Kalsiyum Fosfat ve Bitki Özüt Karışımlarının Diş Macununda Etkinlikleri

Year 2025, EARLY VIEW, 1 - 1

Erdem Sahin

https://doi.org/10.2339/politeknik.1535759

Abstract

Bu çalışmada diş macunu formülasyonlarında kullanılmak üzere doğal kaynaklardan kolaylıkla elde edilebilen kalsiyum fosfatların ve lokal bitki özütlerinin ağız florası üzerindeki etkisi incelenmiştir. Doğal hammaddelerle hazırlanan diş macunlarında aşındırıcı mineral olarak kullanılan iki kalsiyum fosfat fazının (hidroksiapatit ve monetit) optimum karışım kompozisyonu ve Ege bölgesinden çeşitli bitkilerin (zeytin, incir, ardıç, sakız, ıtır, yaban mersini yaprakları, nar kabuğu ve ardıç meyvesi) özütlerinin diş macunu antibakteriyel komponenti olarak etken konsantrasyonları mekanik (Vickers mikrosertlik) ve biyolojik (UV-spektrofotometre ile minimum inhibe edici konsantrasyon tespiti) testlerle incelenmiştir. Bu çerçevede ağız ortamını temsil eden Laktobasilus kasei bakteri ortamı içinde tutulan sentetik hidroksiapatit dişlere taşıyıcı macun vasıtası ile periyodik şekilde uygulanan kompozisyonların süreç sonunda diş sertliklerine etkisi belirlenmiştir. Ayrıca bitki özütlerinin yine ağız ortamında bulunan Streptokok mutans bakterisi üzerinde antibakteriyel etkileri seri seyreltme yöntemiyle belirlenmiştir. Sonuç olarak diş sertliği, dolayısıyla çürüme üzerinde en etkili diş macunları 3’e 1 monetit/hidroksiapatit karışımı ile yaban mersini veya ıtır yaprağı özütleri içeren formülasyonlar olarak bulunmuştur.

Keywords

hidroksiapatit , monetit , bitki özütü , antibacterial test , dişmacunu

Effectiveness of Calcium Phosphate and Plant Extract Mixtures as Natural Dentifrices

Abstract

This study investigates the effects of calcium phosphates and local plant extracts on the oral flora as naturally available ingredients for dentifrices. Effective compositions of two calcium phosphate minerals (hydroxyapatite and monetite) to serve as abrasive particles and extracts of plants (olive, fig, juniper, mastic, geranium, blueberry leaves, pomegranate peel and juniper fruit) obtained from Aegean region to serve as antibacterial agents in the as prepared natural dentifrices were investigated by mechanical (Vickers microhardness) and biological (minimum inhibitory concentrations from UV-spectrophotometry) tests. Within this framework, surface hardness of synthetic hydroxyapatite disks immersed in Lactobacillus casei cell culture was measured before and after periodic brushing with dentifrices containing calcium phosphates and plant extracts. Also in vitro serial dilution test was applied to plant extracts using Streptococcus mutans cell culture to determine their antibacterial activity. The results point to an optimum composition of 3 part monetite to 1 part hydroxyapatite powder mixtures and blueberry or geranium leaf extracts at their minimum inhibitory concentrations for highest hardness, i.e. lowest caries formation.

Keywords

Dentifrice , hydroxyapatite , monetite , plant extract , hardness test , antibacterial test

Ethical Statement

The author of this article declares that the materials and methods used in this study do not require ethical committee permission and/or legal-special permission.

Thanks

The author is grateful to Mrs. Gizem Dikici and Mr. Ahmet Erkin Ermiş for their assistance during the biological testing. Support of the Biochemistry laboratory staff at the Department of Chemical Engineering in İzmir Institute of Technology is also appreciated.

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