Dual manyetik alan tedavisi, sıçanlarda LPS ile indüklenen pankreas hasarını, adacık yapısını ve endokrin fonksiyonu koruyarak azaltır

Öz

Amaç: Sepsis, pankreas disfonksiyonu da dahil olmak üzere çoklu organ yetmezliğine yol açabilen, yaşamı tehdit eden sistemik bir inflamatuar yanıttır. Bu çalışmanın amacı, lipopolisakkarit (LPS) ile indüklenen pankreas hasarı üzerinde radyo frekans (RF) ve darbeli manyetik alan (PM) tedavilerinin koruyucu etkilerini değerlendirmektir. Gereç ve yöntem: Kırk dişi Wistar albino rat Kontrol, LPS, LPS+RF, LPS+PM ve LPS+PM+RF olmak üzere rastgele beş gruba ayrıldı (n=8). LPS, intraperitoneal olarak (5 mg/kg) uygulandı, ardından RF (30 dakika) ve/veya PM (3 saat) uygulamaları yapıldı. Pankreas dokuları histopatolojik olarak incelendi ve immünohistokimyasal olarak insülin, glukagon ve amilin ekspresyonları değerlendirildi. Bulgular: LPS uygulaması sonucunda pankreaslarda hiperemi, ödem ve inflamatuar infiltrasyon gibi belirgin histopatolojik hasarlar gözlendi. Ayrıca adacık hücrelerinde insülin ve amilin ekspresyonları azaldığı, glukagon ekspresyonu ise arttığı dikkati çekti. Hem RF hem de PM tedavilerinin bu değişiklikleri hafiflettiği saptandı. Özellikle PM+RF kombinasyonu, normal histolojik yapının ve hormonal expresyonların en belirgin şekilde düzeltilmesini sağladı. Sonuç: RF ve PM tedavileri, özellikle birlikte uygulandıklarında, sepsise bağlı pankreas hasarına karşı önemli koruyucu etkiler göstermektedir. Bu etkiler, inflamasyonu azaltma ve adacık hücre fonksiyonlarını koruma yoluyla gerçekleşmektedir. Bulgular, inflamatuar durumlarda pankreas sağlığını desteklemek için farmakolojik olmayan yeni bir yaklaşımı ortaya koymaktadır.

Anahtar Kelimeler

• Sepsis
• pankreas
• radyo frekans
• darbeli manyetik alan
• LPS

Dual magnetic field therapy mitigates LPS-induced pancreatic injury by preserving islet structure and endocrine function in rats

Öz

Purpose: Sepsis is a life-threatening systemic inflammatory response that can lead to multiorgan failure, including pancreatic dysfunction. This study aimed to investigate the potential protective effects of radiofrequency (RF) and pulsed magnetic field (PM) therapies against lipopolysaccharide (LPS)-induced pancreatic injury in rats. Materials and methods: Forty female Wistar albino rats were randomly assigned to five groups (n=8 per group): Control, LPS, LPS + RF, LPS + PM, and LPS + PM + RF. LPS was administered intraperitoneally (5 mg/kg), followed by RF (30 minutes) and/or PM (3 hours) exposure. Pancreatic tissues were examined histopathologically and immunohistochemically for insulin, glucagon, and amylin expression. Results: LPS administration resulted in significant histopathological damage, including hyperemia, edema, and inflammatory infiltration, as well as reduced insulin and amylin immunopositivity and increased glucagon expression in islets. Both RF and PM treatments alleviated these changes, with the combined PM+RF application showing the most prominent protective effects, restoring normal histological architecture and hormonal profiles. Conclusion: RF and PM therapies, particularly in combination, exhibit significant protective effects against sepsis-induced pancreatic injury by mitigating inflammation and preserving islet cell function. These findings highlight a novel non-pharmacological approach for supporting pancreatic health in inflammatory conditions.

Anahtar Kelimeler

• Sepsis
• pancreas
• radiofrequency
• pulsed magnetic field
• LPS

Kaynakça

1. Spapen H. Liver perfusion in sepsis, septic shock, and multiorgan failure. Anat Rec (Hoboken). 2008;291(6):714-720. doi:10.1002/ar.20646
2. Li Y, Suo L, Zhang J. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2020;32(4):504-507. doi:10.3760/cma.j.cn121430-20191021-00063
3. Virzì GM, Mattiotti M, de Cal M, Ronco C, Zanella M, De Rosa S. Endotoxin in Sepsis: Methods for LPS Detection and the Use of Omics Techniques. Diagnostics (Basel). 2022;13(1):79. doi:10.3390/diagnostics13010079
4. Yang L, Zhou R, Tong Y, et al. Neuroprotection by dihydrotestosterone in LPS-induced neuroinflammation. Neurobiol Dis. 2020;140:104814. doi:10.1016/j.nbd.2020.104814
5. Campbell JE, Newgard CB. Mechanisms controlling pancreatic islet cell function in insulin secretion. Nat Rev Mol Cell Biol. 2021;22(2):142-158. doi:10.1038/s41580-020-00317-7
6. Mehdi SF, Qureshi MH, Pervaiz S, et al. Endocrine and metabolic alterations in response to systemic inflammation and sepsis: a review article. Mol Med. 2025;31(1):16. doi:10.1186/s10020-025-01074-z
7. Kahles F, Meyer C, Möllmann J, et al. GLP-1 secretion is increased by inflammatory stimuli in an IL-6-dependent manner, leading to hyperinsulinemia and blood glucose lowering. Diabetes. 2014;63(10):3221-3229. doi:10.2337/db14-0100
8. Morikawa S, Kaneko N, Okumura C, et al. IAPP/amylin deposition, which is correlated with expressions of ASC and IL-1β in β-cells of Langerhans' islets, directly initiates NLRP3 inflammasome activation. Int J Immunopathol Pharmacol. 2018;32:2058738418788749. doi:10.1177/2058738418788749

9. Asci H, Savran M, Comlekci S, et al. Combined Pulsed Magnetic Field and Radiofrequency Electromagnetic Field Enhances MMP-9, Collagen-4, VEGF Synthesis to Improve Wound Healing Via Hif-1α/eNOS Pathway. Aesthetic Plast Surg. 2023;47(6):2841-2852. doi:10.1007/s00266-023-03450-8
10. Ma T, Ding Q, Liu C, Wu H. Electromagnetic fields regulate calcium-mediated cell fate of stem cells: osteogenesis, chondrogenesis and apoptosis. Stem Cell Res Ther. 2023;14(1):133. doi:10.1186/s13287-023-03303-w
11. Gunay I, Baldan I, Tokus M, Coskun C, Ocal I, Cicek FA. Pulsed magnetic field maintains vascular homeostasis against H2O2-induced oxidative stress. Gen Physiol Biophys. 2020;39(6):579-586. doi:10.4149/gpb_2020035
12. Zhou S, Wen H, He X, Han X, Li H. Pulsed electromagnetic field ameliorates the progression of osteoarthritis via the Sirt1/NF-κB pathway. Arthritis Res Ther. 2025;27(1):33. doi:10.1186/s13075-025-03492-0
13. Balci Ç, Özcan MS, Aşci H, et al. Radiofrequency Electromagnetic and Pulsed Magnetic Fields Protected the Kidney Against Lipopolysaccharide-Induced Acute Systemic Inflammation, Oxidative Stress, and Apoptosis by Regulating the IL-6/HIF1α/eNOS and Bcl2/Bax/Cas-9 Pathways. Medicina (Kaunas). 2025;61(2):238. doi:10.3390/medicina61020238
14. Wang G, Zhao X, He G, Li Z, Wang G, Lv Y. Effect of Butyric Acid on Apoptosis of Pancreatic β Cells. Ann Clin Lab Sci. 2025;55(2):259-267.
15. Ozmen O, Topsakal S. Preventive effect of agomelatine in lipopolysaccharide-induced pancreatic pathology. Drug Chem Toxicol. 2022;45(1):180-184. doi:10.1080/01480545.2019.1675686
16. Topsakal S, Ozmen O, Karakuyu NF, Bedir M, Sancer O. Cannabidiol Mitigates Lipopolysaccharide-Induced Pancreatic Pathology: A Promising Therapeutic Strategy. Cannabis Cannabinoid Res. 2024;9(3):809-818. doi:10.1089/can.2023.0153
17. Mukai E, Fujimoto S, Inagaki N. Role of Reactive Oxygen Species in Glucose Metabolism Disorder in Diabetic Pancreatic β-Cells. Biomolecules. 2022;12(9):1228. doi:10.3390/biom12091228
18. Miki A, Ricordi C, Sakuma Y, et al. Divergent antioxidant capacity of human islet cell subsets: A potential cause of beta-cell vulnerability in diabetes and islet transplantation. PLoS One. 2018;13(5):e0196570. doi:10.1371/journal.pone.0196570
19. Gualdi G, Costantini E, Reale M, Amerio P. Wound Repair and Extremely Low Frequency-Electromagnetic Field: Insight from In Vitro Study and Potential Clinical Application. Int J Mol Sci. 2021;22(9):5037. doi:10.3390/ijms22095037
20. Hernández Morales M, Shang T, Chen J, Han V, Liu C. Lipid Oxidation Induced by RF Waves and Mediated by Ferritin Iron Causes Activation of Ferritin-Tagged Ion Channels. Cell Rep. 2020;30(10):3250-3260.e7. doi:10.1016/j.celrep.2020.02.070
21. Jimenez H, Wang M, Zimmerman JW, et al. Tumour-specific amplitude-modulated radiofrequency electromagnetic fields induce differentiation of hepatocellular carcinoma via targeting Cav3.2 T-type voltage-gated calcium channels and Ca2+ influx. EBioMedicine. 2019;44:209-224. doi:10.1016/j.ebiom.2019.05.034
22. Jia Q, Tian F, Duan WN, Jia YF, Wang HX, Xia ZY. Effects of Dezocine-Remifentanil Intravenous Anaesthesia on Perioperative Signs, Serum TNF-&aipha; and IL-6 in Liver Cancer Patients undergoing Radiofrequency Ablation. J Coll Physicians Surg Pak. 2019;29(1):4-7. doi:10.29271/jcpsp.2019.01.4
23. Zheng Y, Huang Q, Zhang Y, et al. Multimodal roles of transient receptor potential channel activation in inducing pathological tissue scarification. Front Immunol. 2023;14:1237992. doi:10.3389/fimmu.2023.1237992
24. Zhao M, Liu A, Wu J, Mo L, Lu F, Wan G. Il1r2 and Tnfrsf12a in transcranial magnetic stimulation effect of ischemic stroke via bioinformatics analysis. Medicine (Baltimore). 2024;103(4):e36109. doi:10.1097/MD.0000000000036109
25. Falone S, Santini S Jr, Cordone V, et al. Extremely Low-Frequency Magnetic Fields and Redox-Responsive Pathways Linked to Cancer Drug Resistance: Insights from Co-Exposure-Based In Vitro Studies. Front Public Health. 2018;6:33. doi:10.3389/fpubh.2018.00033
26. Andreozzi F, Di Fatta C, Spiga R, et al. Glucagon induces the hepatic expression of inflammatory markers in vitro and in vivo. Diabetes Obes Metab. 2023;25(2):556-569. doi:10.1111/dom.14902
27. Hou X, Sun L, Li Z, et al. Associations of amylin with inflammatory markers and metabolic syndrome in apparently healthy Chinese. PLoS One. 2011;6(9):e24815. doi:10.1371/journal.pone.0024815
28. Mehdi SF, Qureshi MH, Pervaiz S, et al. Endocrine and metabolic alterations in response to systemic inflammation and sepsis: a review article. Mol Med. 2025;31(1):16. doi:10.1186/s10020-025-01074-z
29. Chen Q, Chen X, Jia Z, et al. ∆nFGF1 Protects β-Cells against High Glucose-Induced Apoptosis via the AMPK/SIRT1/PGC-1α Axis. Oxid Med Cell Longev. 2022;2022:1231970. doi:10.1155/2022/1231970
30. Hu G, Malik AB, Minshall RD. Toll-like receptor 4 mediates neutrophil sequestration and lung injury induced by endotoxin and hyperinflation. Crit Care Med. 2010;38(1):194-201. doi:10.1097/CCM.0b013e3181bc7c17