Kronik lenfoid lösemili hastalarda eritrosit membran lipid profili, oksidatif stres ve DNA hasarı parametrelerinin araştırılması

Öz

Amaç: Kronik lenfositik lösemi (KLL), erişkinlerde en sık görülen lösemi türüdür. Hematolojik malignite patofizyolojisinde oksidatif stresin nasıl rol oynadığını daha iyi anlamak için kullanılan az sayıda biyobelirteç vardır. Bu çalışmada KLL hastalarında oksidatif stres, DNA hasarı ve eritrosit membran lipit profilini değerlendirmeyi amaçladık. Çalışmaya 38 KLL hastası ve yaş-cinsiyet uyumlu 38 kontrol dahil edildi. Gereç ve yöntem: Olgu ve kontrol grupları arasında total oksidan durum (TOS), toplam antioksidan durum (TAS), oksidatif stres indeksi (OSI), Comet assay ile DNA hasarı incelemesi, serum 8-OHdG ölçümü ve gaz kromatografik analizi yapıldı. Bulgular: TOS ve OSI değerlerinin olgu grubunda kontrol grubuna göre daha yüksek olduğu görüldü (sırasıyla p=0,014 ve p=0,022). Comet yöntemiyle ölçülen DNA hasarının olgu grubunda arttığı belirlendi (p<0,05). Olgu grubunda eritrosit membran yağ asidi düzeylerinde kontrol grubuna göre azalma saptanmasına rağmen istatistiksel olarak anlamlı fark bulunamadı (p>0,05). Sonuç: Sonuç olarak KLL hastalarının oksidan kapasitesinin daha yüksek olduğu gösterilmiştir. Çalışmamızda KLL hastalarında oksidatif stres ve DNA hasarı artmıştır. KLL hastalarında eritrosit membran lipid profilini değerlendirmek için henüz erkendir. Ancak çalışmamız konuyla ilgili gelecekte yapılacak çalışmalara ışık tutabilir.

Anahtar Kelimeler

• Oksidatif stress
• DNA hasarı
• eritrosit membran lipid profili
• kronik lenfositik lösemi

Investigation of erythrocyte membrane lipid profile, oxidative stress and dna damage parameters in patients with chronic lymphoid leukemia

Öz

Purpose: Chronic lymphocytic leukemia (CLL) is the most seen type of leukemia in adults. There are few biomarkes that are used for better understanding how oxidative stress is involved in the pathophysiology of hematologic malignency. We aimed to evaulate oxidative stress, DNA damage and erythrocyte membrane lipid profile in CLL patients in this study. The study is included 38 CLL patients and 38 age-sex matched controls. Materials and method: Total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), DNA damage examination with Comet assay, serum 8-OHdG measurement and gas chromatographic analysis were performed between the case and control groups. Results: It was observed that TOS and OSI values were higher in the case group than in the control group (p=0.014 and p=0.022, respectively). DNA damage measured by Comet method was found to be increased in the case group (p<0.05). Although erythrocyte membrane fatty acid levels were found to be decreased in the case group compared to the control group, no statistically significant difference was found (p>0.05). Conclusion: It has been shown that CLL patients had higher oxidant capasity as consequence. Oxidative stress and DNA damage are increased in CLL patients in this study. It is early to evaulate on erythrocyte membrane lipid profile in CLL patients. However, the study can be ligthed the way future studies on the subject.

Anahtar Kelimeler

• Oxidative stress
• DNA damage
• erythrocyte membrane lipid profile
• chronic lymphocytic leukemia

Kaynakça

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