The current study was carried out to determine the role of professional concern and university life quality of prospective teachers in explaining their attitudes towards the profession of teaching. The working group of the research was made up of 580 prospective teachers attending a faculty of education at a state university. As a data collection tool, “The Attitude Scale towards the Profession of Teaching”, “Occupational Anxiety Scale for Prospective Teachers” and “The Scale of Quality of Life in University” were used. The research was carried out through relational research method, a quantitative research method. At the end of the research, it was found that the professional attitudes of the prospective teachers were positive, their concern levels were low, they experienced “Appointment Centred Concern” and “Adaptation Centred Concern” the most, their satisfaction regarding university life quality was at medium level and that they were content with “Social Facilities” the least. It was found that there was a significant relation between the attitude towards profession and professional concern in a negative way and that as the professional concern decreased, positive professional attitude increased. It was also found that there was a significant relation between the attitude towards profession and university life quality in a positive way and that as the satisfaction of university life quality increased so did positive professional attitude. It was determined that there was a significant relation between the professional concern and university life quality in a negative way and that as the university life quality increased, professional concern decreased. It was determined that professional concern of the prospective teachers and their satisfaction regarding university life quality predicted the attitude towards the profession of teaching at 13.9% rate. In line with the results of the research, various recommendations were made.