Dual manyetik alan tedavisi, sıçanlarda LPS ile indüklenen pankreas hasarını, adacık yapısını ve endokrin fonksiyonu koruyarak azaltır

Abstract

Amaç: Sepsis, pankreas disfonksiyonu da dahil olmak üzere çoklu organ yetmezliğine yol açabilen, yaşamı tehdit eden sistemik bir inflamatuar yanıttır. Bu çalışmanın amacı, lipopolisakkarit (LPS) ile indüklenen pankreas hasarı üzerinde radyo frekans (RF) ve darbeli manyetik alan (PM) tedavilerinin koruyucu etkilerini değerlendirmektir. Gereç ve yöntem: Kırk dişi Wistar albino rat Kontrol, LPS, LPS+RF, LPS+PM ve LPS+PM+RF olmak üzere rastgele beş gruba ayrıldı (n=8). LPS, intraperitoneal olarak (5 mg/kg) uygulandı, ardından RF (30 dakika) ve/veya PM (3 saat) uygulamaları yapıldı. Pankreas dokuları histopatolojik olarak incelendi ve immünohistokimyasal olarak insülin, glukagon ve amilin ekspresyonları değerlendirildi. Bulgular: LPS uygulaması sonucunda pankreaslarda hiperemi, ödem ve inflamatuar infiltrasyon gibi belirgin histopatolojik hasarlar gözlendi. Ayrıca adacık hücrelerinde insülin ve amilin ekspresyonları azaldığı, glukagon ekspresyonu ise arttığı dikkati çekti. Hem RF hem de PM tedavilerinin bu değişiklikleri hafiflettiği saptandı. Özellikle PM+RF kombinasyonu, normal histolojik yapının ve hormonal expresyonların en belirgin şekilde düzeltilmesini sağladı. Sonuç: RF ve PM tedavileri, özellikle birlikte uygulandıklarında, sepsise bağlı pankreas hasarına karşı önemli koruyucu etkiler göstermektedir. Bu etkiler, inflamasyonu azaltma ve adacık hücre fonksiyonlarını koruma yoluyla gerçekleşmektedir. Bulgular, inflamatuar durumlarda pankreas sağlığını desteklemek için farmakolojik olmayan yeni bir yaklaşımı ortaya koymaktadır.

Keywords

• Sepsis
• pankreas
• radyo frekans
• darbeli manyetik alan
• LPS

Supporting Institution

Bu çalışma Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından TSG-2024-9515 proje kodu ile desteklenmiştir.

Ethical Statement

10/222 1/11/2023 tarihli karar Süleyman Demirel Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu

Thanks

Yazarlar, destekleri için Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi'ne ve doku örneklerini sağladığı için Dr. M. Soner Özcan'a teşekkür eder.

Dual magnetic field therapy mitigates LPS-induced pancreatic injury by preserving islet structure and endocrine function in rats

Abstract

Purpose: Sepsis is a life-threatening systemic inflammatory response that can lead to multiorgan failure, including pancreatic dysfunction. This study aimed to investigate the potential protective effects of radiofrequency (RF) and pulsed magnetic field (PM) therapies against lipopolysaccharide (LPS)-induced pancreatic injury in rats. Materials and methods: Forty female Wistar albino rats were randomly assigned to five groups (n=8 per group): Control, LPS, LPS + RF, LPS + PM, and LPS + PM + RF. LPS was administered intraperitoneally (5 mg/kg), followed by RF (30 minutes) and/or PM (3 hours) exposure. Pancreatic tissues were examined histopathologically and immunohistochemically for insulin, glucagon, and amylin expression. Results: LPS administration resulted in significant histopathological damage, including hyperemia, edema, and inflammatory infiltration, as well as reduced insulin and amylin immunopositivity and increased glucagon expression in islets. Both RF and PM treatments alleviated these changes, with the combined PM+RF application showing the most prominent protective effects, restoring normal histological architecture and hormonal profiles. Conclusion: RF and PM therapies, particularly in combination, exhibit significant protective effects against sepsis-induced pancreatic injury by mitigating inflammation and preserving islet cell function. These findings highlight a novel non-pharmacological approach for supporting pancreatic health in inflammatory conditions.

Keywords

• Sepsis
• pancreas
• radiofrequency
• pulsed magnetic field
• LPS

Supporting Institution

The authors disclosed receipt of the following financial support for the research. This study was supported by the Scientific Research Projects Coordination Unit of Süleyman Demirel University with project code TSG-2024-9515.

Ethical Statement

10/222 decision dated 1/11/2023 Süleyman Demirel University Animal Experiments Local Ethics Committee

Thanks

The authors would like to thank the Scientific Research Projects Coordination Unit of Süleyman Demirel University for their support and Dr. M. Soner Özcan for providing the tissue samples.

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