Selenyumun sıçan hipokampusunda cisplatin kaynaklı oksidatif hasara karşı nöroprotektif etkileri

Öz

Amaç: Bu çalışma, selenyumun cisplatin kaynaklı merkezi sinir sistemi nörotoksisitesine karşı potansiyel bir nöroprotektif ajan olarak rolünü değerlendirmek amacıyla, oksidatif stresin hipokampüsteki rolüyle birlikte bilişsel ve davranışsal etkileri kapsamlı bir şekilde değerlendirmeyi amaçlamıştır. Gereç ve yöntem: Otuz sekiz erkek sıçan kontrol, sisplatin, selenyum ve sisplatin+selenyum grublarına ayrıldı. Sıçanlara tek doz sisplatin (7,5 mg/kg) intraperitoneal olarak uygulandı. Selenyum (1 mg/kg) 21 gün boyunca günde bir kez oral yoldan gavaj yoluyla uygulandı. Kısa süreli hafızayı (KSH) ve uzun süreli hafızayı (USH) test etmek için yeni konum ve nesne tanıma testleri kullanıldı. Depresyon düzeyini ölçmek için zorunlu yüzme testi kullanıldı. Toplam antioksidan durumu (TAS), süper oksidaz dismutaz (SOD), toplam oksidan durumu (TOS) ve malondialdehit (MDA), ELISA yöntemi kullanılarak hipokampüs dokusundan ölçüldü. Bulgular: Cisplatin tedavisi sıçanlarda kilo kaybına neden oldu p=0,001. Cisplatin grubunda kontrol grubuna göre TOS p=0,02 ve MDA p=0,05 seviyelerinde artış, TAS p=0,03 ve SOD p=0,01 seviyelerinde azalma görüldü. Cisplatin+Selenyum grubunda bu oksidatif hasar parametrelerinde kontrol grubuna kıyasla istatistiksel olarak anlamlı bir değişiklik gözlenmedi. Cisplatin grubunda KSH ve USH'de bozulma görüldü p=0,05. Ancak Cisplatin+Selenyum grubunda USH'daki bozulmada bir miktar iyileşme görüldü. Cisplatin grubunda, kontrol grubuna kıyasla depresyon düzeyinde artış gözlemlenirken, Cisplatin+Selenyum grubunda önemli ölçüde azaldı p=0,001. Sonuç: Elde edilen bulgular, selenyum takviyesinin sıçanlarda cisplatin kaynaklı merkezi sinir sisteminde nörotoksik etkilerin hafifletilmesinde potansiyel terapötik bir rol oynayabileceğini düşündürmektedir.

Anahtar Kelimeler

• Hipokampus
• selenyum
• oksidatif stres
• davranışsal test

Neuroprotective effects of selenium against cisplatin-induced oxidative damage in the rat hippocampus

Abstract

Purpose: This study aimed to comprehensively evaluate the cognitive and behavioral effects together with the role of oxidative stress in the hippocampus in order to evaluate the role of selenium as a potential neuroprotective agent against cisplatin-induced central nervous system neurotoxicity. Materials and methods: Thirty-eight male rats were divided into control, cisplatin, selenium, and cisplatin+selenium groups. A single dose of cisplatin (7.5 mg/kg) was administered intraperitoneally to the rats. Selenium (1 mg/kg) was administered orally once daily by gavage for 21 days. Novel location and object recognition tests were used to test short-term memory (STM) and long-term memory (LTM). The forced swim test was used to measure depression level. Total antioxidant status (TAS), superoxide dismutase (SOD), total oxidant status (TOS), and malondialdehyde (MDA) were measured in hippocampus tissue using the ELISA method. Results: Cisplatin treatment caused weight loss in rats p=0.001. TOS p=0.02 and MDA p=0.05 levels increased, and TAS p=0.03 and SOD p=0.01 levels decreased in the cisplatin group compared to the control group. No statistically significant change was observed in these oxidative damage parameters in the cisplatin+selenium group compared to the control group. STM and LTM were impaired in the cisplatin group p=0.05. However, some improvement was observed in the LTM impairment in the Cisplatin+selenium group. While depression levels increased in the cisplatin group compared to the control group, they were significantly reduced in the Cisplatin+selenium group p=0.001. Conclusion: The findings suggest that selenium supplementation may play a potential therapeutic role in alleviating cisplatin-induced neurotoxic effects in the central nervous system in rats.

Keywords

• Hippocampus
• selenium
• oxidative stress
• behavioral test

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