Evaluation of lymphocyte crossmatch (CDCXM and Flow XM) test results before kidney transplantation

Ergun Mete

doi:10.31362/patd.1825119

EN TR

Evaluation of lymphocyte crossmatch (CDCXM and Flow XM) test results before kidney transplantation

Abstract

Purpose: Detection of anti-HLA antibodies is crucial in pre-transplant immunological assessment to evaluate donor-recipient compatibility and prevent hyperacute rejection. This study aimed to evaluate lymphocyte crossmatch (XM) test results performed before kidney transplantation.
Materials and methods: Lymphocyte crossmatch tests conducted at the Tissue Typing Laboratory of Pamukkale University Hospital between February 2020 and February 2024 were retrospectively analyzed. CDCXM (Complement-Dependent Cytotoxicity Crossmatch) and flow cytometric crossmatch (FCXM) tests were performed. For CDCXM, T and B lymphocytes were separated and tested individually, with serial dilution and DTT treatment applied. FCXM used anti-CD3 for T lymphocytes, anti-CD19 and anti-IgG for B lymphocytes, analyzed on a 10-color, 3-laser flow cytometer (Navios EX-Beckman Coulter, Inc. USA).
Results: A total of 225 CDCXM test results, 105 of which were alive and 120 of which were cadaver, were included in the study. CDCXM T and FCXM T were negative in living donors, while CDCXM B and FCXM B positivity were 4.8% and 2.6%, respectively. CDCXM T and B positivity in cadaveric donors were 3.3% and 7.5%, respectively, and FCXM T and B positivity were 3.3% and 8.8%, respectively.
Conclusion: Lymphocyte crossmatch remains a reliable and classical method for pre-transplant immunological evaluation. It effectively detects donor-specific anti-HLA antibodies and contributes to the prevention of hyperacute rejection in kidney transplantation.

Keywords

Kidney transplantation, lymphocyte crossmatch, CDC crossmatch, flowcytometric crossmatch, anti-HLA antibodies

Böbrek nakli öncesi yapılan lenfosit crossmatch (CDCXM ve Flow XM) test sonuçlarının değerlendirilmesi

Abstract

Amaç: Anti-HLA antikorlarının tespiti, donör-alıcı uyumunu değerlendirmek ve hiperakut reddi önlemek için nakil öncesi immünolojik değerlendirmede kritik öneme sahiptir. Bu çalışmada, böbrek nakli öncesinde yapılan lenfosit çapraz uyum (XM) testi sonuçlarının değerlendirilmesi amaçlanmıştır.
Gereç ve yöntem: Şubat 2020 ile Şubat 2024 tarihleri arasında Pamukkale Üniversitesi Hastanesi Doku Tipleme Laboratuvarı'nda gerçekleştirilen lenfosit çapraz uyum testleri retrospektif olarak analiz edilmiştir. CDCXM (Komplement Bağımlı Sitotoksisite Çapraz Uyum) ve akım sitometrik çapraz uyum (FCXM) testleri gerçekleştirilmiştir. CDCXM için T ve B lenfositleri ayrı ayrı ayrı test edilmiş, seri dilüsyon ve DTT uygulanmıştır. FCXM'de T lenfositleri için anti-CD3, B lenfositleri için anti-CD19 ve anti-IgG kullanılmış ve 10 renkli, 3 lazerli akım sitometresinde (Navios EX-Beckman Coulter, Inc. USA) analiz edilmiştir.
Bulgular: Çalışmaya 105’i canlı ve 120’si kadavrada olmak üzere toplam Toplam 225 CDCXM test sonucu dahil edilmiştir. Canlı donörlerde CDCXM T ve FCXM T negatif bulunurken, CDCXM B ve FCXM B pozitifliği sırasıyla %4,8 ve %2,6 idi. Kadavra donörlerde CDCXM T ve B pozitifliği sırasıyla %3,3 ve %7,5, FCXM T ve B pozitifliği ise sırasıyla %3,3 ve %8,8 idi.
Sonuç: Lenfosit çapraz eşleştirme, nakil öncesi immünolojik değerlendirme için güvenilir ve klasik bir yöntem olmaya devam etmektedir. Vericiye özgü anti-HLA antikorlarını etkili bir şekilde tespit eder ve böbrek naklinde hiperakut reddin önlenmesine katkıda bulunur.

Keywords

Böbrek nakli, lenfosit çapraz eşleştirme, CDC çapraz eşleştirme, flowsitometrik çapraz eşleştirme, anti-HLA antikorları

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Ergun Mete ^*
0000-0002-0854-2440
Türkiye

Publication Date

April 13, 2026

Submission Date

November 17, 2025

Acceptance Date

March 18, 2026

Published in Issue

Year 2026 Volume: 19 Number: 2

DOI

https://doi.org/10.31362/patd.1825119

IZ

https://izlik.org/JA59NY35FG

APA

Mete, E. (2026). Evaluation of lymphocyte crossmatch (CDCXM and Flow XM) test results before kidney transplantation. Pamukkale Medical Journal, 19(2), 423-428. https://doi.org/10.31362/patd.1825119

AMA

1.Mete E. Evaluation of lymphocyte crossmatch (CDCXM and Flow XM) test results before kidney transplantation. Pam Med J. 2026;19(2):423-428. doi:10.31362/patd.1825119

Chicago

Mete, Ergun. 2026. “Evaluation of Lymphocyte Crossmatch (CDCXM and Flow XM) Test Results before Kidney Transplantation”. Pamukkale Medical Journal 19 (2): 423-28. https://doi.org/10.31362/patd.1825119.

EndNote

Mete E (April 1, 2026) Evaluation of lymphocyte crossmatch (CDCXM and Flow XM) test results before kidney transplantation. Pamukkale Medical Journal 19 2 423–428.

IEEE

[1]E. Mete, “Evaluation of lymphocyte crossmatch (CDCXM and Flow XM) test results before kidney transplantation”, Pam Med J, vol. 19, no. 2, pp. 423–428, Apr. 2026, doi: 10.31362/patd.1825119.

ISNAD

Mete, Ergun. “Evaluation of Lymphocyte Crossmatch (CDCXM and Flow XM) Test Results before Kidney Transplantation”. Pamukkale Medical Journal 19/2 (April 1, 2026): 423-428. https://doi.org/10.31362/patd.1825119.

JAMA

1.Mete E. Evaluation of lymphocyte crossmatch (CDCXM and Flow XM) test results before kidney transplantation. Pam Med J. 2026;19:423–428.

MLA

Mete, Ergun. “Evaluation of Lymphocyte Crossmatch (CDCXM and Flow XM) Test Results before Kidney Transplantation”. Pamukkale Medical Journal, vol. 19, no. 2, Apr. 2026, pp. 423-8, doi:10.31362/patd.1825119.

Vancouver

1.Ergun Mete. Evaluation of lymphocyte crossmatch (CDCXM and Flow XM) test results before kidney transplantation. Pam Med J. 2026 Apr. 1;19(2):423-8. doi:10.31362/patd.1825119

Pamukkale Medical Journal is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY NC).

Evaluation of lymphocyte crossmatch (CDCXM and Flow XM) test results before kidney transplantation

Abstract

Keywords

Böbrek nakli öncesi yapılan lenfosit crossmatch (CDCXM ve Flow XM) test sonuçlarının değerlendirilmesi

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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