan quercetin hakkındaki kanıtlar, quercetin'in önemli anti-obezite ve lipid düşürücü etkilerinin eş zamanlı olduğu gösterilmiştir. Amacımız, yüksek fruktozla beslenen sıçanlarda quercetin takviyesinin MetS parametreleri ve yağ doku adipokin düzeyleri üzerindeki etkilerini araştırmaktır. Gereç ve yöntem: 8-10 haftalık Sprague Dawley sıçanlar, kontrol grubu (C), yüksek fruktoz (HF) grubu, quercetin (Q) grubu ve yüksek fruktoz+quercetin (HF+Q) grubu olmak üzere 4 gruba ayrıldı. Fruktoz, HF grup sıçanlarına içme suyunda %20'lik çözelti halinde 10 hafta süreyle uygulandı. Quercetin grubundaki sıçanlara deneyin son 4 haftasında vücut ağırlığı kg başına 50 mg quercetin gavaj yoluyla verildi. Sıçanlarda vücut ağırlığı, trigliserit (TG), yüksk dansiteli lipoprotein (HDL), açlık insülini, açlık glukozu ve HOMA-IR belirlendi. Adiponektin ve resistin seviyeleri perirenal yağ dokusu homojenatlarından ELISA yöntemi ile belirlendi. Bulgular: Yüksek fruktozla beslenen sıçanlarda quercetin'in TG'yi, açlık glikozunu ve insülin direncini iyileştirdiğini gösterdik. Bu çalışmada quercetin'in, perirenal yağ dokusu adiponektin ve resistin düzeylerine etkisi bulunmadı. Sonuç: Bu sonuçlar, yüksek fruktozun sıçanlarda MetS’i indükleyebileceğini, quercetin’in ise bu parametreleri olumlu yönde etkileyebileceğini göstermektedir.

Anahtar Kelimeler

• Metabolik sendrom
• quercetin
• perirenal adipoz doku
• adiponektin
• resistin

Effect of quercetin on perirenal adipose tissue adiponectin and resistin levels in rats with metabolic syndrome induced by high fructose-diet

Öz

Purpose: Metabolic syndrome (MetS) is a cluster of risk factors for developing heart disease, stroke, and type 2 diabetes. Visceral adiposity and insulin resistance are crucial mechanisms of MetS. The increase in adipose tissue observed in MetS causes proinflammatory and anti-inflammatory cytokine imbalance. Accumulating evidence on quercetin, one of the antioxidants frequently used in MetS treatment, suggests that quercetin has significant anti-obesity and lipid-lowering effects simultaneously. Our aim is to investigate the effects of quercetin supplementation on MetS parameters and adipose tissue adipokine levels in rats fed high fructose. Materials and methods: Sprague Dawley rats, 8-10 weeks of age, were divided into 4 groups, including a control group (C), high fructose (HF) group, quercetin (Q) group, and high fructose+quercetin (HF+Q) group. Fructose was administered to HF groups as a 20% solution in drinking water for 10 weeks. The rats in the Q groups were given 50 mg quercetin per kg BW by gavage for the last 4 weeks of experiment. The body weight, triglyceride (TG), high density lipoprotein (HDL), fasting insulin, fasting glucose, and HOMA-IR were determined in rats. Adiponectin and resistin levels were determined by ELISA assay from perirenal adipose tissue homogenates. Results: We showed that quercetin acts to improve TG, fasting glucose and insulin resistance in high fructose-fed rats. In this study, we found no effect of quercetin on perirenal adipose tissue adiponectin and resistin levels. Conclusion: These results showed that high fructose could induce MetS in rats, while quercetin could favorably affect these parameters.

Anahtar Kelimeler

• Metabolic syndrome
• quercetin
• perirenal adipose tissue
• adiponectin
• resistin

Kaynakça

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