Investigation of erythrocyte membrane lipid profile, oxidative stress and dna damage parameters in patients with chronic lymphoid leukemia
Abstract
Purpose: Chronic lymphocytic leukemia (CLL) is the most seen type of leukemia in adults. There are few biomarkes that are used for better understanding how oxidative stress is involved in the pathophysiology of hematologic malignency. We aimed to evaulate oxidative stress, DNA damage and erythrocyte membrane lipid profile in CLL patients in this study. The study is included 38 CLL patients and 38 age-sex matched controls.
Materials and method: Total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), DNA damage examination with Comet assay, serum 8-OHdG measurement and gas chromatographic analysis were performed between the case and control groups.
Results: It was observed that TOS and OSI values were higher in the case group than in the control group (p=0.014 and p=0.022, respectively). DNA damage measured by Comet method was found to be increased in the case group (p<0.05). Although erythrocyte membrane fatty acid levels were found to be decreased in the case group compared to the control group, no statistically significant difference was found (p>0.05).
Conclusion: It has been shown that CLL patients had higher oxidant capasity as consequence. Oxidative stress and DNA damage are increased in CLL patients in this study. It is early to evaulate on erythrocyte membrane lipid profile in CLL patients. However, the study can be ligthed the way future studies on the subject.
Keywords
Oxidative stress, DNA damage, erythrocyte membrane lipid profile, chronic lymphocytic leukemia
Supporting Institution
This study is supported by funding from the Pamukkale University (project number 2021TIPF027).
Project Number
This study is supported by funding from the Pamukkale University (project number 2021TIPF027).
Ethical Statement
Pamukkale University Faculty of Medicine Ethical Review Board approved this study (date: 31.05.2021, issue: 60116787-020-56364). We conducted all procedures involving human participants in accordance with the ethical standards of institutional and/or national research committees and the Declaration of Helsinki.
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